Mouse Monoclonal Antibody to TNF-alpha | ProMab 20161 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to TNF-alpha

catalog :

20161

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A9B9

The same clone is also sold as:

Invitrogen: MA5-15366
OriGene: AM06195PU-N

reactivity :

human

application :

western blot, ELISA

product information

Catalog :

20161

Title :

Mouse Monoclonal Antibody to TNF-alpha

Species :

Human

Clone :

2A9B9;

lsotype :

Mouse IgG1

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

TNF-alpha (tumor necrosis factor alpha) is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two, three, or five non covalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. Although it has little effect on many cultured normal human cells, TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF-alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Immunogen :

Recombinant Human TNF-alpha (BioSource company, Cat.No. PHC3013)

Formulation :

Purified antibody in PBS containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000

References :

1. Knowlton KU. Yajima T. J Am Coll Cardiol. 2004,Sep 15, 44(6):1298-300. ; 2. Reynolds JL. Ignatowski TA. Gallant S.et al. Brain Res. 2004,Oct 8, 1023(1):112-20. ; 3. Dulak J. Tomala K. Loboda A. et al. Life Sci. 2004,Oct 8, 75(21):2573-86. ;

Type :

Monoclonal Antibody

Aliases :

DIF; TNFA; TNFSF2; TNF-alpha