Mouse Monoclonal Antibody to PRAK | ProMab 20147 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to PRAK

catalog :

20147

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H10B4

The same clone is also sold as:

Santa Cruz Biotechnology: sc-81705
Abnova: MAB10404
Invitrogen: MA5-15352
OriGene: AM06183SU-N

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog :

20147

Title :

Mouse Monoclonal Antibody to PRAK

Species :

Human

Clone :

7H10B4;

lsotype :

Mouse IgG1

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38a and p38ß MAP kinases. Activated JNK, p38? or p38d are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.

Immunogen :

Purified recombinant fragment of PRAK expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000

References :

1. Paliga AJ. Natale DR. Watson AJ. Biol Cell. 2005, Aug, 97(8):629-40. ; 2. Wijtten PJ. Prak R. Lemme A. et al. Br Poult Sci. 2004, Aug, 45(4):504-11. ; 3. New L. Jiang Y. Han J. Mol Biol Cell. 2003, Jun, 14(6):2603-16. Epub 2003 Mar 20. ;

Type :

Monoclonal Antibody

Aliases :

PRAK; MAPKAPK5