Mouse Monoclonal Antibody to GFP | ProMab 20144 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to GFP

catalog :

20144

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4B10B2

application :

western blot, ELISA, immunohistochemistry, flow cytometry

product information

Catalog :

20144

Title :

Mouse Monoclonal Antibody to GFP

Clone :

4B10B2;

lsotype :

Mouse IgG2a

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Immunogen :

Purified recombinant fragment of GFP expressed in E. Coli.

Formulation :

Purified antibody in PBS containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. ; 2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. ; 3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68. ;

Type :

Monoclonal Antibody

MW :

27kDa