antibody

Mouse Monoclonal Antibody to IGF1R-Beta

20138

100µl

monoclonal

mouse

nonconjugated

3C8B1

The same clone is also sold as:

• OriGene: AM06175SU-N
• Invitrogen: MA5-15343
• Boster: M00070
• Abnova: MAB10692

western blot, ELISA, immunohistochemistry

Mouse Monoclonal Antibody to IGF1R-Beta

Human;

3C8B1;

Mouse IgG2b

100µl

4?; -20? for long term storage

IGF1R (insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Purified recombinant fragment of IGF1R-Beta expressed in E. Coli.

Ascitic fluid containing 0.03% sodium azide.

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000

1. Adams, T.E. et al. Cell. Mol. Life Sci. 2000 57, 1050-1093. ; 2. Baserga, R. et al. Oncogene 2000 19, 5574-5581. ; 3. Scheidegger, K.J. et al. J. Biol. Chem. 2000 275, 38921-38928. ;

Monoclonal Antibody

96kDa

IGF1R, IGF1R-Beta