Mouse Monoclonal Antibody to BTK | ProMab 20132 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to BTK

catalog :

20132

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7F12H4

The same clone is also sold as:

Santa Cruz Biotechnology: sc-81159
OriGene: TA336560
Abnova: MAB10456
Invitrogen: MA5-15337
Novus Biologicals: NBP2-80605, NB110-87047

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog :

20132

Title :

Mouse Monoclonal Antibody to BTK

Species :

Human; Monkey

Clone :

7F12H4;

lsotype :

Mouse IgG1

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Immunogen :

Purified recombinant fragment of BTK expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000

References :

1. Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240. ; 2. Thomas, J.D., et al. 1993. Science. 261: 355-358. ; 3. Tamagnone, L., et al. Oncogene 9: 3683-3688. ;

Type :

Monoclonal Antibody

MW :

77kDa

Aliases :

BTK