Mouse Monoclonal Antibody to BRAF | ProMab 20108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to BRAF

catalog :

20108

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H12F1

The same clone is also sold as:

OriGene: AM06152SU-N
Abnova: MAB10455
Novus Biologicals: NBP1-47555

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog :

20108

Title :

Mouse Monoclonal Antibody to BRAF

Species :

Human

Clone :

1H12F1;

lsotype :

Mouse IgG1

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

BRAF(V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.

Immunogen :

Purified recombinant fragment of BRAF expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000

References :

1. Rapp, U.R.,et al.1983.Proc. Natl.Acad.Sci.USA.80:4218-4222. ; 2. Kim J,Giuliano AE,Turner RR.2006.Ann Surg.Nov, 244(5): 799-804. ; 3. Fullen DR, Poynter JN, Lowe L,2006.Mod Pathol. 19(10): 1324-1332. ; 4. Terai K, Matsuda M.2006.MBO J.25(15):3556-3564. ; 5. Noda H,Kato Y,Yoshikawa H,2006.J Exp Clin Cancer Res. 25(2):235-242. ;

Type :

Monoclonal Antibody

Aliases :

BRAF