Mouse Monoclonal Antibody to ELK1 | ProMab 20101 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to ELK1

catalog :

20101

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H6D12

The same clone is also sold as:

OriGene: AM06146SU-N
Santa Cruz Biotechnology: sc-65986

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog :

20101

Title :

Mouse Monoclonal Antibody to ELK1

Species :

Human

Clone :

3H6D12;

lsotype :

Mouse IgG1

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

Immunogen :

Purified recombinant fragment of ELK1 expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000

References :

1. Rao,V.N., et al. 1989.Science.244 (4900):66-70. ; 2. Hsieh,Y.H., et al. 2006.Biochem. Biophys. Res. Commun. 339 (1): 217-225. ; 3. Gille,H., Strahl,T. and Shaw,P.E.1995. Curr. Biol. 5 (10): 1191-1200. ; 4. Gille,H., et al. 1995.EMBO J. 14 (5): 951-962. ;

Type :

Monoclonal Antibody

Aliases :

ELK1