Western blot with Porcine plasma and purified Porcine CRP (poCRP). Porcine plasma was diluted 1/25 and spiked with 0.08 or 0.3ug purified Porcine CRP, respectively. Polyacrylamide gel electrophoresis was performed under reducing and denaturing conditions on a 10-20% gel (Novex), blotted onto nitrocellulose and developed with monoclonal antibody PC1 (left) or competitor's monoclonal antibody (right). Lane A: Porcine plasma spiked with 0.08ug poCRP; lane B: 0.08ug poCRP; lane C: Porcine plasma spiked with 0.3ug poCRP; lane D: Porcine plasma; lane E: 0.3ug poCRP. Monoclonal antibody PC1 barely reacts with with the 50-55kDa bands visible on the right, presumably immunoglobulin heavy chains. Gel was slightly overloaded to allow visibility of CRP band in plasma.