domestic goat polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
mouse monoclonal (2A5)
reactivity: human
application: western blot, ELISA, immunocytochemistry
reactivity: human
application: western blot, ELISA, immunocytochemistry
Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2A5. For analysis HRP-conjugated 2A5 antibody was used. 200 ng of purified recombinant Artemin expressed by CHOEBNALT85 cell line was loaded per lane. Lane 1: Reduced conditions; Line 2: Non-reduced conditions; Lane 3: Protein marker (8003, Naxo)
quantity: 100 µg
price: 522 USD
to the supplier
mouse monoclonal (2D12)
reactivity: human
application: western blot, ELISA, immunocytochemistry
reactivity: human
application: western blot, ELISA, immunocytochemistry
Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2D12. Goat anti-mouse IgG HRP conjugate was used as secondary antibody. 15 µl of CHOEBNALT85 Artemin expressing supernatant was loaded per line. Line 1. Reduced conditions; Line 2. Non-reduced conditions; Line 3. Protein marker (8003, Naxo).
quantity: 100 µg
price: 522 USD
to the supplier
mouse monoclonal (1F3)
reactivity: human
application: ELISA, immunocytochemistry
reactivity: human
application: ELISA, immunocytochemistry
Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 1F3. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells.
quantity: 100 µg
price: 522 USD
to the supplier
mouse monoclonal (2G7)
reactivity: human
application: ELISA, immunocytochemistry
reactivity: human
application: ELISA, immunocytochemistry
Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2G7. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells
quantity: 100 µg
price: 522 USD
to the supplier