product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free
catalog :
NBP2-36440
quantity :
0.1 ml (also 0.025 ml)
price :
449 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7A9-3A3
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig s6b
Oakes B, Fellmann C, Rishi H, Taylor K, Ren S, Nadler D, et al. CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Cell. 2019;176:254-267.e16 pubmed publisher
  • western blot; human; loading ...; fig 2c
Charpentier M, Khedher A, Menoret S, Brion A, Lamribet K, Dardillac E, et al. CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nat Commun. 2018;9:1133 pubmed publisher
Ilahibaks N, Kluiver T, de Jong O, de Jager S, Schiffelers R, Vader P, et al. Extracellular vesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex targeting proprotein convertase subtilisin-kexin type 9 (Pcsk9) in primary mouse hepatocytes. J Extracell Vesicles. 2024;13:e12389 pubmed publisher
Pettitt S, Shao N, Zatreanu D, Frankum J, Bajrami I, Brough R, et al. A HUWE1 defect causes PARP inhibitor resistance by modulating the BRCA1-∆11q splice variant. Oncogene. 2023;42:2701-2709 pubmed publisher
Zelceski A, Francica P, Lingg L, Mutlu M, Stok C, Liptay M, et al. MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells. Cell Rep. 2023;42:112484 pubmed publisher
Tanaka M, Chuaychob S, Homme M, Yamazaki Y, Lyu R, Yamashita K, et al. ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma. Nat Commun. 2023;14:1957 pubmed publisher
Szczerba M, Johnson B, Acciai F, Gogerty C, McCaughan M, Williams J, et al. Canonical cellular stress granules are required for arsenite-induced necroptosis mediated by Z-DNA-binding protein 1. Sci Signal. 2023;16:eabq0837 pubmed publisher
Zhang C, Schekman R. Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein. elife. 2023;12: pubmed publisher
Zhang Y, Rabinovsky R, Wei Z, El Fatimy R, Deforzh E, Luan B, et al. Secreted PGK1 and IGFBP2 contribute to the bystander effect of miR-10b gene editing in glioma. Mol Ther Nucleic Acids. 2023;31:265-275 pubmed publisher
Cappellesso F, Orban M, Shirgaonkar N, Berardi E, Serneels J, Neveu M, et al. Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer. Nat Cancer. 2022;3:1464-1483 pubmed publisher
Abrego J, Sanford Crane H, Oon C, Xiao X, Betts C, Sun D, et al. A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor Immunity. Cancer Discov. 2022;12:2414-2433 pubmed publisher
Sakashita A, Ariura M, Namekawa S. CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells. Methods Mol Biol. 2022;2509:171-194 pubmed publisher
Unlu G, Prizer B, Erdal R, Yeh H, Bayraktar E, Birsoy K. Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. Mol Cell. 2022;: pubmed publisher
Park S, Uchida T, Tilson S, Hu Z, Ma C, Leek M, et al. A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells. Mol Ther Nucleic Acids. 2022;28:656-669 pubmed publisher
Economos N, Quijano E, Carufe K, Perera J, Glazer P. Antispacer peptide nucleic acids for sequence-specific CRISPR-Cas9 modulation. Nucleic Acids Res. 2022;50:e59 pubmed publisher
Bozoglu T, Lee S, Ziegler T, Jurisch V, Maas S, Baehr A, et al. Endothelial Retargeting of AAV9 In Vivo. Adv Sci (Weinh). 2022;9:e2103867 pubmed publisher
Uchida T, Park S, Inuzuka T, Zhang M, Allen J, Chayama K, et al. Genetically edited hepatic cells expressing the NTCP-S267F variant are resistant to hepatitis B virus infection. Mol Ther Methods Clin Dev. 2021;23:597-605 pubmed publisher
Bailey M, Tieu D, Habsid A, Tong A, Chan K, Moffat J, et al. Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2. Life Sci Alliance. 2021;4: pubmed publisher
Ah Fong A, Boyd A, Matson M, Judelson H. A Cas12a-based gene editing system for Phytophthora infestans reveals monoallelic expression of an elicitor. Mol Plant Pathol. 2021;22:737-752 pubmed publisher
Lamb A, Wang Z, Simmer P, Chung H, Wittkopp P. ebony affects pigmentation divergence and cuticular hydrocarbons in Drosophila americana and D. novamexicana. Front Ecol Evol. 2020;8: pubmed publisher
Zhao X, Jin C, Dong T, Sun Z, Zheng X, Feng B, et al. Characterization of promoters for adeno-associated virus mediated efficient Cas9 activation in adult Cas9 knock-in murine cochleae. Hear Res. 2020;394:107999 pubmed publisher
Alix E, Godlee C, Cerny O, Blundell S, Tocci R, Matthews S, et al. The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules. Cell Host Microbe. 2020;28:54-68.e7 pubmed publisher
Liu Y, Chen J, Crisante D, Jaramillo Lopez J, Mahadevan R. Dynamic Cell Programming with Quorum Sensing-Controlled CRISPRi Circuit. ACS Synth Biol. 2020;9:1284-1291 pubmed publisher
Yu Y, Leete T, Born D, Young L, Barrera L, Lee S, et al. Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity. Nat Commun. 2020;11:2052 pubmed publisher
Kang W, Zhao X, Sun Z, Dong T, Jin C, Tong L, et al. Adeno-associated virus vector enables safe and efficient Cas9 activation in neonatal and adult Cas9 knockin murine cochleae. Gene Ther. 2020;: pubmed publisher
Wu Y, Yang L, Chang T, Kandeel F, Yee J. A Small Molecule-Controlled Cas9 Repressible System. Mol Ther Nucleic Acids. 2020;19:922-932 pubmed publisher
Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, et al. Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity. Sci Rep. 2020;10:651 pubmed publisher
Li X. Gap junction protein connexin43 and tunneling nanotubes in human trabecular meshwork cells. Int J Physiol Pathophysiol Pharmacol. 2019;11:212-219 pubmed
Schick S, Rendeiro A, Runggatscher K, Ringler A, Boidol B, Hinkel M, et al. Systematic characterization of BAF mutations provides insights into intracomplex synthetic lethalities in human cancers. Nat Genet. 2019;51:1399-1410 pubmed publisher
Johnston R, Seamon K, Saada E, Podlevsky J, Branda S, Timlin J, et al. Use of anti-CRISPR protein AcrIIA4 as a capture ligand for CRISPR/Cas9 detection. Biosens Bioelectron. 2019;141:111361 pubmed publisher
Savage A, Kurusamy S, Chen Y, Jiang Z, Chhabria K, MacDonald R, et al. tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis. Nat Commun. 2019;10:732 pubmed publisher
Qiang J, Ma Z, Xie X, Shi L, Geng Y, Hu J, et al. Multi-omic Analyses Reveal Minimal Impact of the CRISPR-Cas9 Nuclease on Cultured Human Cells. J Proteome Res. 2019;18:1054-1063 pubmed publisher
Melnik S, Dvornikov D, Muller Decker K, Depner S, Stannek P, Meister M, et al. Cancer cell specific inhibition of Wnt/?-catenin signaling by forced intracellular acidification. Cell Discov. 2018;4:37 pubmed publisher
Sykora P, Witt K, Revanna P, Smith Roe S, Dismukes J, Lloyd D, et al. Next generation high throughput DNA damage detection platform for genotoxic compound screening. Sci Rep. 2018;8:2771 pubmed publisher
Basila M, Kelley M, Smith A. Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity. PLoS ONE. 2017;12:e0188593 pubmed publisher
Rogers Z, McFarland C, Winters I, Naranjo S, Chuang C, Petrov D, et al. A quantitative and multiplexed approach to uncover the fitness landscape of tumor suppression in vivo. Nat Methods. 2017;14:737-742 pubmed publisher
El Fatimy R, Subramanian S, Uhlmann E, Krichevsky A. Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b. Mol Ther. 2017;25:368-378 pubmed publisher
Chew W, Tabebordbar M, Cheng J, Mali P, Wu E, Ng A, et al. A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016;13:868-74 pubmed publisher
Choi J, Dang Y, Abraham S, Ma H, Zhang J, Guo H, et al. Lentivirus pre-packed with Cas9 protein for safer gene editing. Gene Ther. 2016;23:627-33 pubmed publisher
Chu V, Weber T, Graf R, Sommermann T, Petsch K, Sack U, et al. Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016;16:4 pubmed publisher
Chiou S, Winters I, Wang J, Naranjo S, Dudgeon C, Tamburini F, et al. Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015;29:1576-85 pubmed publisher
product information
brand :
Novus Biologicals, a Bio-Techne Brand
catalog number base :
NBP2-36440
SKU :
NBP2-36440
product name :
CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free
Description :
The CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free from Novus is a mouse monoclonal antibody to CRISPR-Cas9. This antibody reacts with bacteria. The CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free has been validated for the following applications: Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Immunoblotting, Immunohistochemistry Whole-Mount, Knockout Validated.
target :
CRISPR-Cas9
category :
Primary Antibodies
unit size :
0.1 ml (also 0.025 ml)
buffer :
PBS
clonality :
Monoclonal
clone :
7A9-3A3
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
This CRISPR-Cas9 antibody (7A9-3A3) - N-Terminus was raised against Recombinant Cas9 within the N-terminal region of Streptococcus pyogene. [Uniprot: Q99ZW2].
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Bacteria
specificity :
This CRISPR-Cas9 antibody (7A9-3A3) - N-Terminus is specific toCas9 protein from Streptococcus pyogene serotype M1
theoretical molecular weight :
158.4 kDa
applications :
Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Immunoblotting, Immunohistochemistry Whole-Mount, Knockout Validated
USD :
449 USD
alt names :
Cas9, CRISPR, CRISPR-associated protein 9 nuclease
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.