IKK alpha Antibody (14A231) - Azide Free | Novus Biologicals NBP2-27409 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

IKK alpha Antibody (14A231) - Azide Free

catalog :

NBP2-27409

quantity :

0.1 mg

price :

469 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14A231

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Novus Biologicals product webpage

citations: 41

Reference
Todoric J, Antonucci L, Di Caro G, Li N, Wu X, Lytle N, et al. Stress-Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas. Cancer Cell. 2017;32:824-839.e8 pubmed publisher
Cui J, Li Y, Zhu L, Liu D, Songyang Z, Wang H, et al. NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4. Nat Immunol. 2012;13:387-95 pubmed publisher
Bakkar N, Ladner K, Canan B, Liyanarachchi S, Bal N, Pant M, et al. IKK? and alternative NF-?B regulate PGC-1? to promote oxidative muscle metabolism. J Cell Biol. 2012;196:497-511 pubmed publisher
Delhase M, Kim S, Lee H, Naiki Ito A, Chen Y, Ahn E, et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012;109:E177-86 pubmed publisher
Harikumar K, Sung B, Pandey M, Guha S, Krishnan S, Aggarwal B. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010;77:818-27 pubmed publisher
Huang J, Ren T, Guan H, Jiang Y, Cheng H. HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB. J Biol Chem. 2009;284:6208-17 pubmed publisher
Eckmann L, Nebelsiek T, Fingerle A, Dann S, Mages J, Lang R, et al. Opposing functions of IKKbeta during acute and chronic intestinal inflammation. Proc Natl Acad Sci U S A. 2008;105:15058-63 pubmed publisher
Otipoby K, Sasaki Y, Schmidt Supprian M, Patke A, Gareus R, Pasparakis M, et al. BAFF activates Akt and Erk through BAFF-R in an IKK1-dependent manner in primary mouse B cells. Proc Natl Acad Sci U S A. 2008;105:12435-8 pubmed publisher
Pandey M, Sung B, Kunnumakkara A, Sethi G, Chaturvedi M, Aggarwal B. Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis. Cancer Res. 2008;68:5370-9 pubmed publisher
Kasinski A, Du Y, Thomas S, Zhao J, Sun S, Khuri F, et al. Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin. Mol Pharmacol. 2008;74:654-61 pubmed publisher
Beraza N, Ludde T, Assmus U, Roskams T, Vander Borght S, Trautwein C. Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury. Gastroenterology. 2007;132:2504-17 pubmed
Jackson Bernitsas D, Ichikawa H, Takada Y, Myers J, Lin X, Darnay B, et al. Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma. Oncogene. 2007;26:1385-97 pubmed
Sethi G, Ahn K, Sandur S, Lin X, Chaturvedi M, Aggarwal B. Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway. J Biol Chem. 2006;281:23425-35 pubmed
Ichikawa H, Aggarwal B. Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation. Clin Cancer Res. 2006;12:662-8 pubmed
Vinolo E, Sebban H, Chaffotte A, Israel A, Courtois G, Veron M, et al. A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis factor-mediated NF-kappa B activation. J Biol Chem. 2006;281:6334-48 pubmed
Ichikawa H, Takada Y, Murakami A, Aggarwal B. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005;174:7383-92 pubmed
Ruocco M, Maeda S, Park J, Lawrence T, Hsu L, Cao Y, et al. I{kappa}B kinase (IKK){beta}, but not IKK{alpha}, is a critical mediator of osteoclast survival and is required for inflammation-induced bone loss. J Exp Med. 2005;201:1677-87 pubmed
Nonaka M, Horie R, Itoh K, Watanabe T, Yamamoto N, Yamaoka S. Aberrant NF-kappaB2/p52 expression in Hodgkin/Reed-Sternberg cells and CD30-transformed rat fibroblasts. Oncogene. 2005;24:3976-86 pubmed
Panopoulos A, Harraz M, Engelhardt J, Zandi E. Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB activation. J Biol Chem. 2005;280:2912-23 pubmed
Shishodia S, Aggarwal B. Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem. 2004;279:47148-58 pubmed
Shishodia S, Koul D, Aggarwal B. Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis. J Immunol. 2004;173:2011-22 pubmed
Takada Y, Fang X, Jamaluddin M, Boyd D, Aggarwal B. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004;279:39541-54 pubmed
Chandrasekar B, Vemula K, Surabhi R, Li Weber M, Owen Schaub L, Jensen L, et al. Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death. J Biol Chem. 2004;279:20221-33 pubmed
Takada Y, Singh S, Aggarwal B. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004;279:15096-104 pubmed
Takada Y, Aggarwal B. Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9. J Biol Chem. 2004;279:4750-9 pubmed
Saito N, Courtois G, Chiba A, Yamamoto N, Nitta T, Hironaka N, et al. Two carboxyl-terminal activation regions of Epstein-Barr virus latent membrane protein 1 activate NF-kappaB through distinct signaling pathways in fibroblast cell lines. J Biol Chem. 2003;278:46565-75 pubmed
Chen L, Egan L, Li Z, Greten F, Kagnoff M, Karin M. The two faces of IKK and NF-kappaB inhibition: prevention of systemic inflammation but increased local injury following intestinal ischemia-reperfusion. Nat Med. 2003;9:575-81 pubmed
Majumdar S, Aggarwal B. Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types. Oncogene. 2003;22:1206-18 pubmed
Ashikawa K, Majumdar S, Banerjee S, Bharti A, Shishodia S, Aggarwal B. Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol. 2002;169:6490-7 pubmed
Chinanonwait N, Miura H, Yamamoto N, Yamaoka S. A recessive mutant cell line with a constitutive IkappaB kinase activity. FEBS Lett. 2002;531:553-60 pubmed
Dejardin E, Droin N, Delhase M, Haas E, Cao Y, Makris C, et al. The lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways. Immunity. 2002;17:525-35 pubmed
Makris C, Roberts J, Karin M. The carboxyl-terminal region of IkappaB kinase gamma (IKKgamma) is required for full IKK activation. Mol Cell Biol. 2002;22:6573-81 pubmed
Anto R, Mukhopadhyay A, Shishodia S, Gairola C, Aggarwal B. Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2. Carcinogenesis. 2002;23:1511-8 pubmed
Majumdar S, Lamothe B, Aggarwal B. Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester. J Immunol. 2002;168:2644-51 pubmed
Agou F, Ye F, Goffinont S, Courtois G, Yamaoka S, Israel A, et al. NEMO trimerizes through its coiled-coil C-terminal domain. J Biol Chem. 2002;277:17464-75 pubmed
Cao Y, Bonizzi G, Seagroves T, Greten F, Johnson R, Schmidt E, et al. IKKalpha provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development. Cell. 2001;107:763-75 pubmed
Majumdar S, Aggarwal B. Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation. J Immunol. 2001;167:2911-20 pubmed
Miller B, Zandi E. Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation. J Biol Chem. 2001;276:36320-6 pubmed
Koul D, Yao Y, Abbruzzese J, Yung W, Reddy S. Tumor suppressor MMAC/PTEN inhibits cytokine-induced NFkappaB activation without interfering with the IkappaB degradation pathway. J Biol Chem. 2001;276:11402-8 pubmed
Manna S, Mukhopadhyay A, Aggarwal B. Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis. J Immunol. 2000;165:5962-9 pubmed
Manna S, Mukhopadhyay A, Aggarwal B. IFN-alpha suppresses activation of nuclear transcription factors NF-kappa B and activator protein 1 and potentiates TNF-induced apoptosis. J Immunol. 2000;165:4927-34 pubmed

product information

master code :

NBP2-27409

SKU :

NBP2-27409

product name :

IKK alpha Antibody (14A231) - Azide Free

unit size :

0.1 mg

description :

The IKK alpha Antibody (14A231) - Azide and BSA Free from Novus is a mouse monoclonal antibody to IKK alpha. This antibody reacts with human,mouse,primate,rat. The IKK alpha Antibody (14A231) - Azide and BSA Free has been validated for the following applications: Western Blot,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Flow (Intracellular),Simple Western,CyTOF-ready,Immunocytochemistry/ Immunofluorescence.

target :

IKK alpha

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

14A231

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

This antibody was raised against a His-tagged full-length human IKK alpha protein.

isotype :

IgG1 Kappa

purity :

Protein G purified

species :

Human,Mouse,Primate,Rat

gene symbol :

CHUK

applications :

Western Blot,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Flow (Intracellular),Simple Western,CyTOF-ready,Immunocytochemistry/ Immunofluorescence

USD :

469 USD

alt names :

conserved helix-loop-helix ubiquitous kinaseIKBKA, EC 2.7.11, EC 2.7.11.10, IkappaB kinase, I-kappa-B kinase 1, I-kappa-B kinase alpha, IkBKA, IKK1I-kappa-B kinase-alpha, IKK-A, IKKAIkB kinase alpha subunit, IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKAIKK-a kinase, Nuclear factor NFkappaB inhibitor kinase alpha, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16, TCF-16, Transcription factor 16

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage