product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
GAPDH Antibody (13H12) - BSA Free
catalog :
NBP2-27103
quantity :
0.1 mg (also 0.025 mg)
price :
419 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13H12
reactivity :
primate, human, mouse, rat, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; mouse
Helmering J, Juan T, Li C, Chhoa M, Baron W, Gyuris T, et al. A mutation in Ampd2 is associated with nephrotic syndrome and hypercholesterolemia in mice. Lipids Health Dis. 2014;13:167 pubmed publisher
  • western blot; rat
  • western blot; human
Liu J, Pasini S, Shelanski M, Greene L. Activating transcription factor 4 (ATF4) modulates post-synaptic development and dendritic spine morphology. Front Cell Neurosci. 2014;8:177 pubmed publisher
  • western blot; primate
Das I, Basantray I, Mamidi P, Nayak T, B M P, Chattopadhyay S, et al. Heat shock protein 90 positively regulates Chikungunya virus replication by stabilizing viral non-structural protein nsP2 during infection. PLoS ONE. 2014;9:e100531 pubmed publisher
  • western blot; human
Mellergaard M, Skovbakke S, Schneider C, Lauridsen F, Andresen L, Jensen H, et al. N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24. J Biol Chem. 2014;289:20078-91 pubmed publisher
  • western blot; human
  • western blot; mouse
Pradhan N, Pratheek B, Garai A, Kumar A, Meena V, Ghosh S, et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol Int. 2014;38:1118-31 pubmed publisher
  • western blot; mouse
  • western blot; primate
Chattopadhyay S, Kumar A, Mamidi P, Nayak T, Das I, Chhatai J, et al. Development and characterization of monoclonal antibody against non-structural protein-2 of Chikungunya virus and its application. J Virol Methods. 2014;199:86-94 pubmed publisher
Kate W, Fanta M, Weinfeld M. Loss of the DNA repair protein, polynucleotide kinase/phosphatase, activates the type 1 interferon response independent of ionizing radiation. Nucleic Acids Res. 2024;52:9630-9653 pubmed publisher
Balint V, Perić M, Dacic S, Stanisavljevic Ninkovic D, Marjanovic J, Popovic J, et al. The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. Biomedicines. 2024;12: pubmed publisher
Guo D, Liu S, Zhang J, Gu X, Shi L, Su Y, et al. Prickle1-driven basement membrane deposition of the iPSC-derived embryoid bodies is separable from the establishment of apicobasal polarity. Cell Prolif. 2024;57:e13595 pubmed publisher
Wang C, Terrigno M, Li J, Distler T, Pandya N, Ebeling M, et al. Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation. Neuron. 2023;111:2660-2674.e9 pubmed publisher
Lin X, Fu B, Xiong Y, Xing N, Xue W, Guo D, et al. Unconventional secretion of unglycosylated ORF8 is critical for the cytokine storm during SARS-CoV-2 infection. PLoS Pathog. 2023;19:e1011128 pubmed publisher
Grotheer V, Skrynecki N, Oezel L, Windolf J, Grassmann J. Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence. Sci Rep. 2021;11:11968 pubmed publisher
Venkatramanan S, Ibar C, Irvine K. TRIP6 is required for tension at adherens junctions. J Cell Sci. 2021;: pubmed publisher
Krassovka J, Suschek C, Prost M, Grotheer V, Schiefer J, Demir E, et al. The impact of non-toxic blue light (453 nm) on cellular antioxidative capacity, TGF-β1 signaling, and myofibrogenesis of human skin fibroblasts. J Photochem Photobiol B. 2020;209:111952 pubmed publisher
Modi A, Singh M, Gutti G, Shanker O, Singh V, Singh S, et al. Benzothiazole derivative bearing amide moiety induces p53-mediated apoptosis in HPV16 positive cervical cancer cells. Invest New Drugs. 2019;: pubmed publisher
Srivastava S, Battu M, Khan M, Nandicoori V, Mukhopadhyay S. Mycobacterium tuberculosis PPE2 Protein Interacts with p67phox and Inhibits Reactive Oxygen Species Production. J Immunol. 2019;203:1218-1229 pubmed publisher
Eiraku N, Chiba N, Nakamura T, Amir M, Seong C, Ohnishi T, et al. BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts. FASEB J. 2019;:fj201900733RR pubmed publisher
Mishra P, Paital B, Jena S, Swain S, Kumar S, Yadav M, et al. Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NFĸB/AKT/mTOR/KEAP1 signalling in rat heart. Sci Rep. 2019;9:7408 pubmed publisher
Shwetha S, Sharma G, Raheja H, Goel A, Aggarwal R, Das S. Interaction of miR-125b-5p with Human antigen R mRNA: Mechanism of controlling HCV replication. Virus Res. 2018;258:1-8 pubmed publisher
van Deel E, Octavia Y, de Waard M, de Boer M, Duncker D. Exercise Training Has Contrasting Effects in Myocardial Infarction and Pressure Overload Due to Divergent Endothelial Nitric Oxide Synthase Regulation. Int J Mol Sci. 2018;19: pubmed publisher
Ibar C, Kirichenko E, Keepers B, Enners E, Fleisch K, Irvine K. Tension-dependent regulation of mammalian Hippo signaling through LIMD1. J Cell Sci. 2018;131: pubmed publisher
Winzi M, Casas Vila N, Paszkowski Rogacz M, Ding L, Noack S, Theis M, et al. The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells. PLoS ONE. 2018;13:e0191682 pubmed publisher
Padhy B, Hayat B, Nanda G, Mohanty P, Alone D. Pseudoexfoliation and Alzheimer's associated CLU risk variant, rs2279590, lies within an enhancer element and regulates CLU, EPHX2 and PTK2B gene expression. Hum Mol Genet. 2017;26:4519-4529 pubmed publisher
Sarabhai T, Peter C, Bär A, Windolf J, Relja B, Wesselborg S, et al. Serum α-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome. PLoS ONE. 2017;12:e0177450 pubmed publisher
Yeyati P, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams I, et al. KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability. J Cell Biol. 2017;216:999-1013 pubmed publisher
Bologna Molina R, Takeda Y, Kuga T, Chosa N, Kitagawa M, Takata T, et al. Expression of Wilms' tumor 1 (WT1) in ameloblastomas. J Oral Sci. 2016;58:407-13 pubmed publisher
Titos E, Rius B, López Vicario C, Alcaraz Quiles J, García Alonso V, Lopategi A, et al. Signaling and Immunoresolving Actions of Resolvin D1 in Inflamed Human Visceral Adipose Tissue. J Immunol. 2016;197:3360-3370 pubmed
Mishra D, Singh S, Narayan G. Curcumin Induces Apoptosis in Pre-B Acute Lymphoblastic Leukemia Cell Lines Via PARP-1 Cleavage. Asian Pac J Cancer Prev. 2016;17:3865-9 pubmed
Spasova M, Chen X, Sadowska G, Horton E, Lim Y, Stonestreet B. Ischemia reduces inter-alpha inhibitor proteins in the brain of the ovine fetus. Dev Neurobiol. 2017;77:726-737 pubmed publisher
Grimberg Peters D, Büren C, Windolf J, Wahlers T, Paunel Görgülü A. Hyperbaric Oxygen Reduces Production of Reactive Oxygen Species in Neutrophils from Polytraumatized Patients Yielding in the Inhibition of p38 MAP Kinase and Downstream Pathways. PLoS ONE. 2016;11:e0161343 pubmed publisher
Srinivas C, Ramaiah M, Lavanya A, Yerramsetty S, Kavi Kishor P, Basha S, et al. Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer. PLoS ONE. 2015;10:e0142006 pubmed publisher
Sun S, Reddy B, Irvine K. Localization of Hippo signalling complexes and Warts activation in vivo. Nat Commun. 2015;6:8402 pubmed publisher
Bernardes D, Brambilla R, Bracchi Ricard V, Karmally S, Dellarole A, Carvalho Tavares J, et al. Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis. J Neurochem. 2016;136 Suppl 1:63-73 pubmed publisher
Kumar S, Mamidi P, Kumar A, Basantray I, Bramha U, Dixit A, et al. Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research. Arch Virol. 2015;160:2749-61 pubmed publisher
Padiya R, Chowdhury D, Borkar R, Srinivas R, Pal Bhadra M, Banerjee S. Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat. PLoS ONE. 2014;9:e94228 pubmed publisher
product information
master code :
NBP2-27103
SKU :
NBP2-27103
product name :
GAPDH Antibody (13H12) - BSA Free
unit size :
0.1 mg (also 0.025 mg)
description :
The GAPDH Antibody (13H12) - BSA Free from Novus is a mouse monoclonal antibody to GAPDH. This antibody reacts with drosophila,human,insect - drosophila,monkey,mouse,ovine,primate,primate - macaca mulatta (rhesus macaque),rat,sheep. The GAPDH Antibody (13H12) - BSA Free has been validated for the following applications: IF/IHC,Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry/ Immunofluorescence.
target :
GAPDH
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
13H12
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
Amino acids between 275 and 325 of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this GAPDH antibody.
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Drosophila,Human,Insect - Drosophila,Monkey,Mouse,Ovine,Primate,Primate - Macaca mulatta (Rhesus Macaque),Rat,Sheep
theoretical molecular weight :
36 kDa
gene symbol :
GAPDH
accessionNumbers :
P04406
applications :
IF/IHC,Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry/ Immunofluorescence
USD :
419 USD
alt names :
aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.