Ubiquilin 2 Antibody (6H9) - BSA Free | Novus Biologicals NBP2-25164 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Ubiquilin 2 Antibody (6H9) - BSA Free

catalog :

NBP2-25164

quantity :

0.1 ml (also 0.025 ml)

price :

399 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6H9

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Novus Biologicals product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:2000; loading ...; fig 6h immunocytochemistry; human; 1:1000; loading ...; fig 1e	Mohan H, Trzeciakiewicz H, Pithadia A, Crowley E, Pacitto R, Safren N, et al. RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control. Cell Mol Life Sci. 2022;79:176 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1, 3 western blot; mouse; loading ...; fig s11a, s12a	Higgins N, Greenslade J, Wu J, Miranda E, Galliciotti G, Monteiro M. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD. Brain Pathol. 2021;:e12948 pubmed publisher
	Nementzik L, Thumbadoo K, Murray H, Gordon D, Yang S, Blair I, et al. Distribution of ubiquilin 2 and TDP-43 aggregates throughout the CNS in UBQLN2 p.T487I-linked amyotrophic lateral sclerosis and frontotemporal dementia. Brain Pathol. 2024;34:e13230 pubmed publisher
	Dao T, Rajendran A, Galagedera S, Haws W, Casta xf1 eda C. Short disordered termini and proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J. 2024;123:1449-1457 pubmed publisher
	Dao T, Rajendran A, Galagedera S, Haws W, Casta xf1 eda C. Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4. bioRxiv. 2023;: pubmed publisher
	McDowell I, Ayoubi R, Fotouhi M, Southern K, McPherson P, Laflamme C. The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence. F1000Res. 2023;12:355 pubmed publisher
	Buel G, Chen X, Myint W, Kayode O, Folimonova V, Cruz A, et al. E6AP AZUL interaction with UBQLN1/2 in cells, condensates, and an AlphaFold-NMR integrated structure. Structure. 2023;31:395-410.e6 pubmed publisher
	Phung T, Tatman M, Monteiro M. UBQLN2 undergoes a reversible temperature-induced conformational switch that regulates binding with HSPA1B: ALS/FTD mutations cripple the switch but do not destroy HSPA1B binding. Biochim Biophys Acta Gen Subj. 2023;1867:130284 pubmed publisher
	Lin B, Phung T, Higgins N, Greenslade J, Prado M, Finley D, et al. ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import. Hum Mol Genet. 2021;30:1230-1246 pubmed publisher
	Gerson J, Linton H, Xing J, Sutter A, Kakos F, Ryou J, et al. Shared and divergent phase separation and aggregation properties of brain-expressed ubiquilins. Sci Rep. 2021;11:287 pubmed publisher
	Whiteley A, Prado M, de Poot S, Paulo J, Ashton M, Domínguez S, et al. Global proteomics of Ubqln2-based murine models of ALS. J Biol Chem. 2021;296:100153 pubmed publisher
	Wang S, Tatman M, Monteiro M. Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD. Acta Neuropathol Commun. 2020;8:164 pubmed publisher
	Pickel C, Günter J, Ruiz Serrano A, Spielmann P, Fabrizio J, Wolski W, et al. Oxygen-dependent bond formation with FIH regulates the activity of the client protein OTUB1. Redox Biol. 2019;26:101265 pubmed publisher
	Alexander E, Ghanbari Niaki A, Zhang T, Sarkar J, Liu Y, Nirujogi R, et al. Ubiquilin 2 modulates ALS/FTD-linked FUS-RNA complex dynamics and stress granule formation. Proc Natl Acad Sci U S A. 2018;115:E11485-E11494 pubmed publisher
	Huang N, Erie C, Lu M, Wei J. Aberrant subcellular localization of SQSTM1/p62 contributes to increased vulnerability to proteotoxic stress recovery in Huntington's disease. Mol Cell Neurosci. 2018;88:43-52 pubmed publisher
	Teyssou E, Chartier L, Amador M, Lam R, Lautrette G, Nicol M, et al. Novel UBQLN2 mutations linked to amyotrophic lateral sclerosis and atypical hereditary spastic paraplegia phenotype through defective HSP70-mediated proteolysis. Neurobiol Aging. 2017;58:239.e11-239.e20 pubmed publisher
	Ma M, Hua S, Li G, Wang S, Cheng X, He S, et al. Prolyl hydroxylase domain protein 3 and asparaginyl hydroxylase factor inhibiting HIF-1 levels are predictive of tumoral behavior and prognosis in hepatocellular carcinoma. Oncotarget. 2017;8:12983-13002 pubmed publisher
	Le N, Chang L, Kovlyagina I, Georgiou P, Safren N, Braunstein K, et al. Motor neuron disease, TDP-43 pathology, and memory deficits in mice expressing ALS-FTD-linked UBQLN2 mutations. Proc Natl Acad Sci U S A. 2016;113:E7580-E7589 pubmed
	Hjerpe R, Bett J, Keuss M, Solovyova A, McWilliams T, Johnson C, et al. UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance by the Proteasome. Cell. 2016;166:935-949 pubmed publisher
	Mysore V, Szablowski J, Dervan P, FROST P. A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity. Mol Cancer Res. 2016;14:253-66 pubmed publisher
	Wollenick K, Hu J, Kristiansen G, Schraml P, Rehrauer H, Berchner Pfannschmidt U, et al. Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Nucleic Acids Res. 2012;40:1928-43 pubmed publisher
	Khan M, Bhattacharyya T, Andrikopoulos P, Esteban M, Barod R, Connor T, et al. Factor inhibiting HIF (FIH-1) promotes renal cancer cell survival by protecting cells from HIF-1?-mediated apoptosis. Br J Cancer. 2011;104:1151-9 pubmed publisher
	Yan M, Rayoo M, Takano E, Thorne H, Fox S. BRCA1 tumours correlate with a HIF-1alpha phenotype and have a poor prognosis through modulation of hydroxylase enzyme profile expression. Br J Cancer. 2009;101:1168-74 pubmed publisher
	Cockman M, Lancaster D, Stolze I, Hewitson K, McDonough M, Coleman M, et al. Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Proc Natl Acad Sci U S A. 2006;103:14767-72 pubmed
	Soilleux E, Turley H, Tian Y, Pugh C, Gatter K, Harris A. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology. 2005;47:602-10 pubmed
	Stolze I, Tian Y, Appelhoff R, Turley H, Wykoff C, Gleadle J, et al. Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected]. J Biol Chem. 2004;279:42719-25 pubmed

product information

master code :

NBP2-25164

SKU :

NBP2-25164

product name :

Ubiquilin 2 Antibody (6H9) - BSA Free

unit size :

0.1 ml (also 0.025 ml)

description :

The Ubiquilin 2 Antibody (6H9) - BSA Free from Novus is a mouse monoclonal antibody to Ubiquilin 2. This antibody reacts with human,mouse,rat. The Ubiquilin 2 Antibody (6H9) - BSA Free has been validated for the following applications: Immunocytochemistry/ Immunofluorescence,Western Blot,IF/IHC,Immunohistochemistry,Immunohistochemistry-Frozen,Immunoprecipitation,Simple Western,Knockout Validated,Knockdown Validated.

target :

Ubiquilin 2

category :

Primary Antibodies

buffer :

50% PBS, 50% glycerol

clonality :

Monoclonal

clone :

6H9

concentration :

1 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Human Ubiquilin 2 expressed in and purified from E. coli [UniProt# Q9UHD9]

isotype :

IgG1

purity :

Protein G purified

species :

Human,Mouse,Rat

theoretical molecular weight :

66-68 kDa

gene symbol :

UBQLN2

Antibody validation :

Knockout/Knockdown

accessionNumbers :

Q9UHD9

applications :

Western Blot,IF/IHC,Immunohistochemistry,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Immunoprecipitation,Simple Western,Knockout Validated,Knockdown Validated

USD :

399 USD

alt names :

Chap1, CHAP1/DSK2, DSK2, DSK2 homolog, FLJ10167, FLJ56541, hPLIC-2, N4BP4LIC-2, Nedd4 binding protein 4, PLIC-2, PLIC2Dsk2, Protein linking IAP with cytoskeleton 2, RIHFB2157, ubiquilin 2, ubiquilin-2, Ubiquitin-like product Chap1/Dsk2

storage :

Store at -20C. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage