product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Endophilin B1/Bif-1 Antibody (30A882.1.1) - BSA Free
catalog :
NBP2-24733
quantity :
0.1 mg (also 0.025 mg)
price :
409 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
30A882.1.1
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 46
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; loading ...; fig 7i
Cho C, Zeigler M, Mizuno S, Morrison R, Totah R, Barker Haliski M. Reductions in Hydrogen Sulfide and Changes in Mitochondrial Quality Control Proteins Are Evident in the Early Phases of the Corneally Kindled Mouse Model of Epilepsy. Int J Mol Sci. 2022;23: pubmed publisher
  • western blot; human; loading ...; fig 8b
Marcassa E, Raimondi M, Anwar T, Eskelinen E, Myers M, Triolo G, et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open. 2017;6:551-562 pubmed publisher
  • western blot; human
  • western blot; mouse
Wang D, Kinoshita Y, Kinoshita C, Uo T, Sopher B, Cudaback E, et al. Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain. 2015;138:2005-19 pubmed publisher
  • western blot; mouse
Dong H, Tian L, Li R, Pei C, Fu Y, Dong X, et al. IFNg-induced Irgm1 promotes tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy. Oncogene. 2015;34:5363-71 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Wang D, Uo T, Kinoshita C, Sopher B, Lee R, Murphy S, et al. Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons. J Neurosci. 2014;34:2674-83 pubmed publisher
  • immunocytochemistry; human
Cant J, Goodale M. Scratching beneath the surface: new insights into the functional properties of the lateral occipital area and parahippocampal place area. J Neurosci. 2011;31:8248-58 pubmed publisher
  • western blot; rat
Etxebarria A, Terrones O, Yamaguchi H, Landajuela A, Landeta O, Antonsson B, et al. Endophilin B1/Bif-1 stimulates BAX activation independently from its capacity to produce large scale membrane morphological rearrangements. J Biol Chem. 2009;284:4200-12 pubmed publisher
  • immunoprecipitation; human
Yamaguchi H, Woods N, Dorsey J, Takahashi Y, Gjertsen N, Yeatman T, et al. SRC directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis. J Biol Chem. 2008;283:19112-8 pubmed publisher
Hou P, Guo Y, Jin H, Sun J, Bai Y, Li W, et al. Bif-1c Attenuates Viral Proliferation by Regulating Autophagic Flux Blockade Induced by the Rabies Virus CVS-11 Strain in N2a Cells. Microbiol Spectr. 2023;11:e0307922 pubmed publisher
Frankowski H, Yeboah F, Berry B, Kinoshita C, Lee M, Evitts K, et al. Knock-Down of HDAC2 in Human Induced Pluripotent Stem Cell Derived Neurons Improves Neuronal Mitochondrial Dynamics, Neuronal Maturation and Reduces Amyloid Beta Peptides. Int J Mol Sci. 2021;22: pubmed publisher
Frangez Z, Seyed Jafari S, Hunger R, Simon H. Loss of Concurrent Regulation of the Expression of BIF-1, BAX, and Beclin-1 in Primary and Metastatic Melanoma. Biochemistry (Mosc). 2020;85:1227-1234 pubmed
Imperatore R, Tunisi L, Mavaro I, D angelo L, Attanasio C, Safari O, et al. Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice?. Animals (Basel). 2020;10: pubmed publisher
Frangez Z, Fernández Marrero Y, Stojkov D, Seyed Jafari S, Hunger R, Djonov V, et al. BIF-1 inhibits both mitochondrial and glycolytic ATP production: its downregulation promotes melanoma growth. Oncogene. 2020;39:4944-4955 pubmed publisher
Xu Y, Zhu Y, Bawa F, Hu S, Pan X, Yin L, et al. Hepatocyte-Specific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice. Hepatol Commun. 2020;4:527-539 pubmed publisher
Jo Y, Kim S, Garland K, Fuentes I, DiCarlo L, Ellis J, et al. Enhanced ER-associated degradation of HMG CoA reductase causes embryonic lethality associated with Ubiad1 deficiency. elife. 2020;9: pubmed publisher
Musante L, Bontha S, La Salvia S, Fernandez Piñeros A, Lannigan J, Le T, et al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs. Sci Rep. 2020;10:3701 pubmed publisher
Xu Y, Xu Y, Zhu Y, Sun H, Juguilon C, Li F, et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis. Mol Ther. 2020;28:202-216 pubmed publisher
Khalaj K, Miller J, Lingegowda H, Fazleabas A, Young S, Lessey B, et al. Extracellular vesicles from endometriosis patients are characterized by a unique miRNA-lncRNA signature. JCI Insight. 2019;4: pubmed publisher
Cho S, Xiao X, Wang S, Gao H, Rafikov R, Black S, et al. Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis. J Am Soc Nephrol. 2019;: pubmed publisher
Jo Y, Hamilton J, Hwang S, Garland K, Smith G, Su S, et al. Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice. elife. 2019;8: pubmed publisher
Tiedemann K, Sadvakassova G, Mikolajewicz N, Juhas M, Sabirova Z, Tabariès S, et al. Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis. Transl Oncol. 2019;12:462-474 pubmed publisher
Gao H, Guo H, Zhang H, Xie X, Wen P, Ren F. Yak-milk-derived exosomes promote proliferation of intestinal epithelial cells in an hypoxic environment. J Dairy Sci. 2019;102:985-996 pubmed publisher
Raimondi M, Cesselli D, Di Loreto C, La Marra F, Schneider C, Demarchi F. USP1 (ubiquitin specific peptidase 1) targets ULK1 and regulates its cellular compartmentalization and autophagy. Autophagy. 2019;15:613-630 pubmed publisher
Qi X, Schmiege P, Coutavas E, Li X. Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex. Science. 2018;362: pubmed publisher
Wang D, Kinoshita C, Kinoshita Y, Sopher B, Uo T, Lee R, et al. Neuronal susceptibility to beta-amyloid toxicity and ischemic injury involves histone deacetylase-2 regulation of endophilin-B1. Brain Pathol. 2019;29:164-175 pubmed publisher
Qi X, Schmiege P, Coutavas E, Wang J, Li X. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. 2018;560:128-132 pubmed publisher
Borgna V, Villegas J, Burzio V, Belmar S, Araya M, Jeldes E, et al. Mitochondrial ASncmtRNA-1 and ASncmtRNA-2 as potent targets to inhibit tumor growth and metastasis in the RenCa murine renal adenocarcinoma model. Oncotarget. 2017;8:43692-43708 pubmed publisher
Serfass J, Takahashi Y, Zhou Z, Kawasawa Y, Liu Y, Tsotakos N, et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection. J Biol Chem. 2017;292:10097-10111 pubmed publisher
Zhang C, Liu F, Chen H, Li N, Luo Z, Guo W, et al. Bif-1 promotes tumor cell migration and metastasis via Cdc42 expression and activity. Clin Exp Metastasis. 2017;34:11-23 pubmed publisher
Wild F, Khan M, Straka T, Rudolf R. Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo. Autophagy. 2016;12:2300-2310 pubmed
Xu Y, Li F, Zalzala M, Xu J, Gonzalez F, Adorini L, et al. Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice. Hepatology. 2016;64:1072-85 pubmed publisher
Li N, Subrahmanyan L, Smith E, Yu X, Zaidi S, Choi M, et al. Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus. Am J Hum Genet. 2016;98:1082-1091 pubmed publisher
Wang Y, Wang J, Wang Q, Wang Y, Guo C, Chen Q, et al. Endophilin B2 promotes inner mitochondrial membrane degradation by forming heterodimers with Endophilin B1 during mitophagy. Sci Rep. 2016;6:25153 pubmed publisher
Smagris E, Gilyard S, BasuRay S, Cohen J, Hobbs H. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem. 2016;291:10659-76 pubmed publisher
Takahashi Y, Tsotakos N, Liu Y, Young M, Serfass J, Tang Z, et al. The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs. Oncotarget. 2016;7:20855-68 pubmed publisher
Huynh N, VonMoss L, Smith D, Rahman I, Felemban M, Zuo J, et al. Characterization of Regulatory Extracellular Vesicles from Osteoclasts. J Dent Res. 2016;95:673-9 pubmed publisher
Xu S, Cherok E, Das S, Li S, Roelofs B, Ge S, et al. Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein. Mol Biol Cell. 2016;27:349-59 pubmed publisher
McFarlane M, Cantoria M, Linden A, January B, Liang G, Engelking L. Scap is required for sterol synthesis and crypt growth in intestinal mucosa. J Lipid Res. 2015;56:1560-71 pubmed publisher
Riemersma M, Sandrock J, Boltje T, Büll C, Heise T, Ashikov A, et al. Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid. Hum Mol Genet. 2015;24:2241-6 pubmed publisher
Runkle K, Meyerkord C, Desai N, Takahashi Y, Wang H. Bif-1 suppresses breast cancer cell migration by promoting EGFR endocytic degradation. Cancer Biol Ther. 2012;13:956-66 pubmed publisher
Takahashi Y, Meyerkord C, Hori T, Runkle K, Fox T, Kester M, et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 2011;7:61-73 pubmed
Yang J, Takahashi Y, Cheng E, Liu J, Terranova P, Zhao B, et al. GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death. J Cell Sci. 2010;123:861-70 pubmed publisher
Takahashi Y, Coppola D, Matsushita N, Cualing H, Sun M, Sato Y, et al. Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis. Nat Cell Biol. 2007;9:1142-51 pubmed
Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti S, et al. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol Cell Biol. 2005;25:9369-82 pubmed
Karbowski M, Jeong S, Youle R. Endophilin B1 is required for the maintenance of mitochondrial morphology. J Cell Biol. 2004;166:1027-39 pubmed
Cuddeback S, Yamaguchi H, Komatsu K, Miyashita T, Yamada M, Wu C, et al. Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax. J Biol Chem. 2001;276:20559-65 pubmed
product information
master code :
NBP2-24733
SKU :
NBP2-24733
product name :
Endophilin B1/Bif-1 Antibody (30A882.1.1) - BSA Free
unit size :
0.1 mg (also 0.025 mg)
description :
The Endophilin B1/Bif-1 Antibody (30A882.1.1) - BSA Free from Novus is a mouse monoclonal antibody to Endophilin B1/Bif-1. This antibody reacts with human,mouse,rat. The Endophilin B1/Bif-1 Antibody (30A882.1.1) - BSA Free has been validated for the following applications: IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Simple Western.
target :
Endophilin B1/Bif-1
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
30A882.1.1
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
A full-length human Bif-1 recombinant protein was used as immunogen.
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Human,Mouse,Rat
gene symbol :
SH3GLB1
accessionNumbers :
Q9Y371
applications :
IF/IHC,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Simple Western
USD :
409 USD
alt names :
Bax-interacting factor 1, Bif-1dJ612B15.2, CGI-61, endophilin-B1, KIAA0491endophilin B1, SH3 domain-containing GRB2-like protein B1, SH3-containing protein SH3GLB1, SH3-domain GRB2-like endophilin B1, SH3-domain, GRB2-like, endophilin B1
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.