product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
AMPK alpha 1 Antibody (2B7) - BSA Free
catalog :
NBP2-22127
quantity :
0.1 ml (also 0.025 ml)
price :
409 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2B7
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Novus Biologicals product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 5f
Xiao Y, Kwong M, Daemen A, Belvin M, Liang X, Hatzivassiliou G, et al. Metabolic Response to NAD Depletion across Cell Lines Is Highly Variable. PLoS ONE. 2016;11:e0164166 pubmed publisher
Mehanna E, Khalaf S, Mesbah N, Abo Elmatty D, Hafez M. Anti-oxidant, anti-apoptotic, and mitochondrial regulatory effects of selenium nanoparticles against vancomycin induced nephrotoxicity in experimental rats. Life Sci. 2022;288:120098 pubmed publisher
Nguyen K, Rivera A, Alzoubi M, Wathieu H, Dong S, Yousefi H, et al. Evaluation of liver kinase B1 downstream signaling expression in various breast cancers and relapse free survival after systemic chemotherapy treatment. Oncotarget. 2021;12:1110-1115 pubmed publisher
Jassim A, Cavanaugh M, Shah J, Willits R, Inman D. Transcorneal Electrical Stimulation Reduces Neurodegenerative Process in a Mouse Model of Glaucoma. Ann Biomed Eng. 2021;49:858-870 pubmed publisher
Harun Or Rashid M, Pappenhagen N, Zubricky R, Coughlin L, Jassim A, Inman D. MCT2 overexpression rescues metabolic vulnerability and protects retinal ganglion cells in two models of glaucoma. Neurobiol Dis. 2020;141:104944 pubmed publisher
Vila I, Park M, Setijono S, Yao Y, Kim H, Badin P, et al. A muscle-specific UBE2O/AMPKα2 axis promotes insulin resistance and metabolic syndrome in obesity. JCI Insight. 2019;4: pubmed publisher
Korponay T, Balnis J, Vincent C, Singer D, Chopra A, Adam A, et al. High CO2 downregulates skeletal muscle protein anabolism via AMPKα2-mediated depressed ribosomal biogenesis. Am J Respir Cell Mol Biol. 2019;: pubmed publisher
Harun Or Rashid M, Inman D. Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma. J Neuroinflammation. 2018;15:313 pubmed publisher
Chauhan A, Liu X, Jing J, Lee H, Yadav R, Liu J, et al. STIM2 interacts with AMPK and regulates calcium-induced AMPK activation. FASEB J. 2018;:fj201801225R pubmed publisher
Kotsarenko K, Lylo V, Ruban T, Macewicz L, Lukash L. Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro : Effect of Some Growth Factors and Cytokines. Biochem Genet. 2018;56:459-477 pubmed publisher
Dai S, Dulcey A, Hu X, Wassif C, Porter F, Austin C, et al. Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017;13:1435-1451 pubmed publisher
Altinoz M, Elmaci I, Bölükbaşı F, Ekmekci C, Yenmiş G, Sari R, et al. MGMT gene variants, temozolomide myelotoxicity and glioma risk. A concise literature survey including an illustrative case. J Chemother. 2017;29:238-244 pubmed publisher
Vila I, Yao Y, Kim G, Xia W, Kim H, Kim S, et al. A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy. Cancer Cell. 2017;31:208-224 pubmed publisher
Oktay Y, Ãœlgen E, Can Ã, Akyerli C, Yüksel Å, Erdemgil Y, et al. IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation. Sci Rep. 2016;6:27569 pubmed publisher
Stepanenko A, Andreieva S, Korets K, Mykytenko D, Baklaushev V, Huleyuk N, et al. Temozolomide promotes genomic and phenotypic changes in glioblastoma cells. Cancer Cell Int. 2016;16:36 pubmed publisher
Chen Y, Duan Y, Yang X, Sun L, Liu M, Wang Q, et al. Inhibition of ERK1/2 and activation of LXR synergistically reduce atherosclerotic lesions in ApoE-deficient mice. Arterioscler Thromb Vasc Biol. 2015;35:948-59 pubmed publisher
Fang Q, Inanç B, Schamus S, Wang X, Wei L, Brown A, et al. HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014;5:5513 pubmed publisher
Kewitz S, Stiefel M, Kramm C, Staege M. Impact of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and MGMT expression on dacarbazine resistance of Hodgkin's lymphoma cells. Leuk Res. 2014;38:138-43 pubmed publisher
Persano L, Pistollato F, Rampazzo E, Della Puppa A, Abbadi S, Frasson C, et al. BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1? stability and MGMT expression. Cell Death Dis. 2012;3:e412 pubmed publisher
Lylo V, Matsevich L, Kotsarenko E, Babenko L, Korneliuk A, Sukhorada E, et al. [Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]. Tsitol Genet. 2011;45:53-60 pubmed
Herzig M, Hildreth K, Huamani J, Perez M, Goins B, McMahan C, et al. Human O6 -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Mol Carcinog. 2013;52:275-85 pubmed publisher
Della Puppa A, Persano L, Masi G, Rampazzo E, Sinigaglia A, Pistollato F, et al. MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?. J Neurooncol. 2012;106:33-41 pubmed publisher
Tang J, Svilar D, Trivedi R, Wang X, Goellner E, Moore B, et al. N-methylpurine DNA glycosylase and DNA polymerase beta modulate BER inhibitor potentiation of glioma cells to temozolomide. Neuro Oncol. 2011;13:471-86 pubmed publisher
Pistollato F, Abbadi S, Rampazzo E, Persano L, Della Puppa A, Frasson C, et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells. 2010;28:851-62 pubmed publisher
Cai S, Xu Y, Cooper R, Ferkowicz M, Hartwell J, Pollok K, et al. Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents. Cancer Res. 2005;65:3319-27 pubmed
Zuo C, Ai L, Ratliff P, Suen J, Hanna E, Brent T, et al. O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 2004;13:967-75 pubmed
Roth T, Xu Y, Luo M, Kelley M. Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection. Cancer Gene Ther. 2003;10:603-10 pubmed
Liu L, Spiro T, Qin X, Majka S, Haaga J, Schupp J, et al. Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine. Clin Cancer Res. 2001;7:2318-24 pubmed
product information
brand :
Novus
catalog number base :
NBP2-22127
SKU :
NBP2-22127
product name :
AMPK alpha 1 Antibody (2B7) - BSA Free
units size :
0.1 ml (also 0.025 ml)
description :
The AMPK alpha 1 Antibody (2B7) - BSA Free from Novus is a mouse monoclonal antibody to AMPK alpha 1. This antibody reacts with human,mouse,primate,rat. The AMPK alpha 1 Antibody (2B7) - BSA Free has been validated for the following applications: Flow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry/ Immunofluorescence.
target :
AMPK alpha 1
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
2B7
concentration :
1.0 mg/ml
conjugate :
Unconjugated
all_available conjugate :
Unconjugated
host :
Mouse
immunogen :
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
isotype :
IgG1
purity :
Protein A or G purified
species :
Human,Mouse,Primate,Rat
specificity :
Detects endogenous levels of AMPK alpha 1 only when phosphorylated at serine 496
theoretical molecular weight :
64 kDa
gene symbol :
PRKAA1
Antibody validation :
Biological Validation
top caption :
Simple Western: AMPK alpha 1 Antibody (2B7)BSA Free [NBP2-22127]
accessionNumbers :
Q13131
applications :
Flow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry/ Immunofluorescence
USD :
409 USD
alt names :
AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK, AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain, AMPK subunit alpha-1, AMPK1, AMPKa1, EC 2.7.11, EC 2.7.11.1, MGC33776, MGC57364, protein kinase, AMP-activated, alpha 1 catalytic subunit
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.