product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
iNOS Antibody (4E5) - BSA Free
catalog :
NBP2-22119
quantity :
0.1 ml (also 0.025 ml)
price :
429 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4.00E+05
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3h
  • flow cytometry; human; 1:100; loading ...; fig s8a
Tyagi A, Sharma S, Wu K, Wu S, Xing F, Liu Y, et al. Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung. Nat Commun. 2021;12:474 pubmed publisher
  • immunohistochemistry - paraffin section; human
Wang Y, Wang W, Zhang Y, Zhao X, Yang D. Clinical significance of a myeloperoxidase gene polymorphism and inducible nitric oxide synthase expression in cirrhotic patients with hepatopulmonary syndrome. J Huazhong Univ Sci Technolog Med Sci. 2010;30:437-42 pubmed publisher
Beielstein A, Izquierdo E, Blakemore S, Nickel N, Michalik M, Chawan S, et al. Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibition. Cell Rep Med. 2024;5:101830 pubmed publisher
Middelburg J, Ghaffari S, Schoufour T, Sluijter M, Schaap G, G xf6 yn xfc k B, et al. The MHC-E peptide ligands for checkpoint CD94/NKG2A are governed by inflammatory signals, whereas LILRB1/2 receptors are peptide indifferent. Cell Rep. 2023;42:113516 pubmed publisher
He Z, Greven J, Shi Y, Qin K, Zhao Q, Zhang X, et al. Extracellular vesicles derived from endothelial cells modulate macrophage phenotype in vitro. Eur J Med Res. 2023;28:506 pubmed publisher
Zhang Y, Li W, Guo S, Wu Z, Zhang L, Liu Y, et al. FBXO22 Mediates the NGF/TRKA Signaling Pathway in Bone Metastases in Prostate Cancer. Am J Pathol. 2023;193:1248-1266 pubmed publisher
You M, Song Y, Chen J, Liu Y, Chen W, Cen Y, et al. Combined exposure to benzo(a)pyrene and dibutyl phthalate aggravates pro-inflammatory macrophage polarization in spleen via pyroptosis involving cathepsin B. Sci Total Environ. 2023;881:163460 pubmed publisher
Zhao S, Zhou L, Wang Q, Cao J, Chen Y, Wang W, et al. Elevated branched-chain amino acid promotes atherosclerosis progression by enhancing mitochondrial-to-nuclear H2O2-disulfide HMGB1 in macrophages. Redox Biol. 2023;62:102696 pubmed publisher
Kong Y, Shi W, Zheng L, Zhang D, Jiang X, Liu B, et al. In situ delivery of a curcumin-loaded dynamic hydrogel for the treatment of chronic peripheral neuropathy. J Control Release. 2023;357:319-332 pubmed publisher
Zheng X, Chen L, Tan J, Miao J, Liu X, Yang T, et al. Effect of micro/nano-sheet array structures on the osteo-immunomodulation of macrophages. Regen Biomater. 2022;9:rbac075 pubmed publisher
Wilkinson H, Reubinoff B, Shveiky D, Hardman M, Menachem Zidon O. Epithelial arginase-1 is a key mediator of age-associated delayed healing in vaginal injury. Front Endocrinol (Lausanne). 2022;13:927224 pubmed publisher
Zhang W, Chen J, Guo W, Kong G, Wang L, Cheng X, et al. WKYMVm/FPR2 Alleviates Spinal Cord Injury by Attenuating the Inflammatory Response of Microglia. Mediators Inflamm. 2022;2022:4408099 pubmed publisher
Chen Y, Li P, Zhen R, Wang L, Feng J, Xie Y, et al. Effects of niacin on intestinal epithelial Barrier, intestinal Immunity, and microbial community in weaned piglets challenged by PDCoV. Int Immunopharmacol. 2022;111:109054 pubmed publisher
Yan B, Tan J, Zhang H, Liu L, Chen L, Qiao Y, et al. Constructing fluorine-doped Zr-MOF films on titanium for antibacteria, anti-inflammation, and osteogenesis. Biomater Adv. 2022;134:112699 pubmed publisher
Ganz T, Fainstein N, Elad A, Lachish M, Goldfarb S, Einstein O, et al. Microbial pathogens induce neurodegeneration in Alzheimer's disease mice: protection by microglial regulation. J Neuroinflammation. 2022;19:5 pubmed publisher
Roy R, Zayas J, Mohamed M, Aboonabi A, Delgado K, Wallace J, et al. IL-10 Dysregulation Underlies Chemokine Insufficiency, Delayed Macrophage Response, and Impaired Healing in Diabetic Wounds. J Invest Dermatol. 2022;142:692-704.e14 pubmed publisher
Fan P, Yang Y, Liu T, Lu X, Huang H, Chen L, et al. Anti-atopic effect of Viola yedoensis ethanol extract against 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin dysfunction. J Ethnopharmacol. 2021;280:114474 pubmed publisher
Shueng P, Yu L, Chiu H, Chang H, Chiu Y, Kuo T, et al. Early phago-/endosomal escape of platinum drugs via ROS-responsive micelles for dual cancer chemo/immunotherapy. Biomaterials. 2021;276:121012 pubmed publisher
Marques R, Gonzalez Nuñez M, Walker M, Gómez E, Colas R, Montero Melendez T, et al. Loss of 15-lipoxygenase disrupts Treg differentiation altering their pro-resolving functions. Cell Death Differ. 2021;28:3140-3160 pubmed publisher
Hu C, Chu C, Liu L, Wang C, Jin S, Yang R, et al. Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing. Sci Adv. 2021;7: pubmed publisher
Cui J, Shan K, Yang Q, Qi Y, Qu H, Li J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021;25:5586-5601 pubmed publisher
Selvaraj U, Ujas T, Kong X, Kumar A, Plautz E, Zhang S, et al. Delayed diapedesis of CD8 T cells contributes to long-term pathology after ischemic stroke in male mice. Brain Behav Immun. 2021;95:502-513 pubmed publisher
Zhang J, Thakuri B, Zhao J, Nguyen L, Nguyen L, Khanal S, et al. Long Noncoding RNA RUNXOR Promotes Myeloid-Derived Suppressor Cell Expansion and Functions via Enhancing Immunosuppressive Molecule Expressions during Latent HIV Infection. J Immunol. 2021;206:2052-2060 pubmed publisher
Hu G, Su Y, Kang B, Fan Z, Dong T, Brown D, et al. High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming. Nat Commun. 2021;12:773 pubmed publisher
Martin Cordero L, Galvez I, Hinchado M, Ortega E. Influence of Obesity and Exercise on β2-Adrenergic-Mediated Anti-Inflammatory Effects in Peritoneal Murine Macrophages. Biomedicines. 2020;8: pubmed publisher
Su S, Qin S, Xian X, Huang F, Huang Q, ZhangDi H, et al. Interleukin-22 regulating Kupffer cell polarization through STAT3/Erk/Akt crosstalk pathways to extenuate liver fibrosis. Life Sci. 2021;264:118677 pubmed publisher
Tan H, Wang N, Zhang C, Chan Y, Yuen M, Feng Y. Lysyl Oxidase-Like 4 Fosters an Immunosuppressive Microenvironment During Hepatocarcinogenesis. Hepatology. 2021;73:2326-2341 pubmed publisher
Feng J, Chen S, Li S, Wu B, Lu J, Tan L, et al. The association between monocytic myeloid-derived suppressor cells levels and the anti-tumor efficacy of anti-PD-1 therapy in NSCLC patients. Transl Oncol. 2020;13:100865 pubmed publisher
Goldfarb S, Fainstein N, Ben Hur T. Electroconvulsive stimulation attenuates chronic neuroinflammation. JCI Insight. 2020;5: pubmed publisher
Fukuyama K, Fukuzawa M, Okubo R, Okada M. Upregulated Connexin 43 Induced by Loss-of-Functional S284L-Mutant α4 Subunit of Nicotinic ACh Receptor Contributes to Pathomechanisms of Autosomal Dominant Sleep-Related Hypermotor Epilepsy. Pharmaceuticals (Basel). 2020;13: pubmed publisher
Fukuyama K, Okubo R, Murata M, Shiroyama T, Okada M. Activation of Astroglial Connexin is Involved in Concentration-Dependent Double-Edged Sword Clinical Action of Clozapine. Cells. 2020;9: pubmed publisher
Fukuyama K, Fukuzawa M, Shiroyama T, Okada M. Pathogenesis and pathophysiology of autosomal dominant sleep-related hypermotor epilepsy with S284L-mutant α4 subunit of nicotinic ACh receptor. Br J Pharmacol. 2020;177:2143-2162 pubmed publisher
Nishri Y, Hampton D, Ben Shushan E, Fainstein N, Magnani D, Aharonowiz M, et al. Continuous Immune-Modulatory Effects of Human Olig2+ Precursor Cells Attenuating a Chronic-Active Model of Multiple Sclerosis. Mol Neurobiol. 2020;57:1021-1034 pubmed publisher
Smith H, Hirukawa A, Sanguin Gendreau V, Nandi I, Dufour C, Zuo D, et al. An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis. Nat Commun. 2019;10:2901 pubmed publisher
Galvez I, Martin Cordero L, Hinchado M, Alvarez Barrientos A, Ortega E. Anti-inflammatory effect of β2 adrenergic stimulation on circulating monocytes with a pro-inflammatory state in high-fat diet-induced obesity. Brain Behav Immun. 2019;: pubmed publisher
Bastian C, Zaleski J, Stahon K, Parr B, McCray A, Day J, et al. NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels. J Neurosci. 2018;38:6247-6266 pubmed publisher
Pistorius K, Souza P, De Matteis R, Austin Williams S, Primdahl K, Vik A, et al. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function. Cell Chem Biol. 2018;25:749-760.e9 pubmed publisher
Omlor A, Le D, Schlicker J, Hannig M, Ewen R, Heck S, et al. Local Effects on Airway Inflammation and Systemic Uptake of 5 nm PEGylated and Citrated Gold Nanoparticles in Asthmatic Mice. Small. 2017;13: pubmed publisher
Rauen T, Frye B, Wang J, Raffetseder U, Alidousty C, En Nia A, et al. Cold shock protein YB-1 is involved in hypoxia-dependent gene transcription. Biochem Biophys Res Commun. 2016;478:982-7 pubmed publisher
Duprey Díaz M, Blagburn J, Blanco R. Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system. J Chem Neuroanat. 2016;77:80-92 pubmed publisher
Wu J, Liu J, Ko Y, Wang C, Chung Y, Chu K, et al. Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy. Hum Mol Genet. 2016;25:1637-47 pubmed publisher
Okuyama N, Matsuda S, Yamashita A, Moriguchi Goto S, Sameshima N, Iwakiri T, et al. Human coronary thrombus formation is associated with degree of plaque disruption and expression of tissue factor and hexokinase II. Circ J. 2015;79:2430-8 pubmed publisher
Padgett L, Burg A, Lei W, Tse H. Loss of NADPH oxidase-derived superoxide skews macrophage phenotypes to delay type 1 diabetes. Diabetes. 2015;64:937-46 pubmed publisher
product information
master code :
NBP2-22119
SKU :
NBP2-22119
product name :
iNOS Antibody (4E5) - BSA Free
unit size :
0.1 ml (also 0.025 ml)
description :
The iNOS Antibody (4E5) - BSA Free from Novus is a mouse monoclonal antibody to iNOS. This antibody reacts with human,mouse,rat. The iNOS Antibody (4E5) - BSA Free has been validated for the following applications: Immunohistochemistry-Paraffin,Flow Cytometry,In vivo assay,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,ELISA,Flow (Intracellular).
target :
iNOS
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
4.00E+05
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
iNOS Antibody (4E5) was made to a purified recombinant fragment of human iNOS (C-terminus) expressed in E. coli. [UniProt# P35228]
isotype :
IgG1
purity :
Protein A purified
species :
Human,Mouse,Rat
theoretical molecular weight :
131 kDa
gene symbol :
NOS2
accessionNumbers :
P35228
applications :
Flow Cytometry,In vivo assay,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,ELISA,Flow (Intracellular)
USD :
429 USD
alt names :
EC 1.14.13.39, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, INOS, nitric oxide synthase 2, inducible, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, inducible, nitric oxide synthase, macrophage, NOS, NOS type II, NOS2A, Peptidyl-cysteine S-nitrosylase NOS2
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.