product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
RIPK3/RIP3 Antibody - BSA Free
catalog :
NBP1-77299
quantity :
0.1 mg (also 0.025 mg)
price :
499 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, western blot knockout validation
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:2000; fig 5
Gautheron J, Vucur M, Schneider A, Severi I, Roderburg C, Roy S, et al. The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance. Nat Commun. 2016;7:11869 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3b
Li Y, Li Z, Quan Y, Cheng H, Riquelme M, Li X, et al. Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis. iScience. 2021;24:102533 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 5f
  • western blot; rat; 1:1000; loading ...; fig 2e
Cheng J, Duan X, Fu X, Jiang Y, Yang P, Cao C, et al. RIP1 Perturbation Induces Chondrocyte Necroptosis and Promotes Osteoarthritis Pathogenesis via Targeting BMP7. Front Cell Dev Biol. 2021;9:638382 pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig s3g
Newton K, Wickliffe K, Maltzman A, Dugger D, Strasser A, Pham V, et al. RIPK1 inhibits ZBP1-driven necroptosis during development. Nature. 2016;540:129-133 pubmed publisher
Nam Y, Shin J, Kim S, Hwang C, Lee C, Hwang G, et al. EGFR inhibits TNF-α-mediated pathway by phosphorylating TNFR1 at tyrosine 360 and 401. Cell Death Differ. 2024;31:1318-1332 pubmed publisher
Jia Y, Cheng L, Yang J, Mao J, Xie Y, Yang X, et al. miR-223-3p Prevents Necroptotic Macrophage Death by Targeting Ripk3 in a Negative Feedback Loop and Consequently Ameliorates Advanced Atherosclerosis. Arterioscler Thromb Vasc Biol. 2024;44:218-237 pubmed publisher
Selvarani R, Van Michelle Nguyen H, Thadathil N, Wolf R, Freeman W, Wiley C, et al. Characterization of novel mouse models to study the role of necroptosis in aging and age-related diseases. GeroScience. 2023;45:3241-3256 pubmed publisher
Taraborrelli L, x15e enbabao x11f lu Y, Wang L, Lim J, Blake K, Kljavin N, et al. Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer. Nat Commun. 2023;14:5945 pubmed publisher
Zhou H, Zhou L, Guan Q, Hou X, Wang C, Liu L, et al. Skp2-mediated MLKL degradation confers cisplatin-resistant in non-small cell lung cancer cells. Commun Biol. 2023;6:805 pubmed publisher
Wen H, Chen J, Zhao B, Wang S, Ma A, Dong H, et al. Repression of ferroptotic cell death by mitochondrial calcium signaling. Res Sq. 2023;: pubmed publisher
Chen X, Huang L, Tang J, Wu D, An N, Ye Z, et al. Asiatic acid alleviates cisplatin-induced renal fibrosis in tumor-bearing mice by improving the TFEB-mediated autophagy-lysosome pathway. Biomed Pharmacother. 2023;165:115122 pubmed publisher
Mohammed S, Thadathil N, Ohene Marfo P, Tran A, Van Der Veldt M, Georgescu C, et al. Absence of Either Ripk3 or Mlkl Reduces Incidence of Hepatocellular Carcinoma Independent of Liver Fibrosis. Mol Cancer Res. 2023;21:933-946 pubmed publisher
Lyons J, Mandal P, Otani S, Chihade D, Easley K, Swift D, et al. The RIPK3 Scaffold Regulates Lung Inflammation During Pseudomonas Aeruginosa Pneumonia. Am J Respir Cell Mol Biol. 2023;68:150-160 pubmed publisher
Yan W, Zhao W, Hu X, Ban X, Ning W, Wan H, et al. PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons. Neural Regen Res. 2023;18:357-363 pubmed publisher
Du Y, Zhong Y, Ding R, Wang X, Xia F, Zhang Q, et al. New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice. Front Cell Infect Microbiol. 2022;12:1068324 pubmed publisher
Hwang S, Ha Y, Koo G, Noh H, Lee A, Kim B, et al. LCK-Mediated RIPK3 Activation Controls Double-Positive Thymocyte Proliferation and Restrains Thymic Lymphoma by Regulating the PP2A-ERK Axis. Adv Sci (Weinh). 2022;9:e2204522 pubmed publisher
Dong Y, Yu H, Li X, Bian K, Zheng Y, Dai M, et al. Hyperphosphorylated tau mediates neuronal death by inducing necroptosis and inflammation in Alzheimer's disease. J Neuroinflammation. 2022;19:205 pubmed publisher
Cacciola N, Salzano A, D Onofrio N, Venneri T, Cicco P, Vinale F, et al. Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer. Int J Mol Sci. 2022;23: pubmed publisher
Abd El Aal S, Abdelrahman M, Reda A, Afify H, Ragab G, El Gazar A, et al. Galangin mitigates DOX-induced cognitive impairment in rats: Implication of NOX-1/Nrf-2/HMGB1/TLR4 and TNF-α/MAPKs/RIPK/MLKL/BDNF. Neurotoxicology. 2022;92:77-90 pubmed publisher
Feng Y, Imam Aliagan A, Tombo N, Draeger D, Bopassa J. RIP3 Translocation into Mitochondria Promotes Mitofilin Degradation to Increase Inflammation and Kidney Injury after Renal Ischemia-Reperfusion. Cells. 2022;11: pubmed publisher
Lee J, Lee S, Min S, Kang S. RIP3-Dependent Accumulation of Mitochondrial Superoxide Anions in TNF-α-Induced Necroptosis. Mol Cells. 2022;45:193-201 pubmed publisher
Parshenkov A, Hennet T. Glycosylation-Dependent Induction of Programmed Cell Death in Murine Adenocarcinoma Cells. Front Immunol. 2022;13:797759 pubmed publisher
D Onofrio N, Martino E, Balestrieri A, Mele L, Cautela D, Castaldo D, et al. Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway. FEBS Lett. 2022;596:1313-1329 pubmed publisher
Shi S, Verstegen M, Roest H, Ardisasmita A, Cao W, Roos F, et al. Recapitulating Cholangiopathy-Associated Necroptotic Cell Death In Vitro Using Human Cholangiocyte Organoids. Cell Mol Gastroenterol Hepatol. 2022;13:541-564 pubmed publisher
Rudalska R, Harbig J, Snaebjornsson M, Klotz S, Zwirner S, Taranets L, et al. LXRα activation and Raf inhibition trigger lethal lipotoxicity in liver cancer. Nat Cancer. 2021;2:201-217 pubmed publisher
Peters E, Karver M, Sun K, Gillis D, Biswas S, Clemons T, et al. Self-Assembled Peptide Amphiphile Nanofibers for Controlled Therapeutic Delivery to the Atherosclerotic Niche. Adv Ther (Weinh). 2021;4: pubmed publisher
Zou J, Shi Q, Chen H, Juskevicius R, Zinkel S. Programmed necroptosis is upregulated in low-grade myelodysplastic syndromes and may play a role in the pathogenesis. Exp Hematol. 2021;103:60-72.e5 pubmed publisher
Thadathil N, Nicklas E, Mohammed S, Lewis T, Richardson A, Deepa S. Necroptosis increases with age in the brain and contributes to age-related neuroinflammation. GeroScience. 2021;43:2345-2361 pubmed publisher
Fu B, Lin X, Tan S, Zhang R, Xue W, Zhang H, et al. MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death. EMBO Rep. 2021;22:e52252 pubmed publisher
Byun H, Kwon S, Wagner K, Shin H, Lim H. Tumor susceptibility gene 101 is required for the maintenance of uterine epithelial cells during embryo implantation. Reprod Biol Endocrinol. 2021;19:112 pubmed publisher
Xu H, Cai Y, Yu M, Sun J, Cai J, Li J, et al. Celastrol protects against early brain injury after subarachnoid hemorrhage in rats through alleviating blood-brain barrier disruption and blocking necroptosis. Aging (Albany NY). 2021;13:16816-16833 pubmed publisher
Garcia L, Tenev T, Newman R, Haich R, Liccardi G, John S, et al. Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance. Nat Commun. 2021;12:3364 pubmed publisher
Gao S, Griffin C. RIPK3 modulates growth factor receptor expression in endothelial cells to support angiogenesis. Angiogenesis. 2021;: pubmed publisher
Baidya R, Gautheron J, Crawford D, Wang H, Bridle K. Inhibition of MLKL Attenuates Necroptotic Cell Death in a Murine Cell Model of Ischaemia Injury. J Clin Med. 2021;10: pubmed publisher
Dai Q, Zhang Y, Liao X, Jiang Y, Lv X, Yuan X, et al. Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway. Front Pharmacol. 2020;11:534775 pubmed publisher
Zhou H, Liu L, Ma X, Wang J, Yang J, Zhou X, et al. RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage. Mol Cell Biochem. 2021;476:1233-1243 pubmed publisher
LingHu H, Luo H, Gang L. Bufalin Induces Glioma Cell Death by Apoptosis or Necroptosis. Onco Targets Ther. 2020;13:4767-4778 pubmed publisher
Seo J, Seong D, Nam Y, Hwang C, Lee S, Lee C, et al. Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex. Cell Death Differ. 2020;: pubmed publisher
Lyu A, Kim T, Park S, Shin S, Jeong S, Yu Y, et al. Mitochondrial Damage and Necroptosis in Aging Cochlea. Int J Mol Sci. 2020;21: pubmed publisher
Zhou H, Xu C, Lee H, Yoon Y, Chen W. Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing. Mol Metab. 2020;36:100971 pubmed publisher
Colijn S, Muthukumar V, Xie J, Gao S, Griffin C. Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis. Dis Model Mech. 2020;13: pubmed publisher
Popper B, Rammer M, Gasparitsch M, Singer T, Keller U, Döring Y, et al. Neonatal obstructive nephropathy induces necroptosis and necroinflammation. Sci Rep. 2019;9:18600 pubmed publisher
Du X, Ge W, Jing R, Pan L. Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1. Int Immunopharmacol. 2019;77:105944 pubmed publisher
Takezaki A, Tsukumo S, Setoguchi Y, Ledford J, Goto H, Hosomichi K, et al. A homozygous SFTPA1 mutation drives necroptosis of type II alveolar epithelial cells in patients with idiopathic pulmonary fibrosis. J Exp Med. 2019;216:2724-2735 pubmed publisher
Orozco S, Daniels B, Yatim N, Messmer M, Quarato G, Chen Harris H, et al. RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity. Cell Rep. 2019;28:2275-2287.e5 pubmed publisher
Chen J, Jin H, Xu H, Peng Y, Jie L, Xu D, et al. The Neuroprotective Effects of Necrostatin-1 on Subarachnoid Hemorrhage in Rats Are Possibly Mediated by Preventing Blood-Brain Barrier Disruption and RIP3-Mediated Necroptosis. Cell Transplant. 2019;28:1358-1372 pubmed publisher
Lim J, Park H, Heisler J, Maculins T, Roose Girma M, Xu M, et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. elife. 2019;8: pubmed publisher
Lloyd A, Davies C, Holloway R, Labrak Y, Ireland G, Carradori D, et al. Central nervous system regeneration is driven by microglia necroptosis and repopulation. Nat Neurosci. 2019;22:1046-1052 pubmed publisher
Font Belmonte E, Ugidos I, Santos Galdiano M, Gonzalez Rodriguez P, Anuncibay Soto B, Pérez Rodríguez D, et al. Post-ischemic salubrinal administration reduces necroptosis in a rat model of global cerebral ischemia. J Neurochem. 2019;: pubmed publisher
Li X, Gong W, Wang H, Li T, Attri K, Lewis R, et al. O-GlcNAc Transferase Suppresses Inflammation and Necroptosis by Targeting Receptor-Interacting Serine/Threonine-Protein Kinase 3. Immunity. 2019;50:576-590.e6 pubmed publisher
Wu Z, Fournel L, Stadler N, Liu J, Boullier A, Hoyeau N, et al. Modulation of lung cancer cell plasticity and heterogeneity with the restoration of cisplatin sensitivity by neurotensin antibody. Cancer Lett. 2019;444:147-161 pubmed publisher
Hernandez D, Salvadores N, Moya Alvarado G, Catalán R, Bronfman F, Court F. Axonal degeneration induced by glutamate excitotoxicity is mediated by necroptosis. J Cell Sci. 2018;131: pubmed publisher
Hou H, Wang Y, Li Q, Li Z, Teng Y, Li J, et al. The role of RIP3 in cardiomyocyte necrosis induced by mitochondrial damage of myocardial ischemia-reperfusion. Acta Biochim Biophys Sin (Shanghai). 2018;50:1131-1140 pubmed publisher
Rodriguez D, Green D. Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis. Methods Mol Biol. 2018;1857:71-83 pubmed publisher
Chen T, Pan H, Li J, Xu H, Jin H, Qian C, et al. Inhibiting of RIPK3 attenuates early brain injury following subarachnoid hemorrhage: Possibly through alleviating necroptosis. Biomed Pharmacother. 2018;107:563-570 pubmed publisher
Li P, Zhou Y, Goodwin A, Cook J, Halushka P, Zhang X, et al. Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis. J Infect Dis. 2018;218:1995-2005 pubmed publisher
Zamaraev A, Kopeina G, Buchbinder J, Zhivotovsky B, Lavrik I. Caspase-2 is a negative regulator of necroptosis. Int J Biochem Cell Biol. 2018;102:101-108 pubmed publisher
Piao X, Miura R, Miyake S, Komazawa Sakon S, Koike M, Shindo R, et al. Blockade of TNF receptor superfamily 1 (TNFR1)-dependent and TNFR1-independent cell death is crucial for normal epidermal differentiation. J Allergy Clin Immunol. 2019;143:213-228.e10 pubmed publisher
Feldmann F, Schenk B, Martens S, Vandenabeele P, Fulda S. Sorafenib inhibits therapeutic induction of necroptosis in acute leukemia cells. Oncotarget. 2017;8:68208-68220 pubmed publisher
Martens S, Jeong M, Tonnus W, Feldmann F, Hofmans S, Goossens V, et al. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury. Cell Death Dis. 2017;8:e2904 pubmed publisher
Sridharan H, Ragan K, Guo H, Gilley R, Landsteiner V, Kaiser W, et al. Murine cytomegalovirus IE3-dependent transcription is required for DAI/ZBP1-mediated necroptosis. EMBO Rep. 2017;18:1429-1441 pubmed publisher
Gong Y, Guy C, Olauson H, Becker J, Yang M, Fitzgerald P, et al. ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences. Cell. 2017;169:286-300.e16 pubmed publisher
Ros U, Peña Blanco A, Hänggi K, Kunzendorf U, Krautwald S, Wong W, et al. Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium. Cell Rep. 2017;19:175-187 pubmed publisher
Yang S, Lee D, Shin J, Lee S, Baek S, Kim J, et al. Nec-1 alleviates cognitive impairment with reduction of Aβ and tau abnormalities in APP/PS1 mice. EMBO Mol Med. 2017;9:61-77 pubmed publisher
Shindo R, Yamazaki S, Ohmuraya M, Araki K, Nakano H. Short form FLICE-inhibitory protein promotes TNF?-induced necroptosis in fibroblasts derived from CFLARs transgenic mice. Biochem Biophys Res Commun. 2016;480:23-28 pubmed publisher
Qin D, Wang X, Li Y, Yang L, Wang R, Peng J, et al. MicroRNA-223-5p and -3p Cooperatively Suppress Necroptosis in Ischemic/Reperfused Hearts. J Biol Chem. 2016;291:20247-59 pubmed publisher
Lloyd A, Miron V. Cellular and Molecular Mechanisms Underpinning Macrophage Activation during Remyelination. Front Cell Dev Biol. 2016;4:60 pubmed publisher
Seo J, Lee E, Sung H, Seong D, Dondelinger Y, Shin J, et al. CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3. Nat Cell Biol. 2016;18:291-302 pubmed publisher
Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, et al. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Nature. 2015;528:370-5 pubmed publisher
Yoon S, Bogdanov K, Kovalenko A, Wallach D. Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it. Cell Death Differ. 2016;23:253-60 pubmed publisher
Miki Y, Akimoto J, Moritake K, Hironaka C, Fujiwara Y. Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells. Lasers Med Sci. 2015;30:1739-45 pubmed publisher
Dara L, Johnson H, Suda J, Win S, Gaarde W, Han D, et al. Receptor interacting protein kinase 1 mediates murine acetaminophen toxicity independent of the necrosome and not through necroptosis. Hepatology. 2015;62:1847-57 pubmed publisher
Preyat N, Rossi M, Kers J, Chen L, Bertin J, Gough P, et al. Intracellular nicotinamide adenine dinucleotide promotes TNF-induced necroptosis in a sirtuin-dependent manner. Cell Death Differ. 2016;23:29-40 pubmed publisher
product information
master code :
NBP1-77299
SKU :
NBP1-77299
product name :
RIPK3/RIP3 Antibody - BSA Free
unit size :
0.1 mg (also 0.025 mg)
description :
The RIPK3/RIP3 Antibody - BSA Free from Novus is a rabbit polyclonal antibody to RIPK3/RIP3. This antibody reacts with human,mouse,rat. The RIPK3/RIP3 Antibody - BSA Free has been validated for the following applications: Western Blot,Simple Western,IF/IHC,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Proximity Ligation Assay,Gel Supershift Assay,Knockout Validated,Knockdown Validated,Immunocytochemistry/ Immunofluorescence,ELISA.
target :
RIPK3/RIP3
category :
Primary Antibodies
buffer :
PBS
clonality :
Polyclonal
concentration :
1 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
RIPK3/RIP3 Antibody was made to a 14 amino acid peptide near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3. Amino Acid Squence: AQFGRGRGWQPFHK
isotype :
IgG
purity :
Peptide affinity purified
species :
Human,Mouse,Rat
specificity :
Mouse RIPK3/RIP3 Antibody has one isoform (486aa, 53 kDa)
theoretical molecular weight :
53 kDa
gene symbol :
RIPK3
Antibody validation :
Knockout/Knockdown
accessionNumbers :
AAF03133
applications :
Western Blot,Simple Western,IF/IHC,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Proximity Ligation Assay,Gel Supershift Assay,Knockout Validated,Knockdown Validated,Immunocytochemistry/ Immunofluorescence,ELISA
USD :
499 USD
alt names :
EC 2.7.11.1, Receptor Interacting Serine/Threonine Kinase 3, Receptor-interacting protein 3, receptor-interacting serine/threonine-protein kinase 3, receptor-interacting serine-threonine kinase 3, RIP3, RIP-3, RIP-like protein kinase 3
storage :
Store at 4C.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.