PKM2 Antibody - BSA Free | Novus Biologicals NBP1-48308 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

PKM2 Antibody - BSA Free

catalog :

NBP1-48308

quantity :

0.1 ml (also 0.025 ml)

price :

419 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

13B3

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Novus Biologicals product webpage

citations: 89

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig s4i	Gut G, Herrmann M, Pelkmans L. Multiplexed protein maps link subcellular organization to cellular states. Science. 2018;361: pubmed publisher
	Li H, Guglielmetti C, Sei Y, Zilberter M, Le Page L, Shields L, et al. Neurons require glucose uptake and glycolysis in vivo. Cell Rep. 2023;42:112335 pubmed publisher
	Cheng Z, Qin H, Cao W, He H, Zhang S, Yang Y, et al. Intravoxel incoherent motion imaging used to assess tumor microvascular changes after transarterial chemoembolization in a rabbit VX2 liver tumor model. Front Oncol. 2023;13:1114406 pubmed publisher
	Li Y, Chen X, Huang H, Liao L, Chong H, Li G, et al. A feedback loop between NONHSAT024276 and PTBP1 inhibits tumor progression and glycolysis in HCC by increasing the PKM1/PKM2 ratio. Cancer Sci. 2023;114:1519-1540 pubmed publisher
	Sivalingam V, Latif A, Kitson S, McVey R, Finegan K, Marshall K, et al. Hypoxia and hyperglycaemia determine why some endometrial tumours fail to respond to metformin. Br J Cancer. 2020;122:62-71 pubmed publisher
	Zhang H, Wang D, Li M, Plecitá Hlavatá L, D Alessandro A, Tauber J, et al. Metabolic and Proliferative State of Vascular Adventitial Fibroblasts in Pulmonary Hypertension Is Regulated Through a MicroRNA-124/PTBP1 (Polypyrimidine Tract Binding Protein 1)/Pyruvate Kinase Muscle Axis. Circulation. 2017;136:2468-2485 pubmed publisher
	Sung W, Chen P, Liao M, Lee J. Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1. Exp Cell Res. 2017;359:94-100 pubmed publisher
	Minami K, Taniguchi K, Sugito N, Kuranaga Y, Inamoto T, Takahara K, et al. MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget. 2017;8:33064-33077 pubmed publisher
	Takai T, Yoshikawa Y, Inamoto T, Minami K, Taniguchi K, Sugito N, et al. A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells. Int J Mol Sci. 2017;18: pubmed publisher
	Marie Egyptienne D, Chaudary N, KalliomÃ¤ki T, Hedley D, Hill R. Cancer initiating-cells are enriched in the CA9 positive fraction of primary cervix cancer xenografts. Oncotarget. 2017;8:1392-1404 pubmed publisher
	Sugiyama T, Taniguchi K, Matsuhashi N, Tajirika T, Futamura M, Takai T, et al. MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1. Cancer Sci. 2016;107:1767-1775 pubmed publisher
	Semba H, Takeda N, Isagawa T, Sugiura Y, Honda K, Wake M, et al. HIF-1?-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016;7:11635 pubmed publisher
	Christensen D, Calder P, Houghton F. GLUT3 and PKM2 regulate OCT4 expression and support the hypoxic culture of human embryonic stem cells. Sci Rep. 2015;5:17500 pubmed publisher
	Taniguchi K, Sugito N, Kumazaki M, Shinohara H, Yamada N, Matsuhashi N, et al. Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells. Biochim Biophys Acta. 2015;1852:1971-80 pubmed publisher
	Taniguchi K, Ito Y, Sugito N, Kumazaki M, Shinohara H, Yamada N, et al. Organ-specific PTB1-associated microRNAs determine expression of pyruvate kinase isoforms. Sci Rep. 2015;5:8647 pubmed publisher
	Shinohara H, Taniguchi K, Kumazaki M, Yamada N, Ito Y, Otsuki Y, et al. Anti-cancer fatty-acid derivative induces autophagic cell death through modulation of PKM isoform expression profile mediated by bcr-abl in chronic myeloid leukemia. Cancer Lett. 2015;360:28-38 pubmed publisher
	Bruns D, Brown R, Stenmark K, Buttrick P, Walker L. Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction. Am J Physiol Lung Cell Mol Physiol. 2015;308:L158-67 pubmed publisher
	Samba Louaka A, Pereira J, Nahori M, Villiers V, Deriano L, Hamon M, et al. Listeria monocytogenes dampens the DNA damage response. PLoS Pathog. 2014;10:e1004470 pubmed publisher
	Khongkow P, Karunarathna U, Khongkow M, Gong C, Gomes A, Yague E, et al. FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance. Oncogene. 2014;33:4144-55 pubmed publisher
	Masuda Y, Noguchi K, Segawa H, Tanaka N, Katayama K, Mitsuhashi J, et al. Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin. Biochem Biophys Res Commun. 2011;415:305-12 pubmed publisher
	Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res. 2011;39:9605-19 pubmed publisher
	Soares D, Battistella A, Rocca C, Matuo R, Henriques J, Larsen A, et al. Ataxia telangiectasia mutated- and Rad3-related kinase drives both the early and the late DNA-damage response to the monofunctional antitumour alkylator S23906. Biochem J. 2011;437:63-73 pubmed publisher
	Baydoun H, Pancewicz J, Nicot C. Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair. Blood. 2011;117:5897-906 pubmed publisher
	Stoepker C, Hain K, Schuster B, Hilhorst Hofstee Y, Rooimans M, Steltenpool J, et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet. 2011;43:138-41 pubmed publisher
	Anderson V, Walton M, Eve P, Boxall K, Antoni L, Caldwell J, et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res. 2011;71:463-72 pubmed publisher
	Matsumoto Y, Miyamoto T, Sakamoto H, Izumi H, Nakazawa Y, Ogi T, et al. Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly. DNA Repair (Amst). 2011;10:314-21 pubmed publisher
	Luo Y, Chen A, Qiu J. Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death. J Virol. 2011;85:133-45 pubmed publisher
	Kuroda S, Fujiwara T, Shirakawa Y, Yamasaki Y, Yano S, Uno F, et al. Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery. Cancer Res. 2010;70:9339-48 pubmed publisher
	Machida K, McNamara G, Cheng K, Huang J, Wang C, Comai L, et al. Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes. J Immunol. 2010;185:6985-98 pubmed publisher
	Balasubramanian N, Bai P, Buchek G, Korza G, Weller S. Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex. J Virol. 2010;84:12504-14 pubmed publisher
	Gomez Godinez V, Wu T, Sherman A, Lee C, Liaw L, Zhongsheng Y, et al. Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation. Nucleic Acids Res. 2010;38:e202 pubmed publisher
	Palagyi A, Neveling K, Plinninger U, Ziesch A, Targosz B, Denk G, et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer. 2010;9:127 pubmed publisher
	Cha H, Lowe J, Li H, Lee J, Belova G, Bulavin D, et al. Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer Res. 2010;70:4112-22 pubmed publisher
	Lee J, Goodarzi A, Jeggo P, Paull T. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J. 2010;29:574-85 pubmed publisher
	Collaco R, Bevington J, Bhrigu V, Kalman Maltese V, Trempe J. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology. 2009;392:24-33 pubmed publisher
	Waltes R, Kalb R, Gatei M, Kijas A, Stumm M, Sobeck A, et al. Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder. Am J Hum Genet. 2009;84:605-16 pubmed publisher
	Bartkova J, Tommiska J, Oplustilova L, Aaltonen K, Tamminen A, Heikkinen T, et al. Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene. Mol Oncol. 2008;2:296-316 pubmed publisher
	Schwartz R, Carson C, Schuberth C, Weitzman M. Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase. J Virol. 2009;83:6269-78 pubmed publisher
	Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori A, Gerrits B, et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res. 2009;37:2645-57 pubmed publisher
	Karen K, Hoey P, Young C, Hearing P. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol. 2009;83:4565-73 pubmed publisher
	Carson C, Orazio N, Lee D, Suh J, Bekker Jensen S, Araujo F, et al. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection. EMBO J. 2009;28:652-62 pubmed publisher
	Lakdawala S, Schwartz R, Ferenchak K, Carson C, McSharry B, Wilkinson G, et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol. 2008;82:8362-72 pubmed publisher
	Cervelli T, Palacios J, Zentilin L, Mano M, Schwartz R, Weitzman M, et al. Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins. J Cell Sci. 2008;121:349-57 pubmed publisher
	Gregory D, Bachenheimer S. Characterization of mre11 loss following HSV-1 infection. Virology. 2008;373:124-36 pubmed publisher
	Schwartz R, Palacios J, Cassell G, Adam S, Giacca M, Weitzman M. The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. J Virol. 2007;81:12936-45 pubmed
	Gorodetsky E, Calkins S, Ahn J, Brooks P. ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain. DNA Repair (Amst). 2007;6:1698-707 pubmed
	Zhong Z, Jiang W, Cesare A, Neumann A, Wadhwa R, Reddel R. Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem. 2007;282:29314-22 pubmed
	Bentle M, Reinicke K, Dong Y, Bey E, Boothman D. Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone. Cancer Res. 2007;67:6936-45 pubmed
	Cho Y, Hong T, Hong S, Guo H, Yu H, Kim D, et al. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem. 2007;282:20395-406 pubmed
	Bae N, Baumann P. A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends. Mol Cell. 2007;26:323-34 pubmed
	Lin X, Liu C, Gao Q, Zhang X, Wu G, Lee W. RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival. Mol Cell Biol. 2007;27:4905-16 pubmed
	You K, Li L, Kim N, Kang H, Koh K, Chwae Y, et al. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol. 2007;5:e109 pubmed
	Dote H, Burgan W, Camphausen K, Tofilon P. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res. 2006;66:9211-20 pubmed
	Takemura H, Rao V, Sordet O, Furuta T, Miao Z, Meng L, et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006;281:30814-23 pubmed
	Kong L, Meloni A, Nevins J. The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Mol Cell. 2006;22:63-71 pubmed
	Myers J, Cortez D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem. 2006;281:9346-50 pubmed
	Tommiska J, Seal S, Renwick A, Barfoot R, Baskcomb L, Jayatilake H, et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer. 2006;118:2911-6 pubmed
	Al Rashid S, Dellaire G, Cuddihy A, Jalali F, Vaid M, Coackley C, et al. Evidence for the direct binding of phosphorylated p53 to sites of DNA breaks in vivo. Cancer Res. 2005;65:10810-21 pubmed
	Mongiat Artus P, Miquel C, van der Aa M, Buhard O, Hamelin R, Soliman H, et al. Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract. Oncogene. 2006;25:2113-8 pubmed
	Liu Y, Shevchenko A, Shevchenko A, Berk A. Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex. J Virol. 2005;79:14004-16 pubmed
	Kim J, Krasieva T, Kurumizaka H, Chen D, Taylor A, Yokomori K. Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells. J Cell Biol. 2005;170:341-7 pubmed
	Koh K, Kang H, Li L, Kim N, You K, Yang E, et al. Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. Lab Invest. 2005;85:1130-8 pubmed
	van Veelen L, Cervelli T, van de Rakt M, Theil A, Essers J, Kanaar R. Analysis of ionizing radiation-induced foci of DNA damage repair proteins. Mutat Res. 2005;574:22-33 pubmed
	Lilley C, Carson C, Muotri A, Gage F, Weitzman M. DNA repair proteins affect the lifecycle of herpes simplex virus 1. Proc Natl Acad Sci U S A. 2005;102:5844-9 pubmed
	Fasching C, Bower K, Reddel R. Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Res. 2005;65:2722-9 pubmed
	Angele S, Jones C, Reis Filho J, Fulford L, Treilleux I, Lakhani S, et al. Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast. J Clin Pathol. 2004;57:1179-84 pubmed
	Wu X, Avni D, Chiba T, Yan F, Zhao Q, Lin Y, et al. SV40 T antigen interacts with Nbs1 to disrupt DNA replication control. Genes Dev. 2004;18:1305-16 pubmed
	O Connor M, Safari A, Liu D, Qin J, Songyang Z. The human Rap1 protein complex and modulation of telomere length. J Biol Chem. 2004;279:28585-91 pubmed
	Wilkinson D, Weller S. Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage respon. J Virol. 2004;78:4783-96 pubmed
	Dork T, Bendix Waltes R, Wegner R, Stumm M. Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations. Am J Med Genet A. 2004;126A:272-7 pubmed
	Giannini G, Rinaldi C, Ristori E, Ambrosini M, Cerignoli F, Viel A, et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene. 2004;23:2640-7 pubmed
	Carson C, Schwartz R, Stracker T, Lilley C, Lee D, Weitzman M. The Mre11 complex is required for ATM activation and the G2/M checkpoint. EMBO J. 2003;22:6610-20 pubmed
	Uziel T, Lerenthal Y, Moyal L, Andegeko Y, Mittelman L, Shiloh Y. Requirement of the MRN complex for ATM activation by DNA damage. EMBO J. 2003;22:5612-21 pubmed
	Xu X, Stern D. NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors. FASEB J. 2003;17:1842-8 pubmed
	Angele S, Treilleux I, Bremond A, Taniere P, Hall J. Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas. Histopathology. 2003;43:347-53 pubmed
	Evans J, Hearing P. Distinct roles of the Adenovirus E4 ORF3 protein in viral DNA replication and inhibition of genome concatenation. J Virol. 2003;77:5295-304 pubmed
	Stewart G, Wang B, Bignell C, Taylor A, Elledge S. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature. 2003;421:961-6 pubmed
	Anderson L, Perkins N. Regulation of RelA (p65) function by the large subunit of replication factor C. Mol Cell Biol. 2003;23:721-32 pubmed
	Hassane D, Lee R, Pickett C. Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells. Infect Immun. 2003;71:541-5 pubmed
	Richie C, Peterson C, Lu T, Hittelman W, Carpenter P, Legerski R. hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage. Mol Cell Biol. 2002;22:8635-47 pubmed
	Pichierri P, Averbeck D, Rosselli F. DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein. Hum Mol Genet. 2002;11:2531-46 pubmed
	Kim J, Krasieva T, LaMorte V, Taylor A, Yokomori K. Specific recruitment of human cohesin to laser-induced DNA damage. J Biol Chem. 2002;277:45149-53 pubmed
	Stracker T, Carson C, Weitzman M. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature. 2002;418:348-52 pubmed
	Franchitto A, Pichierri P. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol. 2002;157:19-30 pubmed
	Carbone R, Pearson M, Minucci S, Pelicci P. PML NBs associate with the hMre11 complex and p53 at sites of irradiation induced DNA damage. Oncogene. 2002;21:1633-40 pubmed
	Harfst E, Cooper S, Neubauer S, Distel L, Grawunder U. Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome. Mol Immunol. 2000;37:915-29 pubmed
	Tauchi H, Kobayashi J, Morishima K, Matsuura S, Nakamura A, Shiraishi T, et al. The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity. J Biol Chem. 2001;276:12-5 pubmed
	Paull T, Rogakou E, Yamazaki V, Kirchgessner C, Gellert M, Bonner W. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol. 2000;10:886-95 pubmed
	Wang Y, Cortez D, Yazdi P, Neff N, Elledge S, Qin J. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000;14:927-39 pubmed

product information

master code :

NBP1-48308

SKU :

NBP1-48308

product name :

PKM2 Antibody - BSA Free

unit size :

0.1 ml (also 0.025 ml)

description :

The PKM2 Antibody - BSA Free from Novus is a rabbit polyclonal antibody to PKM2. This antibody reacts with bovine,human,mouse,rat. The PKM2 Antibody - BSA Free has been validated for the following applications: Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry,Immunoblotting,Simple Western,Knockdown Validated.

target :

PKM2

category :

Primary Antibodies

buffer :

PBS and 30% Glycerol

clonality :

Polyclonal

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Rabbit

immunogen :

A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450). [Swiss-Prot: P14618]

isotype :

IgG

purity :

Immunogen affinity purified

species :

Bovine,Human,Mouse,Rat

theoretical molecular weight :

60 kDa

gene symbol :

PKM

accessionNumbers :

P14618, P52480

applications :

Western Blot,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Flow Cytometry,Immunohistochemistry,Immunoblotting,Simple Western,Knockdown Validated

USD :

419 USD

alt names :

CTHBP, Cytosolic thyroid hormone-binding protein, MGC3932, OIP-3, OIP3EC 2.7.1.40, Opa-interacting protein 3, PK, muscle type, PK2, PK3PKM, Pyruvate kinase 2/3, pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, pyruvate kinase, muscle, TCB, THBP1p58, Thyroid hormone-binding protein 1, thyroid hormone-binding protein, cytosolic, Tumor M2-PK

storage :

Store at -20C. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage