product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Mouse Monoclonal PHD2/HIF Prolyl Hydroxylase 2 Antibody
catalog :
NBP1-30328C
quantity :
0.1 ml
price :
379.00 USD
clonality :
monoclonal
host :
mouse
reactivity :
human, mouse
application :
western blot, immunohistochemistry
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NBP1-30328C
Product Name :
Mouse Monoclonal PHD2/HIF Prolyl Hydroxylase 2 Antibody
List Price :
379.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
C1orf12HPH2, EC 1.14.11.-, EC 1.14.11, egl nine homolog 1, egl nine homolog 1 (C. elegans), egl nine-like protein 1, ECYT3, HIF-prolyl hydroxylase 2, HIF-PH2, HIFPH2 EGL nine (C.elegans) homolog 1, HIF prolyl hydroxylase 2, HPH-2, Hypoxia-inducible factor prolyl hydroxylase 2, PHD2SM20, Prolyl hydroxylase domain-containing protein 2, SM-20, zinc finger MYND domain-containing protein 6, ZMYND6DKFZp761F179
Background :
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
366G/76/3
Concentration :
1.0 mg/ml
Conjugate :
DyLight 650
Application Summary :
Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin
Host :
Mouse
Immunogen :
A peptide within residues 1-50 of human PHD2/HIF Prolyl Hydroxylase 2. [Swiss-Prot# Q9GZT9]
Isotype :
IgG1
Preservative :
No Preservative
Purity :
Protein G purified
CrossReactivity :
Human. Immunogen sequence has 83% identity with mouse.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q9GZT9
TMW :
46 kDa
Uses :
This HIF Prolyl Hydroxylase 2 antibody is useful for Immunohistochemistry paraffin embedded sections, and Western Blot analysis where a band can be seen at ~46 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Research Area :
Cancer, Chromatin Research, Hypoxia
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.