product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Histone H3 [Trimethyl Lys9] Antibody (6F12-H4)
catalog :
NBP1-30141
quantity :
0.1 ml (also 0.025 ml)
price :
429 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6F12-H4
reactivity :
human, mouse, rat, pigs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; pigs ; 1:100; fig 3c
  • western blot; pigs ; fig 1b
No J, Choi M, Kwon D, Yoo J, Yang B, Park J, et al. Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming. J Reprod Dev. 2015;61:90-8 pubmed publisher
  • western blot; mouse
Krapivinsky G, Krapivinsky L, Manasian Y, Clapham D. The TRPM7 chanzyme is cleaved to release a chromatin-modifying kinase. Cell. 2014;157:1061-72 pubmed publisher
  • western blot; mouse
Cornacchia D, Dileep V, Quivy J, Foti R, Tili F, Santarella Mellwig R, et al. Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells. EMBO J. 2012;31:3678-90 pubmed publisher
Marsoner T, Yedavalli P, Masnovo C, Fink S, Schmitzer K, Campbell C. Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast. Mol Biol Cell. 2022;33:ar85 pubmed publisher
Hollenstein D, Veis J, Romanov N, G xe9 recov xe1 G, Ogris E, Hartl M, et al. PP2ARts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast. Microbiol Res. 2022;260:127031 pubmed publisher
Saleh T, El Sadoni M, Alhesa A, Awad H, Jaradat M, Al Hazaimeh M, et al. Expression of Senescence and Apoptosis Biomarkers in Synchronous Bilateral Breast Cancer: A Case Report. Curr Oncol. 2021;28:3836-3845 pubmed publisher
Hollenstein D, Gérecová G, Romanov N, Ferrari J, Veis J, Janschitz M, et al. A phosphatase-centric mechanism drives stress signaling response. EMBO Rep. 2021;22:e52476 pubmed publisher
Božič J, Motaln H, Janež A, Markič L, Tripathi P, Yamoah A, et al. Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. Brain. 2021;: pubmed publisher
Saleh T, Alhesa A, Al Balas M, Abuelaish O, Mansour A, Awad H, et al. Expression of therapy-induced senescence markers in breast cancer samples upon incomplete response to neoadjuvant chemotherapy. Biosci Rep. 2021;41: pubmed publisher
Perea García A, Miró P, Jiménez Lorenzo R, Martínez Pastor M, Puig S. Sequential recruitment of the mRNA decay machinery to the iron-regulated protein Cth2 in Saccharomyces cerevisiae. Biochim Biophys Acta Gene Regul Mech. 2020;1863:194595 pubmed publisher
Qian Y, Li Y, Zheng C, Lu T, Sun R, Mao Y, et al. High methylation levels of histone H3 lysine 9 associated with activation of hypoxia-inducible factor 1α (HIF-1α) predict patients' worse prognosis in human hepatocellular carcinomas. Cancer Genet. 2020;245:17-26 pubmed publisher
Bergstrand S, Böhm S, Malmgren H, Norberg A, Sundin M, Nordgren A, et al. Biallelic mutations in WRAP53 result in dysfunctional telomeres, Cajal bodies and DNA repair, thereby causing Hoyeraal-Hreidarsson syndrome. Cell Death Dis. 2020;11:238 pubmed publisher
O Dea R, Santocanale C. Non-canonical regulation of homologous recombination DNA repair by the USP9X deubiquitylase. J Cell Sci. 2020;133: pubmed publisher
Cebrià Costa J, Pascual Reguant L, Gonzalez Perez A, Serra Bardenys G, Querol J, Cosín M, et al. LOXL2-mediated H3K4 oxidation reduces chromatin accessibility in triple-negative breast cancer cells. Oncogene. 2020;39:79-121 pubmed publisher
Jin S, Cordes N. ATM controls DNA repair and mitochondria transfer between neighboring cells. Cell Commun Signal. 2019;17:144 pubmed publisher
Hollenstein D, Gómez Sánchez R, Ciftci A, Kriegenburg F, Mari M, Torggler R, et al. Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae. J Cell Sci. 2019;132: pubmed publisher
Zhang W, Liao C, Chtatou H, Incrocci L, van Gent D, Van Weerden W, et al. Apalutamide Sensitizes Prostate Cancer to Ionizing Radiation via Inhibition of Non-Homologous End-Joining DNA Repair. Cancers (Basel). 2019;11: pubmed publisher
van Drogen F, Mishra R, Rudolf F, Walczak M, Lee S, Reiter W, et al. Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5. J Cell Biol. 2019;218:3117-3133 pubmed publisher
Janschitz M, Romanov N, Varnavides G, Hollenstein D, Gérecová G, Ammerer G, et al. Novel interconnections of HOG signaling revealed by combined use of two proteomic software packages. Cell Commun Signal. 2019;17:66 pubmed publisher
He B, Gnawali N, Hinman A, Mattingly A, Osimani A, Cimini D. Chromosomes missegregated into micronuclei contribute to chromosomal instability by missegregating at the next division. Oncotarget. 2019;10:2660-2674 pubmed publisher
Yost K, Clatterbuck Soper S, Walker R, Pineda M, Zhu Y, Ester C, et al. Rapid and reversible suppression of ALT by DAXX in osteosarcoma cells. Sci Rep. 2019;9:4544 pubmed publisher
Zhou M, Li Y, Lin S, Chen Y, Qian Y, Zhao Z, et al. H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci. 2019;: pubmed publisher
Zhao C, Shi J, Zhou R, He X, Yang H, Wu Z. DZNep and UNC0642 enhance in vitro developmental competence of cloned pig embryos. Reproduction. 2018;157:359-369 pubmed publisher
Stixova L, Komůrková D, Svobodová Kovaříková A, Bartova E. UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation. Chromosome Res. 2019;27:41-55 pubmed publisher
Shine E, Xue M, Patel J, Healy A, Surovtseva Y, Herzon S, et al. Model Colibactins Exhibit Human Cell Genotoxicity in the Absence of Host Bacteria. ACS Chem Biol. 2018;13:3286-3293 pubmed publisher
Li Y, Guo D, Sun R, Chen P, Qian Q, Fan H. Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci. 2019;64:439-446 pubmed publisher
Chakraborty P, Huang J, Hiom K. DHX9 helicase promotes R-loop formation in cells with impaired RNA splicing. Nat Commun. 2018;9:4346 pubmed publisher
Peterson J, Wang D, Shettigar V, Roof S, Canan B, Bakkar N, et al. NF-κB inhibition rescues cardiac function by remodeling calcium genes in a Duchenne muscular dystrophy model. Nat Commun. 2018;9:3431 pubmed publisher
Bayley R, Blakemore D, Cancian L, Dumon S, Volpe G, Ward C, et al. MYBL2 Supports DNA Double Strand Break Repair in Hematopoietic Stem Cells. Cancer Res. 2018;78:5767-5779 pubmed publisher
Kang Y, Yan C. Regulation of DNA repair in the absence of classical non-homologous end joining. DNA Repair (Amst). 2018;68:34-40 pubmed publisher
Kai Y, Li Y, Sun T, Yin W, Mao Y, Li J, et al. A medial prefrontal cortex-nucleus acumens corticotropin-releasing factor circuitry for neuropathic pain-increased susceptibility to opioid reward. Transl Psychiatry. 2018;8:100 pubmed publisher
Bueno M, Baldascini M, Richard S, Lowndes N. Recruitment of lysine demethylase 2A to DNA double strand breaks and its interaction with 53BP1 ensures genome stability. Oncotarget. 2018;9:15915-15930 pubmed publisher
Borude P, Bhushan B, Apte U. DNA Damage Response Regulates Initiation of Liver Regeneration Following Acetaminophen Overdose. Gene Expr. 2018;18:115-123 pubmed publisher
Biswas U, Stevense M, Jessberger R. SMC1α Substitutes for Many Meiotic Functions of SMC1β but Cannot Protect Telomeres from Damage. Curr Biol. 2018;28:249-261.e4 pubmed publisher
Hassan S, Esch A, Liby T, Gray J, Heiser L. Pathway-Enriched Gene Signature Associated with 53BP1 Response to PARP Inhibition in Triple-Negative Breast Cancer. Mol Cancer Ther. 2017;16:2892-2901 pubmed publisher
Bao X, Siprashvili Z, Zarnegar B, Shenoy R, Rios E, Nady N, et al. CSNK1a1 Regulates PRMT1 to Maintain the Progenitor State in Self-Renewing Somatic Tissue. Dev Cell. 2017;43:227-239.e5 pubmed publisher
Sivanand S, Rhoades S, Jiang Q, Lee J, Benci J, Zhang J, et al. Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination. Mol Cell. 2017;67:252-265.e6 pubmed publisher
Foskolou I, Jorgensen C, Leszczynska K, Olcina M, Tarhonskaya H, Haisma B, et al. Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication. Mol Cell. 2017;66:206-220.e9 pubmed publisher
Romanov N, Hollenstein D, Janschitz M, Ammerer G, Anrather D, Reiter W. Identifying protein kinase-specific effectors of the osmostress response in yeast. Sci Signal. 2017;10: pubmed publisher
Ho T, Warr M, Adelman E, Lansinger O, Flach J, Verovskaya E, et al. Autophagy maintains the metabolism and function of young and old stem cells. Nature. 2017;543:205-210 pubmed publisher
Li S, Shu F, Li Z, Jaafar L, Zhao S, Dynan W. Cell-type specific role of the RNA-binding protein, NONO, in the DNA double-strand break response in the mouse testes. DNA Repair (Amst). 2017;51:70-78 pubmed publisher
Cheng L, Tan V, Ganesan S, Drake J. Integrating phosphoproteomics into the clinical management of prostate cancer. Clin Transl Med. 2017;6:9 pubmed publisher
Kang Y, Balter B, Csizmadia E, Haas B, Sharma H, Bronson R, et al. Contribution of classical end-joining to PTEN inactivation in p53-mediated glioblastoma formation and drug-resistant survival. Nat Commun. 2017;8:14013 pubmed publisher
Demaria M, O Leary M, Chang J, Shao L, Liu S, Alimirah F, et al. Cellular Senescence Promotes Adverse Effects of Chemotherapy and Cancer Relapse. Cancer Discov. 2017;7:165-176 pubmed publisher
Jaafar L, Li Z, Li S, Dynan W. SFPQ•NONO and XLF function separately and together to promote DNA double-strand break repair via canonical nonhomologous end joining. Nucleic Acids Res. 2017;45:1848-1859 pubmed publisher
Dilley R, Verma P, Cho N, Winters H, Wondisford A, Greenberg R. Break-induced telomere synthesis underlies alternative telomere maintenance. Nature. 2016;539:54-58 pubmed publisher
Moureau S, Luessing J, Harte E, Voisin M, Lowndes N. A role for the p53 tumour suppressor in regulating the balance between homologous recombination and non-homologous end joining. Open Biol. 2016;6: pubmed
Moreno A, Carrington J, Albergante L, Al Mamun M, Haagensen E, Komseli E, et al. Unreplicated DNA remaining from unperturbed S phases passes through mitosis for resolution in daughter cells. Proc Natl Acad Sci U S A. 2016;113:E5757-64 pubmed publisher
Nandakumar V, Hansen N, Glenn H, Han J, Helland S, Hernandez K, et al. Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep. 2016;6:30593 pubmed publisher
Saredi G, Huang H, Hammond C, Alabert C, Bekker Jensen S, Forne I, et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature. 2016;534:714-718 pubmed publisher
Pederiva C, Böhm S, Julner A, Farnebo M. Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ. 2016;23:1648-57 pubmed publisher
Rassoolzadeh H, Böhm S, Hedström E, Gad H, Helleday T, Henriksson S, et al. Overexpression of the scaffold WD40 protein WRAP53β enhances the repair of and cell survival from DNA double-strand breaks. Cell Death Dis. 2016;7:e2267 pubmed publisher
Avila A, Illing A, Becker F, Maerz L, Morita Y, Philipp M, et al. Xpg limits the expansion of haematopoietic stem and progenitor cells after ionising radiation. Nucleic Acids Res. 2016;44:6252-61 pubmed publisher
Byrd P, Stewart G, Smith A, Eaton C, Taylor A, Guy C, et al. A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development. PLoS Genet. 2016;12:e1005945 pubmed publisher
Surovtseva Y, Jairam V, Salem A, Sundaram R, Bindra R, Herzon S. Characterization of Cardiac Glycoside Natural Products as Potent Inhibitors of DNA Double-Strand Break Repair by a Whole-Cell Double Immunofluorescence Assay. J Am Chem Soc. 2016;138:3844-55 pubmed publisher
Mackenzie K, Carroll P, Lettice L, TarnauskaitÄ— Å, Reddy K, Dix F, et al. Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response. EMBO J. 2016;35:831-44 pubmed publisher
Rassoolzadeh H, Coucoravas C, Farnebo M. The proximity ligation assay reveals that at DNA double-strand breaks WRAP53β associates with γH2AX and controls interactions between RNF8 and MDC1. Nucleus. 2015;6:417-24 pubmed publisher
Park J, Welner R, Chan M, Troppito L, Staber P, Tenen D, et al. The DNA Ligase IV Syndrome R278H Mutation Impairs B Lymphopoiesis via Error-Prone Nonhomologous End-Joining. J Immunol. 2016;196:244-55 pubmed publisher
Hausmann C, Temme A, Cordes N, Eke I. ILKAP, ILK and PINCH1 control cell survival of p53-wildtype glioblastoma cells after irradiation. Oncotarget. 2015;6:34592-605 pubmed publisher
Hedstrom E, Pederiva C, Farnebo J, Nodin B, Jirstrom K, Brennan D, et al. Downregulation of the cancer susceptibility protein WRAP53β in epithelial ovarian cancer leads to defective DNA repair and poor clinical outcome. Cell Death Dis. 2015;6:e1892 pubmed publisher
Boysen G, Barbieri C, Prandi D, Blattner M, Chae S, Dahija A, et al. SPOP mutation leads to genomic instability in prostate cancer. elife. 2015;4: pubmed publisher
Anbalagan S, Biasoli D, Leszczynska K, Mukherjee S, Hammond E. In Vitro Radiosensitization of Esophageal Cancer Cells with the Aminopeptidase Inhibitor CHR-2797. Radiat Res. 2015;184:259-65 pubmed publisher
Hartlerode A, Morgan M, Wu Y, Buis J, Ferguson D. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat Struct Mol Biol. 2015;22:736-43 pubmed publisher
Dickreuter E, Eke I, Krause M, Borgmann K, van Vugt M, Cordes N. Targeting of β1 integrins impairs DNA repair for radiosensitization of head and neck cancer cells. Oncogene. 2016;35:1353-62 pubmed publisher
Wilson C, Jurk D, Fullard N, Banks P, Page A, Luli S, et al. NFκB1 is a suppressor of neutrophil-driven hepatocellular carcinoma. Nat Commun. 2015;6:6818 pubmed publisher
Raymond A, Benhamouche S, Neaud V, Di Martino J, Javary J, Rosenbaum J. Reptin regulates DNA double strand breaks repair in human hepatocellular carcinoma. PLoS ONE. 2015;10:e0123333 pubmed publisher
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Luo Z, Feng X, Wang H, Xu W, Zhao Y, Ma W, et al. Mir-23a induces telomere dysfunction and cellular senescence by inhibiting TRF2 expression. Aging Cell. 2015;14:391-9 pubmed publisher
Kondratova A, Watanabe T, Marotta M, Cannon M, Segall A, Serre D, et al. Replication fork integrity and intra-S phase checkpoint suppress gene amplification. Nucleic Acids Res. 2015;43:2678-90 pubmed publisher
Marin Vicente C, Domingo Prim J, Eberle A, Visa N. RRP6/EXOSC10 is required for the repair of DNA double-strand breaks by homologous recombination. J Cell Sci. 2015;128:1097-107 pubmed publisher
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Herraiz A, Belles X, Piulachs M. Chorion formation in panoistic ovaries requires windei and trimethylation of histone 3 lysine 9. Exp Cell Res. 2014;320:46-53 pubmed publisher
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product information
brand :
Novus
master code :
NBP1-30141
SKU :
NBP1-30141
product name :
Histone H3 [Trimethyl Lys9] Antibody (6F12-H4)
description :
The Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) from Novus Biologicals is a mouse monoclonal antibody to Histone H3. This antibody reacts with human, mouse, rat, porcine, c. elegans, drosophila, invertebrate, mammal, yeast. The Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.
target :
Histone H3
category :
Primary Antibodies
sizes available :
0.1 ml (also 0.025 ml)
buffer :
Tris-Glycine and 0.15M NaCl
clonality :
Monoclonal
clone :
6F12-H4
concentration :
1 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
This Histone H3 [Trimethyl Lys9] antibody (6F12-H4) was raised against a synthetic peptide made to an N-terminal region of Histone H3 (between amino acids 1-50). [UniProt# P84243]
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Human, Mouse, Rat, Porcine, C. elegans, Drosophila, Invertebrate, Mammal, Yeast
theoretical molecular weight :
15 kDa
gene symbol :
H3C14
accessionNumbers :
P84243, P84244
applications :
Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin
USD :
410
USD 2023 :
429 USD
alt names :
H3, H3 histone, family 3A, H3.3A, H3.3B, H3F2, H3F3, H3F3B, H3FM, H3K9Me3, HIST2H3C, histone H3.3, MGC87782, MGC87783
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.