product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal PDK4 Antibody
catalog :
NBP1-07047F
quantity :
0.1 ml
price :
299.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
FITC
reactivity :
human, mouse, rat, cow
application :
western blot, immunocytochemistry
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NBP1-07047F
Product Name :
Rabbit Polyclonal PDK4 Antibody
List Price :
299.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
EC 2.7.11, EC 2.7.11.2, FLJ40832, Pyruvate dehydrogenase kinase isoform 4, pyruvate dehydrogenase kinase, isoenzyme 4, pyruvate dehydrogenase kinase, isozyme 4, pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
Background :
PDK4 (pyruvate dehydrogenase kinase isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptional initiation complex responsible for PDK4 activation. Insulin promotes inactivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Conjugate :
FITC
Application Summary :
Western Blot, ICC/IF (Negative)
Host :
Rabbit
Immunogen :
Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 100-150). [Swiss-Prot# Q16654]
Localization :
Mitochondrion matrix.
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human and Mouse. Predicted to react with bovine based on 100% sequence homology. Immunogen sequence has 94% homology to rat.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q16654
TMW :
49 kDa
Uses :
This PDK4 antibody is useful for Western blot, where a band is seen at ~49 kDa. This product is not recommended for ICC, as nonspecific nuclear staining was observed in HeLa cells.
Research Area :
Cancer, Lipid and Metabolism, Mitochondrial Markers, Protein Kinase, Signal Transduction
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.