This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Methionine Sulfoxide Antibody
catalog :
NBP1-06707
quantity :
0.1 ml
price :
299 USD
clonality :
polyclonal
host :
rabbit
reactivity :
rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 4
| Published Application/Species/Dilution | Reference |
|---|---|
Salama S, Snapka R. Amino acid chloramine damage to proliferating cell nuclear antigen in mammalian cells. In Vivo. 2012;26:501-17 pubmed
| |
product information
Catalog Number :
NBP1-06707
Product Name :
Methionine Sulfoxide Antibody
List Price :
299 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
MetO, MetO, MetO, MetO, MetO, MetO
Background :
Methionine (Met) oxidation is one of the most common posttranslational modifications to proteins that may alter protein structure and function. Accumulation of proteins containing methionine sulfoxide (MetO) is known to occur during oxidative stress. If the reversal of protein MetO to Met is not sufficient it may cause proteins to loose function, aggregate and be toxic to the cell. Methionine oxidation in proteins has been associated with neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and Creutzfeldt-Jakob disease.
Clonality :
Polyclonal
Applications :
Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Host :
Rabbit
Immunogen :
Oxidized methionine rich zein protein from Zea mays
Preservative :
0.05% Sodium Azide
Purity :
Whole antisera
CrossReactivity :
Human, mouse, rat and yeast.
Specificity :
Specific for all methionine sulfoxide containing proteins and peptides.
Storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Uses :
This antibody is useful for Western blot. The size of the band is dependent on the size of the protein that has undergone methionine oxidation. It has also been used for immunohistochemistry on paraffin sections (rat brain with heat mediated antigen retrieval). Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Research Area :
Alzheimers Research, Amino Acids, Drugs and other small molecules, Lipid and Metabolism, Neurodegeneration, Neuroscience, Signal Transduction
References Product :
Doronina VA, Staniforth GL. Oxidative stress conditions increase the frequency of de novo formation of the yeast [PSI+] prion Molecular Microbiology. 2015 (WB, Yeast) Details: Methionine Sulfoxide antibody used in WB assay for the detection of oxidation of Sup35 translation termination factor in experiments involving the de novo formation of the yeast [PSI+] prion. Fig 1B shows the blot of alpha MetO / Sup35 oxidation in [PIN+ ] [psi+] control strain containing the Sup35NM-GFP plasmid following growth in the presence of 100 uM H2O2 or 100 uM menadione for 16 hours. Antibody also used for detecting the basal levels of MetO in WT and antioxidant mutants namely sod1, sod2, sod1 sod2, ctt1, ctt1 cta1 and tsa1 tsa2 mutants (Fig. 3A), and in methionine sulphoxide reductase/MSRA overexpressing mutants (Fig 3B). Callizot N, Combes M, Steinschneider R, Poindron P. Operational dissection of beta-amyloid cytopathic effects on cultured neurons. J Neurosci Res 2013 May [PMID: 23404368] (ICC/IF) Nakaso K, Tajima N, Ito S et al. Dopamine-Mediated Oxidation of Methionine 127 in alpha-Synuclein Causes Cytotoxicity and Oligomerization of alpha-Synuclein. PLoS One 2013 [PMID: 23457458] (WB, Rat) Salama SA, Snapka RM. Amino Acid chloramine damage to proliferating cell nuclear antigen in Mammalian cells In Vivo 2012 Jul [PMID: 22773562] Sideri TC, Koloteva-Levine N, Tuite MF, Grant CM. Methionine oxidation of Sup35 induces formation of the [PSI+] prion in a yeast peroxiredoxin mutant. J Biol Chem. 2011 Aug 9. [PMID: 21832086]
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.
questions and comments