SREBP1 Antibody (2A4) | Novus Biologicals NB600-582 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

SREBP1 Antibody (2A4)

catalog :

NB600-582

quantity :

0.1 ml (also 0.025 ml)

price :

349 USD

clonality :

monoclonal

host :

mouse

conjugate :

DyLight 550

clone name :

2A4

reactivity :

hamsters, human, mouse, rat, dogs, chicken

application :

western blot, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Novus Biologicals product webpage

citations: 41

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:1000; loading ...; fig 1c	Yang N, Li C, Li H, Liu M, Cai X, Cao F, et al. Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. Front Pharmacol. 2019;10:709 pubmed publisher
western blot; human; loading ...; fig 1g	Cianciola N, Chung S, Manor D, Carlin C. Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum. J Virol. 2017;91: pubmed publisher
western blot; rat	Li Z, Dungan C, Carrier B, Rideout T, Williamson D. Alpha-lipoic acid supplementation reduces mTORC1 signaling in skeletal muscle from high fat fed, obese Zucker rats. Lipids. 2014;49:1193-201 pubmed publisher
western blot; hamsters	Rideout T, Ramprasath V, Griffin J, Browne R, Harding S, Jones P. Phytosterols protect against diet-induced hypertriglyceridemia in Syrian golden hamsters. Lipids Health Dis. 2014;13:5 pubmed publisher
western blot; mouse	Wen S, Jadhav K, Williamson D, Rideout T. Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice. J Lipids. 2013;2013:908048 pubmed publisher
western blot; mouse	Pajvani U, Qiang L, Kangsamaksin T, Kitajewski J, Ginsberg H, Accili D. Inhibition of Notch uncouples Akt activation from hepatic lipid accumulation by decreasing mTorc1 stability. Nat Med. 2013;19:1054-60 pubmed publisher
western blot; dogs	Raksaseri P, Chatsudthipong V, Muanprasat C, Soodvilai S. Activation of liver X receptors reduces CFTR-mediated Cl(-) transport in kidney collecting duct cells. Am J Physiol Renal Physiol. 2013;305:F583-91 pubmed publisher
western blot; mouse	Liu Y, Major A, Zienkiewicz J, Gabriel C, Veach R, Moore D, et al. Nuclear transport modulation reduces hypercholesterolemia, atherosclerosis, and fatty liver. J Am Heart Assoc. 2013;2:e000093 pubmed publisher
western blot; mouse	Herman M, Peroni O, Villoria J, SchÃ¶n M, Abumrad N, BlÃ¼her M, et al. A novel ChREBP isoform in adipose tissue regulates systemic glucose metabolism. Nature. 2012;484:333-8 pubmed publisher
western blot; rat	Gosmain Y, Dif N, Berbe V, Loizon E, Rieusset J, Vidal H, et al. Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues. J Lipid Res. 2005;46:697-705 pubmed
western blot; chicken	Zhang Y, Hillgartner F. Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens. J Nutr. 2004;134:2205-10 pubmed
western blot; human	Demoulin J, Ericsson J, Kallin A, Rorsman C, Ronnstrand L, Heldin C. Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins. J Biol Chem. 2004;279:35392-402 pubmed
western blot; chicken	Yin L, Zhang Y, Hillgartner F. Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes. J Biol Chem. 2002;277:19554-65 pubmed
western blot; human	Sato R, Yang J, Wang X, Evans M, Ho Y, Goldstein J, et al. Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1). J Biol Chem. 1994;269:17267-73 pubmed
western blot; hamsters	Sheng Z, Otani H, Brown M, Goldstein J. Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver. Proc Natl Acad Sci U S A. 1995;92:935-8 pubmed
	Bosc C, Broin N, Fanjul M, Saland E, Farge T, Courdy C, et al. Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites. Nat Commun. 2020;11:4056 pubmed publisher
	Srinivasan M, Bhopale K, Caracheo A, Amer S, Khan S, Kaphalia L, et al. Activation of AMP-activated protein kinase attenuates ethanol-induced ER/oxidative stress and lipid phenotype in human pancreatic acinar cells. Biochem Pharmacol. 2020;180:114174 pubmed publisher
	Shen B, Feng H, Cheng J, Li Z, Jin M, Zhao L, et al. Geniposide alleviates non-alcohol fatty liver disease via regulating Nrf2/AMPK/mTOR signalling pathways. J Cell Mol Med. 2020;24:5097-5108 pubmed publisher
	Park Y, An M, Kim J, Lim Y. Antiobesity effect of ethanolic extract of Ramulus mori in differentiated 3T3-L1 adipocytes and high-fat diet-induced obese mice. J Ethnopharmacol. 2020;251:112542 pubmed publisher
	Lin R, Jia Y, Wu F, Meng Y, Sun Q, Jia L. Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats. Int J Environ Res Public Health. 2019;16: pubmed publisher
	Srinivasan M, Bhopale K, Amer S, Wan J, Kaphalia L, Ansari G, et al. Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice. Biomolecules. 2019;9: pubmed publisher
	Gouw A, Margulis K, Liu N, Raman S, Mancuso A, Toal G, et al. The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth. Cell Metab. 2019;30:556-572.e5 pubmed publisher
	Pham D, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells. Cell Death Dis. 2019;10:537 pubmed publisher
	Li Y, Xu Y, Jadhav K, Zhu Y, Yin L, Zhang Y. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis. Arterioscler Thromb Vasc Biol. 2019;39:1574-1587 pubmed publisher
	Chuang W, Liu Y, Huang C, Lo C, Yao H, Chen H, et al. Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet. J Agric Food Chem. 2019;67:7136-7146 pubmed publisher
	Sengupta M, Griffett K, Flaveny C, Burris T. Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease. ACS Pharmacol Transl Sci. 2018;1:50-60 pubmed publisher
	Li C, Yen C, Fan C, Chuang W, Huang C, Chen H, et al. 14-Deoxy-11,12-didehydroandrographolide suppresses adipogenesis of 3 T3-L1 preadipocytes by inhibiting CCAAT/enhancer-binding protein β activation and AMPK-mediated mitotic clonal expansion. Toxicol Appl Pharmacol. 2018;359:82-90 pubmed publisher
	Ikenoue T, Terakado Y, Zhu C, Liu X, Ohsugi T, Matsubara D, et al. Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation. Sci Rep. 2018;8:2021 pubmed publisher
	Khound R, Shen J, Song Y, Santra D, Su Q. Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway. Mol Nutr Food Res. 2017;: pubmed publisher
	Yang Y, Yang Q, Yang J, Ma Y, Ding G. Angiotensin II induces cholesterol accumulation and injury in podocytes. Sci Rep. 2017;7:10672 pubmed publisher
	Ahmad F, Patrick S, Sheikh T, Sharma V, Pathak P, Malgulwar P, et al. Telomerase reverse transcriptase (TERT) - enhancer of zeste homolog 2 (EZH2) network regulates lipid metabolism and DNA damage responses in glioblastoma. J Neurochem. 2017;143:671-683 pubmed publisher
	Janssens S, Ciapaite J, Wolters J, van Riel N, Nicolay K, Prompers J. An In Vivo Magnetic Resonance Spectroscopy Study of the Effects of Caloric and Non-Caloric Sweeteners on Liver Lipid Metabolism in Rats. Nutrients. 2017;9: pubmed publisher
	Dungan C, Li J, Williamson D. Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling. Lipids. 2016;51:905-12 pubmed publisher
	Mahmood S, Birkaya B, Rideout T, Patel M. Lack of mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in the hepatic de novo lipogenic pathway. Am J Physiol Endocrinol Metab. 2016;311:E117-27 pubmed publisher
	Goedeke L, Rotllan N, CanfrÃ¡n Duque A, Aranda J, RamÃrez C, Araldi E, et al. MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nat Med. 2015;21:1280-9 pubmed publisher
	Casali B, Corona A, Mariani M, Karlo J, Ghosal K, Landreth G. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease. J Neurosci. 2015;35:9173-81 pubmed publisher
	Dungan C, Li Z, Wright D, Williamson D. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve. 2016;53:107-17 pubmed publisher
	Karanth S, Tran V, Kuberan B, Schlegel A. Polyunsaturated fatty acyl-coenzyme As are inhibitors of cholesterol biosynthesis in zebrafish and mice. Dis Model Mech. 2013;6:1365-77 pubmed publisher
	Kim H, Miyazaki M, Ntambi J. Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism. J Lipid Res. 2002;43:1750-7 pubmed
	Yabe D, Brown M, Goldstein J. Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins. Proc Natl Acad Sci U S A. 2002;99:12753-8 pubmed publisher
	Shimano H, Shimomura I, Hammer R, Herz J, Goldstein J, Brown M, et al. Elevated levels of SREBP-2 and cholesterol synthesis in livers of mice homozygous for a targeted disruption of the SREBP-1 gene. J Clin Invest. 1997;100:2115-24 pubmed

image

image 1 :

Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY licence.

product information

brand :

Novus

SKU :

NB600-582

product name :

SREBP1 Antibody (2A4)

unit sizes :

0.1 ml (also 0.025 ml)

description :

The SREBP1 Antibody (2A4) from Novus is a mouse monoclonal antibody to SREBP1. This antibody reacts with human, mouse, rat, canine, chicken, hamster, monkey, golden syrian hamster. The SREBP1 Antibody (2A4) has been validated for the following applications: Western Blot, Simple Western, Immunohistochemistry-Frozen, Knockout Validated.

target :

SREBP1

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

2A4

concentration :

1 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]

isotype :

IgG1 Kappa

purity :

Protein G purified

species :

Human, Mouse, Rat, Canine, Chicken, Hamster, Monkey, Golden Syrian Hamster

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

uses :

This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The use of this antibody in IHC paraffin embedded tissue has been questionable.

gene symbol :

SREBF1

catalog number base :

NB600-582

accessionNumbers :

P36956

applications :

Western Blot, Simple Western, Immunohistochemistry-Frozen, Knockout Validated

USD :

349 USD

default size :

TRUE

alt names :

BHLHD1, Class D basic helix-loop-helix protein 1, SREBP 1c, SREBP-1, SREBP1bHLHd1SREBP-1c, sterol regulatory element binding protein-1, sterol regulatory element binding transcription factor 1, sterol regulatory element-binding protein 1, Sterol regulatory element-binding transcription factor 1

more info or order :

Novus Biologicals product webpage