Mouse Monoclonal beta-Actin Antibody (AC-15) [DyLight 550] | Novus Biologicals NB600-501R product information

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product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal beta-Actin Antibody (AC-15) [DyLight 550]

catalog :

NB600-501R

quantity :

0.1 ml

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

DyLight 550

clone name :

AC-15

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 6	Pastore N, Brady O, Diab H, Martina J, Sun L, Huynh T, et al. TFEB and TFE3 cooperate in the regulation of the innate immune response in activated macrophages. Autophagy. 2016;12:1240-58 pubmed publisher
western blot; mouse; fig 1	Lin C, Chen Y, Lin C, Chen Y, Lo G, Lee P, et al. Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy. Sci Rep. 2015;5:15807 pubmed publisher
western blot; human; fig 6	Kao Y, Chang B, Liang J, Tsai H, Lee Y, Lin R, et al. Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid Î²-oxidation. PLoS Pathog. 2015;11:e1004750 pubmed publisher

product information

Catalog Number :

NB600-501R

Product Name :

Mouse Monoclonal beta-Actin Antibody (AC-15) [DyLight 550]

List Price :

369 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ACTB, actin, beta, actin, cytoplasmic 1, Beta Actin, beta cytoskeletal actin, Beta-actin, BRWS1, PS1TP5-binding protein 1, PS1TP5BP1

Buffer :

50mM Sodium Borate

Clonality :

Monoclonal

Clone :

AC-15

Conjugate :

DyLight 550

Application Summary :

ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot

Epitope :

N-terminus of the beta isoform of actin.

Host :

Mouse

Immunogen :

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Isotype :

IgG1

Preservative :

0.05% Sodium Azide

Purity :

Ascites

CrossReactivity :

Bovine, Canine, Chicken, Drosophila (Negative), Feline, Fish, Guinea Pig, Hamster, Human, Leech, Mammal, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Squirrel, Zebrafish

Specificity :

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

Storage :

Store at 4C in the dark.

Swissprot Human :

P60709

TMW :

Uses :

This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. Molecular weight of beta actin is 42kD. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is not suitable for formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives.

Research Area :

Cellular Markers, Cytoskeleton Markers, Loading Controls, Signal Transduction