Mouse Monoclonal beta-Actin Antibody (AC-15) [HRP] | Novus Biologicals NB600-501H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Mouse Monoclonal beta-Actin Antibody (AC-15) [HRP]

catalog :

NB600-501H

quantity :

0.1 ml

price :

399 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

AC-15

reactivity :

human, mouse, rat, dog, chicken, cow, pig

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 6	Pastore N, Brady O, Diab H, Martina J, Sun L, Huynh T, et al. TFEB and TFE3 cooperate in the regulation of the innate immune response in activated macrophages. Autophagy. 2016;12:1240-58 pubmed publisher
western blot; mouse; fig 1	Lin C, Chen Y, Lin C, Chen Y, Lo G, Lee P, et al. Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy. Sci Rep. 2015;5:15807 pubmed publisher
western blot; human; fig 6	Kao Y, Chang B, Liang J, Tsai H, Lee Y, Lin R, et al. Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid Î²-oxidation. PLoS Pathog. 2015;11:e1004750 pubmed publisher
western blot; mouse	Vande Walle L, Van Opdenbosch N, Jacques P, Fossoul A, Verheugen E, Vogel P, et al. Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis. Nature. 2014;512:69-73 pubmed publisher
western blot; pig	Brugger D, Buffler M, Windisch W. Development of an experimental model to assess the bioavailability of zinc in practical piglet diets. Arch Anim Nutr. 2014;68:73-92 pubmed publisher
western blot; rat	Liu L, Martin R, Kohler G, Chan C. Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes. Exp Neurol. 2013;248:482-90 pubmed publisher
	Van Opdenbosch N, Gurung P, Vande Walle L, Fossoul A, Kanneganti T, Lamkanfi M. Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation. Nat Commun. 2014;5:3209 pubmed publisher

product information

Catalog Number :

NB600-501H

Product Name :

Mouse Monoclonal beta-Actin Antibody (AC-15) [HRP]

List Price :

399 USD

product type :

Primary Antibodies

Package Size :

0.1 ml

AltNames :

ACTB, actin, beta, actin, cytoplasmic 1, Beta Actin, beta cytoskeletal actin, Beta-actin, BRWS1, PS1TP5-binding protein 1, PS1TP5BP1

Buffer :

PBS

Clonality :

Monoclonal

Clone :

AC-15

Conjugate :

HRP

Application Summary :

ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot

Epitope :

N-terminus of the beta isoform of actin.

Host :

Mouse

Immunogen :

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Isotype :

IgG1

Preservative :

No Preservative

Purity :

Ascites

CrossReactivity :

Bovine, Canine, Chicken, Drosophila (Negative), Feline, Fish, Guinea Pig, Hamster, Human, Leech, Mammal, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Squirrel, Zebrafish

Specificity :

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

Storage :

Store at 4C in the dark.

Swissprot Human :

P60709

TMW :

Uses :

This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. Molecular weight of beta actin is 42kD. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is not suitable for formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives.

Research Area :

Cellular Markers, Cytoskeleton Markers, Loading Controls, Signal Transduction