product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Collagen I alpha 1 Antibody (COL-1)
catalog :
NB600-450
quantity :
0.1 ml (also 0.02 ml)
price :
469 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
COL-1
reactivity :
human, mouse, rat, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, dot blot
more info or order :
citations: 61
Published Application/Species/Sample/DilutionReference
  • proximity ligation assay; human; 1:250; loading ...; fig 2e
  • western blot; mouse; 1:1000; loading ...; fig 6c
Ramani K, Mavila N, Abeynayake A, Tomasi M, Wang J, Matsuda M, et al. Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse models. elife. 2022;11: pubmed publisher
Kim Y, Ghim M, Quan M, Kim Y, Cho Y. Experimental Verification of the Impact of the Contact Area between the Defect Site and the Scaffold on Bone Regeneration Efficacy. Polymers (Basel). 2024;16: pubmed publisher
Shabani Z, Schuerger J, Zhu X, Tang C, Ma L, Yadav A, et al. Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells. 2024;13: pubmed publisher
Beaumont R, Smith E, Zhou L, Marr N, Thorpe C, Guest D. Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression in three-dimensional culture which can be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signaling. Mol Cell Biochem. 2024;479:1059-1078 pubmed publisher
Shigeta Y, Saleh T, Benedetti G, Caciolli L, Chang J, Zambaiti E, et al. Stomach engineering: region-specific characterization of the decellularized porcine stomach. Pediatr Surg Int. 2023;40:13 pubmed publisher
Samra T, Gomez Gomez T, Linowiecka K, Akhundlu A, Lopez de Mendoza G, Gompels M, et al. Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo. Int J Mol Sci. 2023;24: pubmed publisher
Lu G, Li X, Wang P, Li X, Wang Y, Zhu J, et al. Polysaccharide-Based Composite Hydrogel with Hierarchical Microstructure for Enhanced Vascularization and Skull Regeneration. Biomacromolecules. 2023;24:4970-4988 pubmed publisher
Ha H, Lee C, Lee K, Lee K, Park J, Kim S, et al. Shape-Configurable Mesh for Hernia Repair by Synchronizing Anisotropic Body Motion. Small. 2023;:e2303325 pubmed publisher
Simon K, Coelho L, Veloso P, Melo Silva C, Morais J, Longo J, et al. Innovative Pre-Clinical Data Using Peptides to Intervene in the Evolution of Pulmonary Fibrosis. Int J Mol Sci. 2023;24: pubmed publisher
Hazari Y, Urra H, Garcia Lopez V, Diaz J, Tamburini G, Milani M, et al. The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis. bioRxiv. 2023;: pubmed publisher
Huynh T, Reed C, Blackwell Z, Phelps P, Herrera L, Almodóvar J, et al. Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury. Sci Rep. 2023;13:1983 pubmed publisher
Spiaggia G, Taladriz Blanco P, Hengsberger S, Septiadi D, Geers C, Lee A, et al. A Near-Infrared Mechanically Switchable Elastomeric Film as a Dynamic Cell Culture Substrate. Biomedicines. 2022;11: pubmed publisher
Li H, Zhao T, Cao F, Deng H, He S, Li J, et al. Integrated bioactive scaffold with aptamer-targeted stem cell recruitment and growth factor-induced pro-differentiation effects for anisotropic meniscal regeneration. Bioeng Transl Med. 2022;7:e10302 pubmed publisher
Lena H, David G, Johann K, Yvonne J, Frank G Z, Daniela H, et al. In situ regeneration of nasal septal defects using acellular cartilage enhanced with platelet-derived growth factor. J Tissue Eng. 2022;13:20417314221114423 pubmed publisher
Yang C, Zhu C, Li Y, Li Z, Zhang Z, Xu J, et al. Injectable selenium-containing polymeric hydrogel formulation for effective treatment of myocardial infarction. Front Bioeng Biotechnol. 2022;10:912562 pubmed publisher
McClellan P, Ina J, Knapik D, Isali I, Learn G, Valente A, et al. Mesenchymal Stem Cell Delivery via Topographically Tenoinductive Collagen Biotextile Enhances Regeneration of Segmental Tendon Defects. Am J Sports Med. 2022;50:2281-2291 pubmed publisher
Gan Z, Zhao Y, Wu Y, Yang W, Zhao Z, Zhao L. Three-dimensional, biomimetic electrospun scaffolds reinforced with carbon nanotubes for temporomandibular joint disc regeneration. Acta Biomater. 2022;147:221-234 pubmed publisher
Verma S, Yaghoobi H, Slaine P, Baldwin S, Rainey J, Kreplak L, et al. Multi-pin contact drawing enables production of anisotropic collagen fiber substrates for alignment of fibroblasts and monocytes. Colloids Surf B Biointerfaces. 2022;215:112525 pubmed publisher
Kim J, Roberts K, Dunlap G, Perry R, Washington T, Wolchok J. Nandrolone supplementation does not improve functional recovery in an aged animal model of volumetric muscle loss injury. J Tissue Eng Regen Med. 2022;16:367-379 pubmed publisher
Gundogdu G, Okhunov Z, Starek S, Veneri F, Orabi H, Holzman S, et al. Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model. Front Bioeng Biotechnol. 2021;9:791119 pubmed publisher
Struijk C, Van Genechten W, Verdonk P, Krych A, Dietz A, Van Wijnen A, et al. Human meniscus allograft augmentation by allogeneic mesenchymal stromal/stem cell injections. J Orthop Res. 2021;: pubmed publisher
Wei Q, Kong N, Liu X, Tian R, Jiao M, Li Y, et al. Pirfenidone attenuates synovial fibrosis and postpones the progression of osteoarthritis by anti-fibrotic and anti-inflammatory properties in vivo and in vitro. J Transl Med. 2021;19:157 pubmed publisher
Kartasheva Ebertz D, Gaston J, Lair Mehiri L, Massault P, Scatton O, Vaillant J, et al. Adult human liver slice cultures: Modelling of liver fibrosis and evaluation of new anti-fibrotic drugs. World J Hepatol. 2021;13:187-217 pubmed publisher
Kuzan A, Wisniewski J, Maksymowicz K, Kobielarz M, Gamian A, Chwilkowska A. Relationship between calcification, atherosclerosis and matrix proteins in the human aorta. Folia Histochem Cytobiol. 2021;59:8-21 pubmed publisher
Meng X, Grad S, Wen C, Lai Y, Alini M, Qin L, et al. An impaired healing model of osteochondral defect in papain-induced arthritis. J Orthop Translat. 2021;26:101-110 pubmed publisher
Marvin K, Schwartz I, Utz E, Wilson J, Johnson C, Gaudreau P. Effects of Fractional CO2 Laser Treatment on Subglottic Scar in a Rabbit Model. Otolaryngol Head Neck Surg. 2020;:194599820978256 pubmed publisher
Oikawa K, Teixeira L, Keikhosravi A, Eliceiri K, McLellan G. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans. Exp Eye Res. 2020;:108315 pubmed publisher
Huynh T, Kim J, Dunlap G, Ahmadi S, Wolchok J. In vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration. J Shoulder Elbow Surg. 2020;: pubmed publisher
Ma C, Yang D, Wang B, Wu C, Wu Y, Li S, et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation. Sci Adv. 2020;6:eaaz6717 pubmed publisher
Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, et al. Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs. Stem Cells Int. 2020;2020:7082679 pubmed publisher
Liu J, Yu C, Chen Y, Cai H, Lin H, Sun Y, et al. Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture. J Mater Chem B. 2017;5:9130-9140 pubmed publisher
Yu C, Liu J, Lu G, Xie Y, Sun Y, Wang Q, et al. Repair of osteochondral defects in a rabbit model with artificial cartilage particulates derived from cultured collagen-chondrocyte microspheres. J Mater Chem B. 2018;6:5164-5173 pubmed publisher
Zwaans B, Wegner K, Bartolone S, Vezina C, Chancellor M, Lamb L. Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice. Physiol Rep. 2020;8:e14377 pubmed publisher
Rybinska I, Sandri M, Bianchi F, Orlandi R, De Cecco L, Gasparini P, et al. Extracellular Matrix Features Discriminate Aggressive HER2-Positive Breast Cancer Patients Who Benefit from Trastuzumab Treatment. Cells. 2020;9: pubmed publisher
Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, et al. In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells. Int J Mol Sci. 2020;21: pubmed publisher
Willemse J, Verstegen M, Vermeulen A, Schurink I, Roest H, van der Laan L, et al. Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion. Mater Sci Eng C Mater Biol Appl. 2020;108:110200 pubmed publisher
Roßbach B, Gülecyüz M, Kempfert L, Pietschmann M, Ullamann T, Ficklscherer A, et al. Rotator Cuff Repair With Autologous Tenocytes and Biodegradable Collagen Scaffold: A Histological and Biomechanical Study in Sheep. Am J Sports Med. 2020;48:450-459 pubmed publisher
Chen M, Feng Z, Guo W, Yang D, Gao S, Li Y, et al. PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model. ACS Appl Mater Interfaces. 2019;11:41626-41639 pubmed publisher
Jallow F, O Leary K, Rugowski D, Guerrero J, Ponik S, Schuler L. Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer. Oncogene. 2019;38:6913-6925 pubmed publisher
Wada H, Ikoma K, Oka Y, Nishida A, Onishi O, Kim W, et al. Status of growth plates can be monitored by MRI. J Magn Reson Imaging. 2019;: pubmed publisher
Wu D, Wang Z, Wang J, Geng Y, Zhang Z, Li Y, et al. Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment. Stem Cell Res Ther. 2018;9:331 pubmed publisher
Learn G, McClellan P, Knapik D, Cumsky J, Webster Wood V, Anderson J, et al. Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo. J Biomed Mater Res B Appl Biomater. 2019;107:1864-1876 pubmed publisher
Costa D, Vené R, Benelli R, Romairone E, Scabini S, Catellani S, et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts. Front Immunol. 2018;9:1150 pubmed publisher
Kuzan A, Chwiłkowska A, Maksymowicz K, Bronowicka Szydełko A, Stach K, Pezowicz C, et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods. Glycoconj J. 2018;35:95-103 pubmed publisher
Ramani K, Tomasi M, Berlind J, Mavila N, Sun Z. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. Am J Pathol. 2018;188:640-655 pubmed publisher
Liu G, Agarwal R, Ko K, Ruthven M, Sarhan H, Frampton J. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Sci Rep. 2017;7:9628 pubmed publisher
Wegner K, Keikhosravi A, Eliceiri K, Vezina C. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem. 2017;65:479-490 pubmed publisher
da Silva I, Tiveron L, da Silva M, Peixoto A, Carneiro C, dos Reis M, et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm. 2017;2017:6573802 pubmed publisher
Du Y, Liu H, Yang Q, Wang S, Wang J, Ma J, et al. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials. 2017;137:37-48 pubmed publisher
Bono N, Meghezi S, Soncini M, Piola M, Mantovani D, Fiore G. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017;45:1496-1510 pubmed publisher
Sa G, Xiong X, Ren J, Zhao Y, He S. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci. 2017;96:472-478 pubmed publisher
Karti O, Zengin M, Cinar E, Tutuncu M, Karahan E, Celik A, et al. Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations. Cornea. 2016;35:1644-1649 pubmed publisher
Loy C, Lainé A, Mantovani D. Rotation-based technique for the rapid densification of tubular collagen gel scaffolds. Biotechnol J. 2016;11:1673-1679 pubmed publisher
Cheng M, Wahafu T, Jiang G, Liu W, Qiao Y, Peng X, et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016;6:24134 pubmed publisher
Geburek F, Lietzau M, Beineke A, Rohn K, Stadler P. Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies. Stem Cell Res Ther. 2015;6:126 pubmed publisher
Otani Y, Komura M, Komura H, Ishimaru T, Konishi K, Komuro H, et al. Optimal amount of basic fibroblast growth factor in gelatin sponges incorporating β-tricalcium phosphate with chondrocytes. Tissue Eng Part A. 2015;21:627-36 pubmed publisher
Triulzi T, Ratti M, Tortoreto M, Ghirelli C, Aiello P, Regondi V, et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2014;134:2789-97 pubmed publisher
You W, Fan L, Duan D, Tian L, Dang X, Wang C, et al. Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation. Mol Cell Biochem. 2014;386:125-34 pubmed
Murali S, Rai B, Dombrowski C, Lee J, Lim Z, Bramono D, et al. Affinity-selected heparan sulfate for bone repair. Biomaterials. 2013;34:5594-605 pubmed publisher
Re em T, Kaminer Israeli Y, Ruvinov E, Cohen S. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds. Biomaterials. 2012;33:751-61 pubmed publisher
Krasinski Z, Biskupski P, Dzieciuchowicz Ł, Kaczmarek E, Krasinska B, Staniszewski R, et al. The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction. Eur J Vasc Endovasc Surg. 2010;40:224-9 pubmed publisher
image
image 1 :
Novus Biologicals NB600-450 image 1
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
product information
brand :
Novus Biologicals, a Bio-Techne brand
catalog number base :
NB600-450
SKU :
NB600-450
product name :
Collagen I alpha 1 Antibody (COL-1)
description :
The Collagen I alpha 1 Antibody (COL-1) from Novus Biologicals is a mouse monoclonal antibody to Collagen I alpha 1. This antibody reacts with bovine,equine,feline,human,mammal,mouse,porcine,rabbit,rat. The Collagen I alpha 1 Antibody (COL-1) has been validated for the following applications: Dot Blot,IF/IHC,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Electron Microscopy,Immunocytochemistry/ Immunofluorescence.
target :
Collagen I alpha 1
unit size :
0.1 ml (also 0.02 ml)
category :
Primary Antibodies
buffer :
Ascites
clonality :
Monoclonal
clone :
COL-1
conjugate :
Unconjugated
host :
Mouse
immunogen :
This Collagen I alpha 1 antibody (COL-1) was raised against full length bovine native protein (purified).
isotype :
IgG1
purity :
Unpurified
species :
Bovine,Equine,Feline,Human,Mammal,Mouse,Porcine,Rabbit,Rat
specificity :
This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
theoretical molecular weight :
139 kDa
gene symbol :
COL1A1
applications :
Dot Blot,IF/IHC,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Electron Microscopy,Immunocytochemistry/ Immunofluorescence
USD :
449
USD 2025 :
469 USD
alt names :
Alpha-1 type I collagen, COL-IA1, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, Collagen Type I Alpha 1 Chain, collagen, type I, alpha 1, Collagen1, Collagen-1, EDSARTH1, OI4, pro-alpha-1 collagen type 1, Type I Procollagen Alpha 1 Chain
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.