Luciferase Antibody (Luci 21 1-107) | Novus Biologicals NB600-307 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Luciferase Antibody (Luci 21 1-107)

catalog :

NB600-307

quantity :

0.1 ml (also 0.025 ml)

price :

349 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Luci 21 1-107

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Novus Biologicals product webpage

citations: 54

Published Application/Species/Sample/Dilution	Reference
western blot; rat western blot; mouse	Sayed D, Yang Z, He M, Pfleger J, Abdellatif M. Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription. Circ Heart Fail. 2015;8:138-48 pubmed publisher
immunocytochemistry; mouse	Schmeisser M, Baumann B, Johannsen S, Vindedal G, Jensen V, Hvalby Ø, et al. I?B kinase/nuclear factor ?B-dependent insulin-like growth factor 2 (Igf2) expression regulates synapse formation and spine maturation via Igf2 receptor signaling. J Neurosci. 2012;32:5688-703 pubmed publisher
immunohistochemistry; mouse	Kawabata A, Baoum A, Ohta N, Jacquez S, Seo G, Berkland C, et al. Intratracheal administration of a nanoparticle-based therapy with the angiotensin II type 2 receptor gene attenuates lung cancer growth. Cancer Res. 2012;72:2057-67 pubmed publisher
	Cheng Y, Chiang H, Cheng S, Chang H, Li Y, Shieh S. Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation. Cell Death Dis. 2020;11:1046 pubmed publisher
	Korgaonkar A, Nguyen S, Li Y, Sekhar D, Subramanian D, Guevarra J, et al. Distinct cellular mediators drive the Janus faces of toll-like receptor 4 regulation of network excitability which impacts working memory performance after brain injury. Brain Behav Immun. 2020;88:381-395 pubmed publisher
	Chen Z, Yao L, Liu Y, Pan Z, Peng S, Wan G, et al. Astragaloside IV regulates NF-κB-mediated cellular senescence and apoptosis of hepatic stellate cells to suppress PDGF-BB-induced activation. Exp Ther Med. 2019;18:3741-3750 pubmed publisher
	Galisova A, Herynek V, Swider E, Sticová E, Pátiková A, Kosinová L, et al. A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging. Mol Imaging Biol. 2019;21:454-464 pubmed publisher
	Chitwood C, Dietzsch C, Jacobs G, McArdle T, Freeman B, Banga A, et al. Breast tumor cell hybrids form spontaneously in vivo and contribute to breast tumor metastases. APL Bioeng. 2018;2:031907 pubmed publisher
	Shteingauz A, Porat Y, Voloshin T, Schneiderman R, Munster M, Zeevi E, et al. AMPK-dependent autophagy upregulation serves as a survival mechanism in response to Tumor Treating Fields (TTFields). Cell Death Dis. 2018;9:1074 pubmed publisher
	Cunningham D, Zhang Q, Liu S, Parajuli K, Nie Q, Ma L, et al. Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer. Am J Clin Exp Urol. 2018;6:114-122 pubmed
	Zhu F, Willette Brown J, Song N, Lomada D, Song Y, Xue L, et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. 2017;21:478-493.e7 pubmed publisher
	Tomasi M, Ramani K, Ryoo M. Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response. Am J Pathol. 2016;186:2326-36 pubmed publisher
	Gao Q, Liu Y, Wu Y, Zhao Q, Wang L, Gao S, et al. IL-17 intensifies IFN-Î³-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-ÎºB. Int J Mol Med. 2016;37:347-58 pubmed publisher
	Hochrainer K, Pejanovic N, Olaseun V, Zhang S, Iadecola C, Anrather J. The ubiquitin ligase HERC3 attenuates NF-ÎºB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation. Nucleic Acids Res. 2015;43:9889-904 pubmed publisher
	Ikeno H, Apel M, Zouboulis C, Luger T, BÃ¶hm M. L-Ascorbyl-2-phosphate attenuates NF-ÎºB signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion. Arch Dermatol Res. 2015;307:595-605 pubmed publisher
	Wang K, Liu S, Wang J, Wu Y, Cai F, Song W. Transcriptional regulation of human USP24 gene expression by NF-kappa B. J Neurochem. 2014;128:818-28 pubmed publisher
	Yang X, Zhao N, Liu Y, Hu B, Sun K, Chang X, et al. Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury. Evid Based Complement Alternat Med. 2013;2013:728020 pubmed publisher
	Tabit C, Shenouda S, Holbrook M, Fetterman J, Kiani S, Frame A, et al. Protein kinase C-? contributes to impaired endothelial insulin signaling in humans with diabetes mellitus. Circulation. 2013;127:86-95 pubmed publisher
	Pejanovic N, Hochrainer K, Liu T, Aerne B, Soares M, Anrather J. Regulation of nuclear factor ?B (NF-?B) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT). PLoS ONE. 2012;7:e42020 pubmed publisher
	Delhase M, Kim S, Lee H, Naiki Ito A, Chen Y, Ahn E, et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012;109:E177-86 pubmed publisher
	Li J, Bush J, Xiong Y, Li L, McCormack M. Large-scale protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS ONE. 2011;6:e27364 pubmed publisher
	Harikumar K, Sung B, Pandey M, Guha S, Krishnan S, Aggarwal B. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010;77:818-27 pubmed publisher
	Iijima K, Zhao L, Shenton C, Iijima Ando K. Regulation of energy stores and feeding by neuronal and peripheral CREB activity in Drosophila. PLoS ONE. 2009;4:e8498 pubmed publisher
	Yu M, Su L, Zou L, Liu Y, Wu N, Kong L, et al. An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells. Nat Immunol. 2008;9:898-907 pubmed publisher
	Nishioka C, Ikezoe T, Jing Y, Umezawa K, Yokoyama A. DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dendritic ce. Immunology. 2008;124:198-205 pubmed publisher
	Chinta S, Kumar M, Hsu M, Rajagopalan S, Kaur D, Rane A, et al. Inducible alterations of glutathione levels in adult dopaminergic midbrain neurons result in nigrostriatal degeneration. J Neurosci. 2007;27:13997-4006 pubmed
	Love Z, Wang F, Dennis J, Awadallah A, Salem N, Lin Y, et al. Imaging of mesenchymal stem cell transplant by bioluminescence and PET. J Nucl Med. 2007;48:2011-20 pubmed
	Wilber A, Linehan J, Tian X, Woll P, Morris J, Belur L, et al. Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer. Stem Cells. 2007;25:2919-27 pubmed
	Shakibaei M, John T, Schulze Tanzil G, Lehmann I, Mobasheri A. Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis. Biochem Pharmacol. 2007;73:1434-45 pubmed
	Tang X, Molina M, Amar S. p53 short peptide (p53pep164) regulates lipopolysaccharide-induced tumor necrosis factor-alpha factor/cytokine expression. Cancer Res. 2007;67:1308-16 pubmed
	Vilimas T, Mascarenhas J, Palomero T, Mandal M, Buonamici S, Meng F, et al. Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia. Nat Med. 2007;13:70-7 pubmed
	Endo T, Nishio M, Enzler T, Cottam H, Fukuda T, James D, et al. BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway. Blood. 2007;109:703-10 pubmed
	Ishiguro K, Ando T, Maeda O, Hasegawa M, Kadomatsu K, Ohmiya N, et al. Paeonol attenuates TNBS-induced colitis by inhibiting NF-kappaB and STAT1 transactivation. Toxicol Appl Pharmacol. 2006;217:35-42 pubmed
	Aleksic T, Baumann B, Wagner M, Adler G, Wirth T, Weber C. Cellular immune reaction in the pancreas is induced by constitutively active IkappaB kinase-2. Gut. 2007;56:227-36 pubmed
	Santhi W, Sebastian P, Varghese B, Prakash O, Pillai M. NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins. Exp Mol Pathol. 2006;81:123-30 pubmed
	Macagno A, Molteni M, Rinaldi A, Bertoni F, Lanzavecchia A, Rossetti C, et al. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression. J Exp Med. 2006;203:1481-92 pubmed
	Strozyk E, Poppelmann B, Schwarz T, Kulms D. Differential effects of NF-kappaB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome. Oncogene. 2006;25:6239-51 pubmed
	Zhou W, Song W. Leaky scanning and reinitiation regulate BACE1 gene expression. Mol Cell Biol. 2006;26:3353-64 pubmed
	Perez Moreno M, Davis M, Wong E, Pasolli H, Reynolds A, Fuchs E. p120-catenin mediates inflammatory responses in the skin. Cell. 2006;124:631-44 pubmed
	Chang L, Kamata H, Solinas G, Luo J, Maeda S, Venuprasad K, et al. The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover. Cell. 2006;124:601-13 pubmed
	Yang M, Hase H, Legarda Addison D, Varughese L, Seed B, Ting A. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. J Immunol. 2005;175:2814-24 pubmed
	Napolitani G, Rinaldi A, Bertoni F, Sallusto F, Lanzavecchia A. Selected Toll-like receptor agonist combinations synergistically trigger a T helper type 1-polarizing program in dendritic cells. Nat Immunol. 2005;6:769-76 pubmed
	Bernasconi D, Amici C, La Frazia S, Ianaro A, Santoro M. The IkappaB kinase is a key factor in triggering influenza A virus-induced inflammatory cytokine production in airway epithelial cells. J Biol Chem. 2005;280:24127-34 pubmed
	Xia Y, Ye B, Li Y, Wang J, He X, Lin X, et al. Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50. J Immunol. 2004;173:4207-17 pubmed
	Saccani S, Marazzi I, Beg A, Natoli G. Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response. J Exp Med. 2004;200:107-13 pubmed
	Ikezoe T, Yang Y, Heber D, Taguchi H, Koeffler H. PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock. Mol Pharmacol. 2003;64:1521-9 pubmed
	Petrescu A, Payne H, Boedecker A, Chao H, Hertz R, Bar Tana J, et al. Physical and functional interaction of Acyl-CoA-binding protein with hepatocyte nuclear factor-4 alpha. J Biol Chem. 2003;278:51813-24 pubmed
	Wang X, Rosol M, Ge S, Peterson D, McNamara G, Pollack H, et al. Dynamic tracking of human hematopoietic stem cell engraftment using in vivo bioluminescence imaging. Blood. 2003;102:3478-82 pubmed
	Saccani S, Pantano S, Natoli G. Modulation of NF-kappaB activity by exchange of dimers. Mol Cell. 2003;11:1563-74 pubmed
	Goodkin M, Ting A, Blaho J. NF-kappaB is required for apoptosis prevention during herpes simplex virus type 1 infection. J Virol. 2003;77:7261-80 pubmed
	Han H, Karabiyikoglu M, Kelly S, Sobel R, Yenari M. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003;23:589-98 pubmed
	Delhase M. IkappaB kinase and NF-kappaB signaling in response to pro-inflammatory cytokines. Methods Mol Biol. 2003;225:7-17 pubmed
	Saccani S, Pantano S, Natoli G. p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment. Nat Immunol. 2002;3:69-75 pubmed
	Saccani S, Pantano S, Natoli G. Two waves of nuclear factor kappaB recruitment to target promoters. J Exp Med. 2001;193:1351-9 pubmed

image

image 1 :

Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573)

product information

brand :

Novus

SKU :

NB600-307

product name :

Luciferase Antibody (Luci 21 1-107)

unit sizes :

0.1 ml (also 0.025 ml)

description :

The Luciferase Antibody (Luci 21 1-107) from Novus is a mouse monoclonal antibody to Luciferase. This antibody reacts with firefly. The Luciferase Antibody (Luci 21 1-107) has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

target :

Luciferase

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

Luci 21 1-107

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

This Luciferase Antibody (Luci 21 1-107) was developed against luciferase protein from Photinus pyralis (North American firefly). [UniProt# P08659].

isotype :

IgG1 Kappa

purity :

Protein A or G purified

species :

Firefly

specificity :

This Luciferase Antibody (Luci 21 1-107) is specific for Luciferase, recognizing a peptide consisting of the first 258 amino acids

storage :

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

uses :

Western blot has been tested with Drosophila embryos. Purified Luciferase protein and Luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kDa, representing Luciferase. Flow Cytometry has been reported in a customer review. Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 31069316).

theoretical molecular weight :

61 kDa

catalog number base :

NB600-307

accessionNumbers :

P08659

applications :

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

USD :

349 USD

default size :

TRUE

alt names :

ec 1.13.12.7, firefly luciferase, Fluc, LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase

more info or order :

Novus Biologicals product webpage