product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free
catalog :
NB600-235
quantity :
0.1 ml (also 0.025 ml)
price :
429 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3H2-E8
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Ye Y, Liu M, Tang L, Du F, Liu Y, Hao P, et al. Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis. Arthritis Res Ther. 2019;21:92 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s7a
Cao Y, Trillo Tinoco J, Sierra R, Anadon C, Dai W, Mohamed E, et al. ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression. Nat Commun. 2019;10:1280 pubmed publisher
  • flow cytometry; mouse
  • western blot; mouse
Jang K, Mano H, Aoki K, Hayashi T, Muto A, Nambu Y, et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates. Nat Commun. 2015;6:6750 pubmed publisher
  • western blot; human
Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, et al. The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE. 2014;9:e106008 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
  • flow cytometry; mouse
  • immunocytochemistry; mouse
Kim J, Nakasaki M, Todorova D, Lake B, Yuan C, Jamora C, et al. p53 Induces skin aging by depleting Blimp1+ sebaceous gland cells. Cell Death Dis. 2014;5:e1141 pubmed publisher
  • western blot; human
Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, et al. DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. Dev Cell. 2014;28:697-710 pubmed publisher
  • western blot; mouse
Lin M, Yeh L, Chen S, Chiou H, Chu C, Yen L, et al. T cell-specific BLIMP-1 deficiency exacerbates experimental autoimmune encephalomyelitis in nonobese diabetic mice by increasing Th1 and Th17 cells. Clin Immunol. 2014;151:101-13 pubmed publisher
  • western blot; mouse
Severa M, Islam S, Waggoner S, Jiang Z, Kim N, Ryan G, et al. The transcriptional repressor BLIMP1 curbs host defenses by suppressing expression of the chemokine CCL8. J Immunol. 2014;192:2291-304 pubmed publisher
  • flow cytometry; mouse
Kim E, Gasper D, Lee S, Plisch E, Svaren J, Suresh M. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J Immunol. 2014;192:985-95 pubmed publisher
  • immunohistochemistry - paraffin section; human
Wong L, Brulois K, Toth Z, Inn K, Lee S, O Brien K, et al. The product of Kaposi's sarcoma-associated herpesvirus immediate early gene K4.2 regulates immunoglobulin secretion and calcium homeostasis by interacting with and inhibiting pERP1. J Virol. 2013;87:12069-79 pubmed publisher
  • western blot; human
Parlato S, Bruni R, Fragapane P, Salerno D, Marcantonio C, Borghi P, et al. IFN-? regulates Blimp-1 expression via miR-23a and miR-125b in both monocytes-derived DC and pDC. PLoS ONE. 2013;8:e72833 pubmed publisher
  • western blot; mouse
Lin M, Chou F, Yeh L, Fu S, Chiou H, Lin K, et al. B lymphocyte-induced maturation protein 1 (BLIMP-1) attenuates autoimmune diabetes in NOD mice by suppressing Th1 and Th17 cells. Diabetologia. 2013;56:136-46 pubmed publisher
  • western blot; mouse
Lee S, Rutan J, Monteith A, Jones S, Kang S, Krum K, et al. Receptor cross-talk spatially restricts p-ERK during TLR4 stimulation of autoreactive B cells. J Immunol. 2012;189:3859-68 pubmed publisher
  • immunohistochemistry; human
Takata K, Sato Y, Nakamura N, Tokunaka M, Miki Y, Yukie Kikuti Y, et al. Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics. Mod Pathol. 2013;26:22-31 pubmed publisher
  • immunoprecipitation; human
Morgan M, Mould A, Li L, Robertson E, Bikoff E. Alternative splicing regulates Prdm1/Blimp-1 DNA binding activities and corepressor interactions. Mol Cell Biol. 2012;32:3403-13 pubmed publisher
  • western blot; human
Ramon S, Gao F, Serhan C, Phipps R. Specialized proresolving mediators enhance human B cell differentiation to antibody-secreting cells. J Immunol. 2012;189:1036-42 pubmed publisher
  • western blot; human
Le Gallou S, Caron G, Delaloy C, Rossille D, Tarte K, Fest T. IL-2 requirement for human plasma cell generation: coupling differentiation and proliferation by enhancing MAPK-ERK signaling. J Immunol. 2012;189:161-73 pubmed publisher
  • flow cytometry; mouse
Altin J, Goodnow C, Cook M. IL-10+ CTLA-4+ Th2 inhibitory cells form in a Foxp3-independent, IL-2-dependent manner from Th2 effectors during chronic inflammation. J Immunol. 2012;188:5478-88 pubmed publisher
  • western blot; human
Nagy N, Adori M, Rasul A, Heuts F, Salamon D, Ujvári D, et al. Soluble factors produced by activated CD4+ T cells modulate EBV latency. Proc Natl Acad Sci U S A. 2012;109:1512-7 pubmed publisher
  • immunoprecipitation; mouse
Shimshon L, Michaeli A, Hadar R, Nutt S, David Y, Navon A, et al. SUMOylation of Blimp-1 promotes its proteasomal degradation. FEBS Lett. 2011;585:2405-9 pubmed publisher
  • immunocytochemistry; human
Julaton V, Reijo Pera R. NANOS3 function in human germ cell development. Hum Mol Genet. 2011;20:2238-50 pubmed publisher
  • immunohistochemistry - frozen section; human
  • immunohistochemistry - paraffin section; human
Courts C, Montesinos Rongen M, Brunn A, Bug S, Siemer D, Hans V, et al. Recurrent inactivation of the PRDM1 gene in primary central nervous system lymphoma. J Neuropathol Exp Neurol. 2008;67:720-7 pubmed publisher
  • immunocytochemistry; mouse
Robertson E, Charatsi I, Joyner C, Koonce C, Morgan M, Islam A, et al. Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development. 2007;134:4335-45 pubmed
  • western blot; mouse
John S, Clements J, Russell L, Garrett Sinha L. Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1. J Biol Chem. 2008;283:951-62 pubmed
  • immunohistochemistry - paraffin section; human
  • western blot; human
Liu Y, Leboeuf C, Shi J, Li J, Wang L, Shen Y, et al. Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma. Blood. 2007;110:339-44 pubmed
  • immunohistochemistry - paraffin section; human
  • western blot; human
Cattoretti G, Angelin Duclos C, Shaknovich R, Zhou H, Wang D, Alobeid B. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage. J Pathol. 2005;206:76-86 pubmed
  • immunohistochemistry - paraffin section; mouse
Chang D, Cattoretti G, Calame K. The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech Dev. 2002;117:305-9 pubmed
Tan K, Wilkinson M. Regulation of both transcription and RNA turnover contribute to germline specification. Nucleic Acids Res. 2022;50:7310-7325 pubmed publisher
Nishikawa K, Seno S, Yoshihara T, Narazaki A, Sugiura Y, Shimizu R, et al. Osteoclasts adapt to physioxia perturbation through DNA demethylation. EMBO Rep. 2021;22:e53035 pubmed publisher
Dong G, Li Y, Lee L, Liu X, Shi Y, Liu X, et al. Genetic manipulation of primary human natural killer cells to investigate the functional and oncogenic roles of PRDM1. Haematologica. 2020;: pubmed publisher
Wang Y, Wang C, Cai X, Mou C, Cui X, Zhang Y, et al. IL-21 Stimulates the expression and activation of cell cycle regulators and promotes cell proliferation in EBV-positive diffuse large B cell lymphoma. Sci Rep. 2020;10:12326 pubmed publisher
Iwanaga R, Truong B, Hsu J, Lambert K, Vyas R, Orlicky D, et al. Loss of prdm1a accelerates melanoma onset and progression. Mol Carcinog. 2020;59:1052-1063 pubmed publisher
Meer S, Perner Y, McAlpine E, Willem P. Extraoral plasmablastic lymphomas in a high human immunodeficiency virus endemic area. Histopathology. 2020;76:212-221 pubmed publisher
Hussaini M, Yeung C, Zhu X, Mirza A, Pfeifer J, Hassan A. PRDM1 expression levels in marginal zone lymphoma and lymphoplasmacytic lymphoma. Int J Clin Exp Pathol. 2017;10:8610-8618 pubmed
Tian Y, Seumois G, De Oliveira Pinto L, Mateus J, Herrera de la Mata S, Kim C, et al. Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease. Cell Rep. 2019;29:4482-4495.e4 pubmed publisher
Wu L, Ehlin Henriksson B, Zhou X, Zhu H, Ernberg I, Kis L, et al. Epstein-Barr virus (EBV) provides survival factors to EBV+ diffuse large B-cell lymphoma (DLBCL) lines and modulates cytokine induced specific chemotaxis in EBV+  DLBCL. Immunology. 2017;152:562-573 pubmed publisher
McCarthy C, Kenny L. Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia. Sci Rep. 2016;6:32683 pubmed publisher
Ramon S, Baker S, Sahler J, Kim N, Feldsott E, Serhan C, et al. The specialized proresolving mediator 17-HDHA enhances the antibody-mediated immune response against influenza virus: a new class of adjuvant?. J Immunol. 2014;193:6031-40 pubmed publisher
Gosal K, Dunlop K, Dhaliwal R, Ivanovska J, Kantores C, Desjardins J, et al. Rho kinase mediates right ventricular systolic dysfunction in rats with chronic neonatal pulmonary hypertension. Am J Respir Cell Mol Biol. 2015;52:717-27 pubmed publisher
Joo H, Li D, Dullaers M, Kim T, Duluc D, Upchurch K, et al. C-type lectin-like receptor LOX-1 promotes dendritic cell-mediated class-switched B cell responses. Immunity. 2014;41:592-604 pubmed publisher
Fan C, Lee L, Yu M, Tzen C, Chou C, Chang M. Upregulated hPuf-A promotes breast cancer tumorigenesis. Tumour Biol. 2013;34:2557-64 pubmed publisher
Gao Y, Kazama H, Yonehara S. Bim regulates B-cell receptor-mediated apoptosis in the presence of CD40 signaling in CD40-pre-activated splenic B cells differentiating into plasma cells. Int Immunol. 2012;24:283-92 pubmed publisher
Maseda D, Smith S, DiLillo D, Bryant J, Candando K, Weaver C, et al. Regulatory B10 cells differentiate into antibody-secreting cells after transient IL-10 production in vivo. J Immunol. 2012;188:1036-48 pubmed publisher
Matsushita T, Tedder T. Identifying regulatory B cells (B10 cells) that produce IL-10 in mice. Methods Mol Biol. 2011;677:99-111 pubmed publisher
Nishikawa K, Nakashima T, Hayashi M, Fukunaga T, Kato S, Kodama T, et al. Blimp1-mediated repression of negative regulators is required for osteoclast differentiation. Proc Natl Acad Sci U S A. 2010;107:3117-22 pubmed publisher
Brzezinski J, Lamba D, Reh T. Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development. Development. 2010;137:619-29 pubmed publisher
Garcia Bates T, Baglole C, Bernard M, Murant T, Simpson Haidaris P, Phipps R. Peroxisome proliferator-activated receptor gamma ligands enhance human B cell antibody production and differentiation. J Immunol. 2009;183:6903-12 pubmed publisher
Wang X, Belguise K, O Neill C, Sánchez Morgan N, Romagnoli M, Eddy S, et al. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol. 2009;29:3832-44 pubmed publisher
Gong D, Malek T. Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production. J Immunol. 2007;178:242-52 pubmed
Severa M, Coccia E, Fitzgerald K. Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1. J Biol Chem. 2006;281:26188-95 pubmed
product information
brand :
Novus
master code :
NB600-235
SKU :
NB600-235
product name :
BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free
description :
The BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free from Novus Biologicals is a mouse monoclonal antibody to BLIMP1/PRDM1. This antibody reacts with human, mouse, porcine. The BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
target :
BLIMP1/PRDM1
category :
Primary Antibodies
sizes available :
0.1 ml (also 0.025 ml)
buffer :
PBS
clonality :
Monoclonal
clone :
3H2-E8
concentration :
1 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
isotype :
IgG1
purity :
Protein G purified
species :
Human, Mouse, Porcine
gene symbol :
PRDM1
applications :
Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
USD :
410
USD 2023 :
429 USD
alt names :
Beta-interferon gene positive regulatory domain I-binding factor, beta-interferon gene positive-regulatory domain I binding factor, BLIMP-1, BLIMP1MGC118925, blmp-1, B-lymphocyte-induced maturation protein 1, MGC118922, MGC118923, Positive regulatory domain I-binding factor 1, PR domain containing 1, with ZNF domain, PR domain zinc finger protein 1, PR domain-containing protein 1, PRDI-BF1MGC118924, PRDI-binding factor 1, PRDI-binding factor-1, PR-domain zinc finger protein 1
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.