PKC alpha Antibody (MC5) | Novus Biologicals NB600-201 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

PKC alpha Antibody (MC5)

catalog :

NB600-201

quantity :

0.1 ml (also 0.025 ml)

price :

379 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MC5

reactivity :

human, mouse, rat, dogs, chicken, bovine, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, radioimmunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Novus Biologicals product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 1g, 3a	Matsuyama T, Tu H, Sun J, Hashiguchi T, Akiba R, Sho J, et al. Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation. iScience. 2021;24:102866 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Bayley P, Morgans C. Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina. J Comp Neurol. 2007;500:286-98 pubmed
immunoprecipitation; human immunocytochemistry; human western blot; human	Ng T, Parsons M, Hughes W, Monypenny J, Zicha D, Gautreau A, et al. Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility. EMBO J. 2001;20:2723-41 pubmed
western blot; rat	Cesare P, Dekker L, Sardini A, Parker P, McNaughton P. Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat. Neuron. 1999;23:617-24 pubmed
western blot; rat	Ricciarelli R, Tasinato A, Clement S, Ozer N, Boscoboinik D, Azzi A. alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state. Biochem J. 1998;334 ( Pt 1):243-9 pubmed
western blot; rat	Gatti A, Robinson P. Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon. Eur J Biochem. 1997;249:92-7 pubmed
western blot; rat	Clement S, Tasinato A, Boscoboinik D, Azzi A. The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation. Eur J Biochem. 1997;246:745-9 pubmed
western blot; rat	Gatti A, Robinson P. Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation. J Biol Chem. 1996;271:31718-22 pubmed
immunocytochemistry; mouse immunocytochemistry; human	Wyss Coray T, Masliah E, Toggas S, Rockenstein E, Brooker M, Lee H, et al. Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis. J Clin Invest. 1996;97:789-98 pubmed
western blot; rat	Escriba P, Sastre M, García Sevilla J. Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures. Proc Natl Acad Sci U S A. 1995;92:7595-9 pubmed
western blot; human	Lacal J, Cuadrado A, Jones J, Trotta R, Burstein D, Thomson T, et al. Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells. Mol Cell Biol. 1990;10:2983-90 pubmed
	Antoniali G, Dalla E, Mangiapane G, Zhao X, Jing X, Cheng Y, et al. APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b. Cell Mol Life Sci. 2022;79:446 pubmed publisher
	Yamasaki S, Tu H, Matsuyama T, Horiuchi M, Hashiguchi T, Sho J, et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. iScience. 2022;25:103657 pubmed publisher
	Della Santina L, Yu A, Harris S, Soli xf1 o M, Garcia Ruiz T, Most J, et al. Disassembly and rewiring of a mature converging excitatory circuit following injury. Cell Rep. 2021;36:109463 pubmed publisher
	Chen A, Biggar K, Nygard K, Singal S, Zhao T, Li C, et al. IGFBP-1 hyperphosphorylation in response to nutrient deprivation is mediated by activation of protein kinase Cα (PKCα). Mol Cell Endocrinol. 2021;536:111400 pubmed publisher
	Thomson B, Grannonico M, Liu F, Liu M, Mendapara P, Xu Y, et al. Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma. Transl Vis Sci Technol. 2020;9:16 pubmed publisher
	Hayward B, Steinbach P, Usdin K. A point mutation in the nuclease domain of MLH3 eliminates repeat expansions in a mouse stem cell model of the Fragile X-related disorders. Nucleic Acids Res. 2020;: pubmed publisher
	Sun H, Yang X, Liang L, Zhang M, Li Y, Chen J, et al. Metabolic switch and epithelial-mesenchymal transition cooperate to regulate pluripotency. EMBO J. 2020;39:e102961 pubmed publisher
	Liu Z, Yao M, Yao H, Hu G, Qin B. Generation of Rybp homozygous knockout murine ES cell line GIBHe001-A-1 by using CRISPR/Cas9 technology. Stem Cell Res. 2019;41:101638 pubmed publisher
	Malik V, Glaser L, Zimmer D, Velychko S, Weng M, Holzner M, et al. Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2. Nat Commun. 2019;10:3477 pubmed publisher
	Grillo S, McDevitt D, Voas M, Khan A, Grillo M, Stella S. Adenosine receptor expression in the adult zebrafish retina. Purinergic Signal. 2019;15:327-342 pubmed publisher
	Lin Y, Liang W, Chen C, Tsang H, Chiou J, Liu X, et al. Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells. Phytomedicine. 2019;58:152893 pubmed publisher
	Castelli A, Susani L, Menale C, Muggeo S, Caldana E, Strina D, et al. Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells. Stem Cells. 2019;37:876-887 pubmed publisher
	Glennon E, Austin L, Arang N, Kain H, Mast F, Vijayan K, et al. Alterations in Phosphorylation of Hepatocyte Ribosomal Protein S6 Control Plasmodium Liver Stage Infection. Cell Rep. 2019;26:3391-3399.e4 pubmed publisher
	Kodama Y, Harinath D, Mihara Tomiyama N, Tominaga N, Ide Y, Nakahara T, et al. Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression. Biochem Biophys Res Commun. 2019;509:1008-1014 pubmed publisher
	Ahmed M, El Sayed A, Chen H, Zhao R, Jin K, Zuo Q, et al. Direct conversion of mouse embryonic fibroblast to osteoblast cells using hLMP-3 with Yamanaka factors. Int J Biochem Cell Biol. 2019;106:84-95 pubmed publisher
	Hellmer C, Clemons M, Nawy S, Ichinose T. A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina. Physiol Rep. 2018;6:e13885 pubmed publisher
	Liu C, Cai Z, Jin G, Peng D, Pan B, Zhang X, et al. Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance. Cell Discov. 2018;4:54 pubmed publisher
	Veerapandian V, Ackermann J, Srivastava Y, Malik V, Weng M, Yang X, et al. Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Stem Cell Reports. 2018;11:593-606 pubmed publisher
	Lee J, Kim M, Park S, Jang Y. Tousled-like kinase 1 is a negative regulator of core transcription factors in murine embryonic stem cells. Sci Rep. 2018;8:334 pubmed publisher
	Zhang N, Lyu Y, Pan X, Xu L, Xuan A, He X, et al. miRâ€‘146bâ€‘5p promotes the neural conversion of pluripotent stem cells by targeting Smad4. Int J Mol Med. 2017;40:814-824 pubmed publisher
	Xie J, Huo S, Li Y, Dai J, Xu H, Yin Z. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the MÃ¼ller Cell Notch Signaling Pathway. Cell Transplant. 2017;26:967-982 pubmed publisher
	Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. 2016;17:94 pubmed publisher
	GuimarÃ£es Souza E, Perche O, Morgans C, Duvoisin R, Calaza K. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Exp Eye Res. 2016;146:72-82 pubmed publisher
	Paulis M, Castelli A, Susani L, Lizier M, Lagutina I, Focarelli M, et al. Chromosome transplantation as a novel approach for correcting complex genomic disorders. Oncotarget. 2015;6:35218-30 pubmed publisher
	Neri T, Muggeo S, Paulis M, Caldana M, Crisafulli L, Strina D, et al. Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts. Stem Cell Reports. 2015;5:558-68 pubmed publisher
	Kim J, Liu Y, Qiu M, Xu Y. Pluripotency factor Nanog is tumorigenic by deregulating DNA damage response in somatic cells. Oncogene. 2016;35:1334-40 pubmed publisher
	Palla A, Piazzolla D, Alcazar N, CaÃ±amero M, GraÃ±a O, GÃ³mez LÃ³pez G, et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015;5:10205 pubmed publisher
	Bertacchi M, Pandolfini L, D Onofrio M, Brandi R, Cremisi F. The double inhibition of endogenously produced BMP and Wnt factors synergistically triggers dorsal telencephalic differentiation of mouse ES cells. Dev Neurobiol. 2015;75:66-79 pubmed publisher
	Carelli S, Messaggio F, Canazza A, Hebda D, Caremoli F, Latorre E, et al. Characteristics and Properties of Mesenchymal Stem Cells Derived From Microfragmented Adipose Tissue. Cell Transplant. 2015;24:1233-52 pubmed publisher
	Zimov S, Yazulla S. Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells. Cell Tissue Res. 2008;333:1-16 pubmed publisher
	Quraishi S, Gayet J, Morgans C, Duvoisin R. Distribution of group-III metabotropic glutamate receptors in the retina. J Comp Neurol. 2007;501:931-43 pubmed

product information

brand :

Novus

master code :

NB600-201

SKU :

NB600-201

product name :

PKC alpha Antibody (MC5)

description :

The PKC alpha Antibody (MC5) from Novus Biologicals is a mouse monoclonal antibody to PKC alpha. This antibody reacts with human, mouse, rat, porcine, bovine, canine, chicken, fish, baboon, monkey, rabbit, sheep, zebrafish. The PKC alpha Antibody (MC5) has been validated for the following applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Radioimmunoassay.

target :

PKC alpha

category :

Primary Antibodies

sizes available :

0.1 ml (also 0.025 ml)

buffer :

Tris-Glycine and 0.15 M NaCl

clonality :

Monoclonal

clone :

MC5

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Purified bovine brain Protein Kinase C

isotype :

IgG2a

purity :

Protein A purified

species :

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Fish, Baboon, Monkey, Rabbit, Sheep, Zebrafish

specificity :

This antibody is capable of detecting PKC beta as well

gene symbol :

PRKCA

accessionNumbers :

P17252, P04409

applications :

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Radioimmunoassay

USD :

356

USD 2023 :

379 USD

alt names :

AAG6, aging-associated gene 6, EC 2.7.11, EC 2.7.11.13, MGC129901, PKC-A, PKC-alpha, PKCAMGC129900, PRKACA, protein kinase C alpha type, protein kinase C, alpha

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage