product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
CD31 / PECAM-1 Antibody (MEC13.3)
catalog :
NB600-1475
quantity :
0.1 ml (also 0.025 ml)
price :
299 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MEC13.3
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Novus Biologicals product webpage
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:500; loading ...; fig s1a
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1c
Niu F, Liao K, Hu G, Sil S, Callen S, Guo M, et al. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS. J Cell Biol. 2019;218:700-721 pubmed publisher
  • immunocytochemistry; mouse; fig 1
Villaseñor R, Ozmen L, Messaddeq N, Grüninger F, Loetscher H, Keller A, et al. Trafficking of Endogenous Immunoglobulins by Endothelial Cells at the Blood-Brain Barrier. Sci Rep. 2016;6:25658 pubmed publisher
  • immunocytochemistry; mouse
Takeuchi K, Yanai R, Kumase F, Morizane Y, Suzuki J, Kayama M, et al. EGF-like-domain-7 is required for VEGF-induced Akt/ERK activation and vascular tube formation in an ex vivo angiogenesis assay. PLoS ONE. 2014;9:e91849 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Voswinckel R, Ziegelhoeffer T, Heil M, Kostin S, Breier G, Mehling T, et al. Circulating vascular progenitor cells do not contribute to compensatory lung growth. Circ Res. 2003;93:372-9 pubmed
  • flow cytometry; mouse
Baldwin H, Shen H, Yan H, DeLisser H, Chung A, Mickanin C, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994;120:2539-53 pubmed
Li W, Zhan M, Quan Y, Wang H, Hua S, Li Y, et al. Modulating the tumor immune microenvironment with sunitinib malate supports the rationale for combined treatment with immunotherapy. Int Immunopharmacol. 2020;81:106227 pubmed publisher
Liu F, Liu Q, Yuan F, Guo S, Liu J, Sun Z, et al. Erg mediates downregulation of claudin-5 in the brain endothelium of a murine experimental model of cerebral malaria. FEBS Lett. 2019;593:2585-2595 pubmed publisher
Abraham V, Cao G, Parambath A, Lawal F, Handumrongkul C, DEBS R, et al. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination. Int J Oncol. 2018;53:488-502 pubmed publisher
Perales Puchalt A, Perez Sanz J, Payne K, Svoronos N, Allegrezza M, Chaurio R, et al. Frontline Science: Microbiota reconstitution restores intestinal integrity after cisplatin therapy. J Leukoc Biol. 2018;103:799-805 pubmed publisher
Hernandez R, England C, Yang Y, Valdovinos H, Liu B, Wong H, et al. ImmunoPET imaging of tissue factor expression in pancreatic cancer with 89Zr-Df-ALT-836. J Control Release. 2017;264:160-168 pubmed publisher
Demars K, Pacheco S, Yang C, Siwarski D, Candelario Jalil E. Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice. Front Neurol. 2017;8:363 pubmed publisher
Takeda A, Hossain M, Rantakari P, Simmons S, Sasaki N, Salmi M, et al. Thymocytes in Lyve1-CRE/S1pr1f/f Mice Accumulate in the Thymus due to Cell-Intrinsic Loss of Sphingosine-1-Phosphate Receptor Expression. Front Immunol. 2016;7:489 pubmed
Salatino M, Croci D, Laderach D, Compagno D, Gentilini L, Dalotto Moreno T, et al. Regulation of galectins by hypoxia and their relevance in angiogenesis: strategies and methods. Methods Mol Biol. 2015;1207:293-304 pubmed publisher
DeLisser H, Christofidou Solomidou M, Strieter R, Burdick M, Robinson C, Wexler R, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997;151:671-7 pubmed
Christofidou Solomidou M, Nakada M, Williams J, Muller W, DeLisser H. Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation. J Immunol. 1997;158:4872-8 pubmed
Vecchi A, Garlanda C, Lampugnani M, Resnati M, Matteucci C, Stoppacciaro A, et al. Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol. 1994;63:247-54 pubmed
Rosenblum W, Murata S, Nelson G, Werner P, Ranken R, Harmon R. Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles. Am J Pathol. 1994;145:33-6 pubmed
image
image 1 :
Novus Biologicals NB600-1475 image 1
Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with CD31/PECAM-1 Antibody (MEC13.3) at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
product information
brand :
Novus
master code :
NB600-1475
SKU :
NB600-1475
product name :
CD31 / PECAM-1 Antibody (MEC13.3)
description :
The CD31/PECAM-1 Antibody (MEC13.3) from Novus is a rat monoclonal antibody to CD31/PECAM-1. This antibody reacts with human, mouse. The CD31/PECAM-1 Antibody (MEC13.3) has been validated for the following applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, In vitro assay, Flow (Cell Surface), CyTOF-ready.
target :
CD31 / PECAM-1
category :
Primary Antibodies
unit size :
0.1 ml (also 0.025 ml)
buffer :
PBS
clonality :
Monoclonal
clone :
MEC13.3
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Rat
immunogen :
This CD31 / PECAM-1 Antibody (MEC13.3) was developed against mouse endothelial cell line T-end.
isotype :
IgG2a Kappa
purity :
Protein G purified
species :
Human, Mouse
theoretical molecular weight :
82.5 kDa
gene symbol :
PECAM1
catalog number base :
NB600-1475
applications :
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, In vitro assay, Flow (Cell Surface), CyTOF-ready
USD :
299 USD
alt names :
adhesion molecule, CD31, CD31 antigen, CD31/EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA', MEC 13.3 CD31, MEC 13.3 Clone, PECA1, PECAM-1, PECAM-1, CD31/EndoCAM, platelet endothelial cell adhesion molecule, platelet endothelial cell adhesion molecule-1, platelet/endothelial cell adhesion molecule
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.