product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
catalog :
NB600-1159
quantity :
0.1 mg (also 0.025 mg)
price :
459 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF700
clone name :
114C307.1
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 75
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig st2
Tiffen J, Gunatilake D, Gallagher S, Gowrishankar K, Heinemann A, Cullinane C, et al. Targeting activating mutations of EZH2 leads to potent cell growth inhibition in human melanoma by derepression of tumor suppressor genes. Oncotarget. 2015;6:27023-36 pubmed publisher
  • western blot; human; fig 4
Heinemann A, Cullinane C, De Paoli Iseppi R, Wilmott J, Gunatilake D, Madore J, et al. Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling. Oncotarget. 2015;6:21507-21 pubmed
  • western blot; human
Wang Y, Lv J, Cheng Y, DU J, Chen D, Li C, et al. Apoptosis induced by Ginkgo biloba (EGb761) in melanoma cells is Mcl-1-dependent. PLoS ONE. 2015;10:e0124812 pubmed publisher
  • western blot; human
Sigurðsson H, Olesen C, Dybboe R, Lauritzen G, Pedersen S. Constitutively active ErbB2 regulates cisplatin-induced cell death in breast cancer cells via pro- and antiapoptotic mechanisms. Mol Cancer Res. 2015;13:63-77 pubmed publisher
  • western blot; human
Pang X, Zhang J, Lopez H, Wang Y, Li W, O Neill K, et al. The carboxyl-terminal tail of Noxa protein regulates the stability of Noxa and Mcl-1. J Biol Chem. 2014;289:17802-11 pubmed publisher
  • western blot; human
Thorburn J, Andrysik Z, Staskiewicz L, Gump J, Maycotte P, Oberst A, et al. Autophagy controls the kinetics and extent of mitochondrial apoptosis by regulating PUMA levels. Cell Rep. 2014;7:45-52 pubmed publisher
  • western blot; human
Rao F, Cha J, Xu J, Xu R, Vandiver M, Tyagi R, et al. Inositol pyrophosphates mediate the DNA-PK/ATM-p53 cell death pathway by regulating CK2 phosphorylation of Tti1/Tel2. Mol Cell. 2014;54:119-132 pubmed publisher
  • western blot; human
Godbersen J, Humphries L, Danilova O, Kebbekus P, Brown J, Eastman A, et al. The Nedd8-activating enzyme inhibitor MLN4924 thwarts microenvironment-driven NF-?B activation and induces apoptosis in chronic lymphocytic leukemia B cells. Clin Cancer Res. 2014;20:1576-89 pubmed publisher
  • western blot; human
Wroblewski D, Jiang C, Croft A, Farrelly M, Zhang X, Hersey P. OBATOCLAX and ABT-737 induce ER stress responses in human melanoma cells that limit induction of apoptosis. PLoS ONE. 2013;8:e84073 pubmed publisher
  • western blot; human
Parrondo R, de las Pozas A, Reiner T, Perez Stable C. ABT-737, a small molecule Bcl-2/Bcl-xL antagonist, increases antimitotic-mediated apoptosis in human prostate cancer cells. Peerj. 2013;1:e144 pubmed publisher
  • western blot; human
Dabir S, Kluge A, McColl K, Liu Y, Lam M, Halmos B, et al. PIAS3 activates the intrinsic apoptotic pathway in non-small cell lung cancer cells independent of p53 status. Int J Cancer. 2014;134:1045-54 pubmed publisher
  • western blot; mouse
Thomas R, Roberts D, Kubli D, Lee Y, Quinsay M, Owens J, et al. Loss of MCL-1 leads to impaired autophagy and rapid development of heart failure. Genes Dev. 2013;27:1365-77 pubmed publisher
  • western blot; mouse
Ryder C, McColl K, Zhong F, Distelhorst C. Acidosis promotes Bcl-2 family-mediated evasion of apoptosis: involvement of acid-sensing G protein-coupled receptor Gpr65 signaling to Mek/Erk. J Biol Chem. 2012;287:27863-75 pubmed publisher
  • immunocytochemistry; human
Wong C, Anderson D, Lee E, Fairlie W, Ludlam M. Direct visualization of Bcl-2 family protein interactions using live cell fluorescent protein redistribution assays. Cell Death Dis. 2012;3:e288 pubmed publisher
  • western blot; mouse
Chen M, Felix K, Wang J. Immune regulation through mitochondrion-dependent dendritic cell death induced by T regulatory cells. J Immunol. 2011;187:5684-92 pubmed publisher
  • western blot; human
Kuo K, Chen Y, Chen L, Li C, Lan Y, Chang F, et al. Involvement of phorbol-12-myristate-13-acetate-induced protein 1 in goniothalamin-induced TP53-dependent and -independent apoptosis in hepatocellular carcinoma-derived cells. Toxicol Appl Pharmacol. 2011;256:8-23 pubmed publisher
  • western blot; human
Bodet L, Menoret E, Descamps G, Pellat Deceunynck C, Bataille R, Le Gouill S, et al. BH3-only protein Bik is involved in both apoptosis induction and sensitivity to oxidative stress in multiple myeloma. Br J Cancer. 2010;103:1808-14 pubmed publisher
  • western blot; human
Vilgelm A, Washington M, Wei J, Chen H, Prassolov V, Zaika A. Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors. Mol Cancer Ther. 2010;9:693-705 pubmed publisher
  • western blot; human
Cheung M, Pei J, Pei Y, Jhanwar S, Pass H, Testa J. The promyelocytic leukemia zinc-finger gene, PLZF, is frequently downregulated in malignant mesothelioma cells and contributes to cell survival. Oncogene. 2010;29:1633-40 pubmed publisher
  • western blot; human
Amschler K, Schön M, Pletz N, Wallbrecht K, Erpenbeck L, Schön M. NF-kappaB inhibition through proteasome inhibition or IKKbeta blockade increases the susceptibility of melanoma cells to cytostatic treatment through distinct pathways. J Invest Dermatol. 2010;130:1073-86 pubmed publisher
  • western blot; human
Stankiewicz A, Livingstone A, Mohseni N, Mosser D. Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Cell Death Differ. 2009;16:638-47 pubmed publisher
  • western blot; human
Lee W, Belkhiri A, Lockhart A, Merchant N, Glaeser H, Harris E, et al. Overexpression of OATP1B3 confers apoptotic resistance in colon cancer. Cancer Res. 2008;68:10315-23 pubmed publisher
  • western blot; human
Hallaert D, Jaspers A, van Noesel C, van Oers M, Kater A, Eldering E. c-Abl kinase inhibitors overcome CD40-mediated drug resistance in CLL: implications for therapeutic targeting of chemoresistant niches. Blood. 2008;112:5141-9 pubmed publisher
  • western blot; human
Zhang L, Hao Y, Hu C, Ye Y, Xie Q, Thorne R, et al. Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells. Anticancer Drugs. 2008;19:159-66 pubmed publisher
  • western blot; human
Erster S, Mihara M, Kim R, Petrenko O, Moll U. In vivo mitochondrial p53 translocation triggers a rapid first wave of cell death in response to DNA damage that can precede p53 target gene activation. Mol Cell Biol. 2004;24:6728-41 pubmed
  • immunocytochemistry; human
  • western blot; human
Yakovlev A, Di Giovanni S, Wang G, Liu W, Stoica B, Faden A. BOK and NOXA are essential mediators of p53-dependent apoptosis. J Biol Chem. 2004;279:28367-74 pubmed
Al Shboul S, El Sadoni M, Alhesa A, Abu Shahin N, Abuquteish D, Abu Al Karsaneh O, et al. NOXA expression is downregulated in human breast cancer undergoing incomplete pathological response and senescence after neoadjuvant chemotherapy. Sci Rep. 2023;13:15903 pubmed publisher
Huang A, Chin H, Reljic B, Djajawi T, Tan I, Gong J, et al. Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins. Cell Death Differ. 2022;: pubmed publisher
Asuzu D, Alvarez R, Fletcher P, Mandal D, Johnson K, Wu W, et al. Pituitary adenomas evade apoptosis via noxa deregulation in Cushing's disease. Cell Rep. 2022;40:111223 pubmed publisher
Liu H, Zhang Z, Zhen P, Zhou M. High Expression of VSTM2L Induced Resistance to Chemoradiotherapy in Rectal Cancer through Downstream IL-4 Signaling. J Immunol Res. 2021;2021:6657012 pubmed publisher
Zheng J, Zhuo L, Ran D, Ma Y, Luo T, Zhao H, et al. Cadmium induces apoptosis via generating reactive oxygen species to activate mitochondrial p53 pathway in primary rat osteoblasts. Toxicology. 2020;446:152611 pubmed publisher
Tseng H, Dreyer J, Emran A, Gunatilake D, Pirozyan M, Cullinane C, et al. Co-targeting bromodomain and extra-terminal proteins and MCL1 induces synergistic cell death in melanoma. Int J Cancer. 2020;: pubmed publisher
Djajawi T, Liu L, Gong J, Huang A, Luo M, Xu Z, et al. MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex. Cell Death Differ. 2020;: pubmed publisher
Pilling A, Hwang C. Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide. Prostate. 2019;79:1347-1359 pubmed publisher
Gonzalez P, O Prey J, Cardaci S, Barthet V, Sakamaki J, Beaumatin F, et al. Mannose impairs tumour growth and enhances chemotherapy. Nature. 2018;563:719-723 pubmed publisher
Minieri V, De Dominici M, Porazzi P, Mariani S, Spinelli O, Rambaldi A, et al. Targeting STAT5 or STAT5-Regulated Pathways Suppresses Leukemogenesis of Ph+ Acute Lymphoblastic Leukemia. Cancer Res. 2018;78:5793-5807 pubmed publisher
Gallagher S, Gunatilake D, Beaumont K, Sharp D, Tiffen J, Heinemann A, et al. HDAC inhibitors restore BRAF-inhibitor sensitivity by altering PI3K and survival signalling in a subset of melanoma. Int J Cancer. 2018;142:1926-1937 pubmed publisher
Antignani A, Segal D, Simon N, Kreitman R, Huang D, Fitzgerald D. Essential role for Bim in mediating the apoptotic and antitumor activities of immunotoxins. Oncogene. 2017;36:4953-4962 pubmed publisher
Zhang J, Huang K, O Neill K, Pang X, Luo X. Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Cell Death Dis. 2016;7:e2266 pubmed publisher
Wei J, Noto J, Zaika E, Romero Gallo J, Piazuelo M, Schneider B, et al. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut. 2015;64:1040-8 pubmed publisher
Pascutti M, Jak M, Tromp J, Derks I, Remmerswaal E, Thijssen R, et al. IL-21 and CD40L signals from autologous T cells can induce antigen-independent proliferation of CLL cells. Blood. 2013;122:3010-9 pubmed publisher
Adamski J, Price A, Dive C, Makin G. Hypoxia-induced cytotoxic drug resistance in osteosarcoma is independent of HIF-1Alpha. PLoS ONE. 2013;8:e65304 pubmed publisher
Zinn R, Gardner E, Dobromilskaya I, Murphy S, Marchionni L, Hann C, et al. Combination treatment with ABT-737 and chloroquine in preclinical models of small cell lung cancer. Mol Cancer. 2013;12:16 pubmed publisher
Tromp J, Geest C, Breij E, Elias J, van Laar J, Luijks D, et al. Tipping the Noxa/Mcl-1 balance overcomes ABT-737 resistance in chronic lymphocytic leukemia. Clin Cancer Res. 2012;18:487-98 pubmed publisher
Chu R, Terrano D, Chambers T. Cdk1/cyclin B plays a key role in mitotic arrest-induced apoptosis by phosphorylation of Mcl-1, promoting its degradation and freeing Bak from sequestration. Biochem Pharmacol. 2012;83:199-206 pubmed publisher
Ishikita H. Proton-binding sites of acid-sensing ion channel 1. PLoS ONE. 2011;6:e16920 pubmed publisher
Chen C, Huang P, Chu P, Chen M, Chou C, Wang D, et al. Energy restriction-mimetic agents induce apoptosis in prostate cancer cells in part through epigenetic activation of KLF6 tumor suppressor gene expression. J Biol Chem. 2011;286:9968-76 pubmed publisher
Poulsen R, Carr A, Hulley P. Protection against glucocorticoid-induced damage in human tenocytes by modulation of ERK, Akt, and forkhead signaling. Endocrinology. 2011;152:503-14 pubmed publisher
Zhang L, Lopez H, George N, Liu X, Pang X, Luo X. Selective involvement of BH3-only proteins and differential targets of Noxa in diverse apoptotic pathways. Cell Death Differ. 2011;18:864-73 pubmed publisher
Fahham N, Sardari S, Ostad S, Vaziri B, Ghahremani M. C-terminal domain of p16(INK4a) is adequate in inducing cell cycle arrest, growth inhibition and CDK4/6 interaction similar to the full length protein in HT-1080 fibrosarcoma cells. J Cell Biochem. 2010;111:1598-606 pubmed publisher
Tonino S, van Laar J, Van Oers M, Wang J, Eldering E, Kater A. ROS-mediated upregulation of Noxa overcomes chemoresistance in chronic lymphocytic leukemia. Oncogene. 2011;30:701-13 pubmed publisher
Hadji A, Clybouw C, Auffredou M, Alexia C, Poalas K, Burlion A, et al. Caspase-3 triggers a TPCK-sensitive protease pathway leading to degradation of the BH3-only protein puma. Apoptosis. 2010;15:1529-39 pubmed publisher
Zall H, Weber A, Besch R, Zantl N, Hacker G. Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1. Mol Cancer. 2010;9:164 pubmed publisher
Sosa Seda I, Mott J, Akazawa Y, Barreyro F, Bronk S, Kaufmann S, et al. Noxa mediates hepatic stellate cell apoptosis by proteasome inhibition. Hepatol Res. 2010;40:701-10 pubmed publisher
de Bruijn M, Raats D, Hoogwater F, van Houdt W, Cameron K, Medema J, et al. Oncogenic KRAS sensitises colorectal tumour cells to chemotherapy by p53-dependent induction of Noxa. Br J Cancer. 2010;102:1254-64 pubmed publisher
Lopez H, Zhang L, George N, Liu X, Pang X, Evans J, et al. Perturbation of the Bcl-2 network and an induced Noxa/Bcl-xL interaction trigger mitochondrial dysfunction after DNA damage. J Biol Chem. 2010;285:15016-26 pubmed publisher
Streetly M, Maharaj L, Joel S, Schey S, Gribben J, Cotter F. GCS-100, a novel galectin-3 antagonist, modulates MCL-1, NOXA, and cell cycle to induce myeloma cell death. Blood. 2010;115:3939-48 pubmed publisher
Tahir S, Wass J, Joseph M, Devanarayan V, Hessler P, Zhang H, et al. Identification of expression signatures predictive of sensitivity to the Bcl-2 family member inhibitor ABT-263 in small cell lung carcinoma and leukemia/lymphoma cell lines. Mol Cancer Ther. 2010;9:545-57 pubmed publisher
Ackler S, Mitten M, Foster K, Oleksijew A, Refici M, Tahir S, et al. The Bcl-2 inhibitor ABT-263 enhances the response of multiple chemotherapeutic regimens in hematologic tumors in vivo. Cancer Chemother Pharmacol. 2010;66:869-80 pubmed publisher
Paoluzzi L, Scotto L, Marchi E, Zain J, Seshan V, O Connor O. Romidepsin and belinostat synergize the antineoplastic effect of bortezomib in mantle cell lymphoma. Clin Cancer Res. 2010;16:554-65 pubmed publisher
High L, Szymanska B, Wilczynska Kalak U, Barber N, O Brien R, Khaw S, et al. The Bcl-2 homology domain 3 mimetic ABT-737 targets the apoptotic machinery in acute lymphoblastic leukemia resulting in synergistic in vitro and in vivo interactions with established drugs. Mol Pharmacol. 2010;77:483-94 pubmed publisher
Burgos J, King Lyons N, Connell T. Expression of BfrH, a putative siderophore receptor of Bordetella bronchiseptica, is regulated by iron, Fur1, and the extracellular function sigma factor EcfI. Infect Immun. 2010;78:1147-62 pubmed publisher
van Gent R, Kater A, Otto S, Jaspers A, Borghans J, Vrisekoop N, et al. In vivo dynamics of stable chronic lymphocytic leukemia inversely correlate with somatic hypermutation levels and suggest no major leukemic turnover in bone marrow. Cancer Res. 2008;68:10137-44 pubmed publisher
Jiang C, Lucas K, Avery Kiejda K, Wade M, deBock C, Thorne R, et al. Up-regulation of Mcl-1 is critical for survival of human melanoma cells upon endoplasmic reticulum stress. Cancer Res. 2008;68:6708-17 pubmed publisher
Condorelli F, Gnemmi I, Vallario A, Genazzani A, Canonico P. Inhibitors of histone deacetylase (HDAC) restore the p53 pathway in neuroblastoma cells. Br J Pharmacol. 2008;153:657-68 pubmed
Tahir S, Yang X, Anderson M, Morgan Lappe S, Sarthy A, Chen J, et al. Influence of Bcl-2 family members on the cellular response of small-cell lung cancer cell lines to ABT-737. Cancer Res. 2007;67:1176-83 pubmed
Yu F, Watts R, Zhang X, Borrow J, Hersey P. Involvement of BH3-only proapoptotic proteins in mitochondrial-dependent Phenoxodiol-induced apoptosis of human melanoma cells. Anticancer Drugs. 2006;17:1151-61 pubmed
Smit L, Hallaert D, Spijker R, de Goeij B, Jaspers A, Kater A, et al. Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity. Blood. 2007;109:1660-8 pubmed
Yoshida A, Takemura H, Inoue H, Miyashita T, Ueda T. Inhibition of glutathione synthesis overcomes Bcl-2-mediated topoisomerase inhibitor resistance and induces nonapoptotic cell death via mitochondrial-independent pathway. Cancer Res. 2006;66:5772-80 pubmed
Xie W, Jiang P, Miao L, Zhao Y, Zhimin Z, Qing L, et al. Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1. Nucleic Acids Res. 2006;34:2046-55 pubmed
Simbulan Rosenthal C, Daher A, Trabosh V, Chen W, Gerstel D, Soeda E, et al. Id3 induces a caspase-3- and -9-dependent apoptosis and mediates UVB sensitization of HPV16 E6/7 immortalized human keratinocytes. Oncogene. 2006;25:3649-60 pubmed
Chen W, Wang D, Yen R, Luo J, Gu W, Baylin S. Tumor suppressor HIC1 directly regulates SIRT1 to modulate p53-dependent DNA-damage responses. Cell. 2005;123:437-48 pubmed
Kojima K, Konopleva M, Samudio I, Shikami M, Cabreira Hansen M, McQueen T, et al. MDM2 antagonists induce p53-dependent apoptosis in AML: implications for leukemia therapy. Blood. 2005;106:3150-9 pubmed
Mihara M, Erster S, Zaika A, Petrenko O, Chittenden T, Pancoska P, et al. p53 has a direct apoptogenic role at the mitochondria. Mol Cell. 2003;11:577-90 pubmed
Deverman B, Cook B, Manson S, Niederhoff R, Langer E, Rosová I, et al. Bcl-xL deamidation is a critical switch in the regulation of the response to DNA damage. Cell. 2002;111:51-62 pubmed
product information
brand :
Novus Biologicals, a Bio-Techne brand
catalog number base :
NB600-1159
SKU :
NB600-1159
product name :
Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
description :
The Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal from Novus Biologicals is a mouse monoclonal antibody to Noxa. This antibody reacts with human,mouse,rat. The Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal has been validated for the following applications: Flow Cytometry,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence.
target :
Noxa
unit size :
0.1 mg (also 0.025 mg)
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
114C307.1
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
This Noxa antibody was developed by immunizing mice with a fusion protein containing human Noxa.
isotype :
IgG1 Kappa
purity :
Protein G purified
species :
Human,Mouse,Rat
specificity :
An ~80 kD background band may also be seen in Jurkat cell lysate
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
theoretical molecular weight :
14.96 kDa
gene symbol :
PMAIP1
accessionNumbers :
Q13794
applications :
Flow Cytometry,IF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence
USD :
449
USD 2025 :
459 USD
alt names :
adult T cell leukemia-derived PMA-responsive, APR, Immediate-early-response protein APR, NOXAPMA-induced protein 1, phorbol-12-myristate-13-acetate-induced protein 1, PMA-Induced Protein 1, Protein Noxa
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.