product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Mouse Monoclonal CD34 Antibody
catalog :
NB500-608R
quantity :
0.05 mg
price :
429.00 USD
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
neutralization, flow cytometry
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB500-608R
Product Name :
Mouse Monoclonal CD34 Antibody
List Price :
429.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 mg
AltNames :
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Background :
The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
4H11[APG]
Concentration :
1.0 mg/ml
Conjugate :
DyLight 550
Application Summary :
Blocking/Neutralizing, Flow Cytometry
Host :
Mouse
Immunogen :
Tissue / cell preparation (Human). Permanent human cell line derived from peripheral leukocytes of a patient suffering from chronic myeloid leukaemia.
Isotype :
IgG1
Localization :
Membrane; Single-pass type I membrane protein.
Marker :
Hematopoietic Stem Cell Marker
Preservative :
No Preservative
Purity :
Affinity purified
CrossReactivity :
Reacts with Human.
Specificity :
This reacts with Class III epitope on CD34 antigen, a monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Storage :
Store at 4C in the dark.
Swissprot Human :
P28906
TMW :
40 kDa
Uses :
This product has been validated for use in Flow Cytometry, Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry. Western blot sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Recommended positive control for IHC-P: Placenta endothelium.
Research Area :
Cell Biology, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Hypoxia, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.