product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Mouse Monoclonal CD45 Antibody
catalog :
NB500-319G
quantity :
0.05 mg
price :
429.00 USD
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
western blot, immunoprecipitation, immunohistochemistry, flow cytometry
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB500-319G
Product Name :
Mouse Monoclonal CD45 Antibody
List Price :
429.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 mg
AltNames :
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200receptor type, c polypeptide, T200 leukocyte common antigen, T200 glycoprotein
Background :
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.
Buffer :
50mM sodium borate
Clonality :
Monoclonal
Clone :
MEM-28
Concentration :
1.0 mg/ml
Conjugate :
DyLight 488
Application Summary :
Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation
Host :
Mouse
Immunogen :
Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).
Isotype :
IgG1
Localization :
Type I membrane protein
Preservative :
No Preservative
Purity :
Protein A purified
CrossReactivity :
Cross-reacts with Human.
Specificity :
This reacts with all alternative forms of CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type one transmembrane protein expressed at high level on all cells of hematopoetic origin, except erythrocytes and platelets.
Storage :
Store at 4C in the dark.
Uses :
Flow Cytometry Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Antigen retrieval is not essential but may optimise staining. IP: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Research Area :
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.