product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rat Monoclonal Bromodeoxyuridine/BrdU Antibody
catalog :
NB500-169R
quantity :
0.1 mg
price :
319.00 USD
clonality :
monoclonal
host :
rat
conjugate :
DyLight 550
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB500-169R
Product Name :
Rat Monoclonal Bromodeoxyuridine/BrdU Antibody
List Price :
319.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 mg
AltNames :
Bromodeoxyuridine, BUdr
Background :
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Buffer :
50mM Sodium Borate
Clonality :
Monoclonal
Clone :
BU1/75 (ICR1)
Conjugate :
DyLight 550
Application Summary :
Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Host :
Rat
Immunogen :
Made to Chemical BrdU
Isotype :
IgG2a
Localization :
Nuclear
Marker :
Proliferation Marker
Preservative :
No Preservative
Purity :
Protein G purified
Specificity :
This reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts weakly with chlorodeoxyuridine, but does not cross react with thymidine or iododeoxyuridine.
Storage :
Store at 4C in the dark.
Uses :
Clone BU1/75 (ICR1) can be used for labeling paraffin-embedded tissue sections fixed in formalin. Denaturation of the DNA is critical for successful staining of BrdU. This can be achieved by exposing cells to heat, or acid. For heat-induced epitope retrieval, 10mM citrate buffer pH6.0 is recommended. Alternatively, a 30 min incubation in 2M HCl can be performed. The HCl must then be neutralized for 2 min with 0.1 M Na2B4O7. Pretreatment of tissues with proteinase K should be avoided. Immunohistochemistry-Frozen was reported in scientific literature.
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.