product summary
Loading...
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
PCNA Antibody (PC10)
catalog :
NB500-106
quantity :
0.1 ml (also 0.025 ml)
price :
449 USD
clonality :
monoclonal
host :
mouse
conjugate :
DyLight 350
clone name :
PC10
reactivity :
primate, fishes , human, mouse, rat, chicken, zebrafish , brewer's yeast
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; rat; 1:250; loading ...; tbl 1
  • western blot; rat; 1:1000; loading ...; tbl 1
Stock P, Bielohuby M, Staege M, Hsu M, Bidlingmaier M, Christ B. Impairment of Host Liver Repopulation by Transplanted Hepatocytes in Aged Rats and the Release by Short-Term Growth Hormone Treatment. Am J Pathol. 2017;187:553-569 pubmed publisher
  • chromatin immunoprecipitation; brewer's yeast; fig 1
Ivanova I, Maringele L. Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt. Nucleic Acids Res. 2016;44:3728-38 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Houlihan S, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. elife. 2014;3:e03297 pubmed publisher
  • immunohistochemistry - paraffin section; rat
Zhu J, Zhu C, Ge S, Zhang M, Jiang L, Cui J, et al. Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model. J Appl Microbiol. 2014;117:208-16 pubmed publisher
  • immunohistochemistry - paraffin section; rat
Zhang M, Wang F, Jiang L, Liu R, Zhang L, LEI X, et al. Lactobacillus salivarius REN inhibits rat oral cancer induced by 4-nitroquioline 1-oxide. Cancer Prev Res (Phila). 2013;6:686-94 pubmed publisher
  • immunohistochemistry - paraffin section; rat
Sheu M, Lin H, Yang Y, Chou C, Chien Y, Wu T, et al. Demethoxycurcumin, a major active curcuminoid from Curcuma longa, suppresses balloon injury induced vascular smooth muscle cell migration and neointima formation: an in vitro and in vivo study. Mol Nutr Food Res. 2013;57:1586-97 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Errami Y, Brim H, Oumouna Benachour K, Oumouna M, Naura A, Kim H, et al. ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability. PLoS ONE. 2013;8:e57871 pubmed publisher
  • immunohistochemistry; mouse
Chien Y, Huang G, Cheng H, Wu C, Sheu M. Hispolon attenuates balloon-injured neointimal formation and modulates vascular smooth muscle cell migration via AKT and ERK phosphorylation. J Nat Prod. 2012;75:1524-33 pubmed
  • western blot; human
Montaldi A, Sakamoto Hojo E. Methoxyamine sensitizes the resistant glioblastoma T98G cell line to the alkylating agent temozolomide. Clin Exp Med. 2013;13:279-88 pubmed publisher
  • immunohistochemistry; human
Hasenstein J, Kasmerchak K, Buehler D, Hafez G, Cleary K, Moody J, et al. Efficacy of Tie2 receptor antagonism in angiosarcoma. Neoplasia. 2012;14:131-40 pubmed
  • immunohistochemistry - paraffin section; mouse
  • immunocytochemistry; mouse
Kuo W, Yu M, Lee J, Tsai C, Chen T, Chen M. Imatinib mesylate improves liver regeneration and attenuates liver fibrogenesis in CCL4-treated mice. J Gastrointest Surg. 2012;16:361-9 pubmed publisher
  • immunohistochemistry - paraffin section; fishes
Ishikawa Y, Yamamoto N, Yasuda T, Yoshimoto M, Ito H. Morphogenesis of the medaka cerebellum, with special reference to the mesencephalic sheet, a structure homologous to the rostrolateral part of mammalian anterior medullary velum. Brain Behav Evol. 2010;75:88-103 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • flow cytometry; human
Woods A, Hall P, Shepherd N, Hanby A, Waseem N, Lane D, et al. The assessment of proliferating cell nuclear antigen (PCNA)immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis. Histopathology. 2002;41:165-71 pubmed
  • immunohistochemistry - paraffin section; human
  • immunoprecipitation; human
  • immunocytochemistry; human
  • ELISA; human
  • western blot; human
  • immunohistochemistry - paraffin section; primate
  • immunoprecipitation; primate
  • immunocytochemistry; primate
  • ELISA; primate
  • western blot; primate
Waseem N, Lane D. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci. 1990;96 ( Pt 1):121-9 pubmed
Rothermund K, Calabrese T, Syed Picard F. Differential Effects of Escherichia coli- Versus Porphyromonas gingivalis-derived Lipopolysaccharides on Dental Pulp Stem Cell Differentiation in Scaffold-free Engineered Tissues. J Endod. 2022;48:1378-1386.e2 pubmed publisher
Lundine D, Annor G, Chávez V, Maimos S, Syed Z, Jiang S, et al. The C-terminus of Gain-of-Function Mutant p53 R273H Is Required for Association with PARP1 and Poly-ADP-Ribose. Mol Cancer Res. 2022;20:1799-1810 pubmed publisher
Meligy F, El Deen Mohammed H, Mostafa T, Elfiky M, El Sayed Mohamed Ashry I, Abd Eldayem A, et al. Therapeutic Potential of Mesenchymal Stem Cells versus Omega n - 3 Polyunsaturated Fatty Acids on Gentamicin-Induced Cardiac Degeneration. Pharmaceutics. 2022;14: pubmed publisher
Martins R, Zamzam M, Tracey White D, Moosajee M, Thummel R, Henriques C, et al. Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina. Aging Cell. 2022;21:e13597 pubmed publisher
Chung H, Moon S, Kang S, Kim M, Lee H, Tchah H, et al. Corneal Epithelial Removal with a Newly Designed Epithelial Brush. J Ophthalmol. 2021;2021:4668056 pubmed publisher
Bender H, Fietz S, Richter F, Stanojlovic M. Alpha-Synuclein Pathology Coincides With Increased Number of Early Stage Neural Progenitors in the Adult Hippocampus. Front Cell Dev Biol. 2021;9:691560 pubmed publisher
Sakurai R, Singh H, Wang Y, Harb A, Gornes C, Liu J, et al. Effect of Perinatal Vitamin D Deficiency on Lung Mesenchymal Stem Cell Differentiation and Injury Repair Potential. Am J Respir Cell Mol Biol. 2021;65:521-531 pubmed publisher
Rossi G, Placidi M, Castellini C, Rea F, D Andrea S, Alonso G, et al. Crocetin Mitigates Irradiation Injury in an In Vitro Model of the Pubertal Testis: Focus on Biological Effects and Molecular Mechanisms. Molecules. 2021;26: pubmed publisher
Verboom L, Martens A, Priem D, Hoste E, Sze M, Vikkula H, et al. OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis. Cell Rep. 2020;30:2237-2247.e6 pubmed publisher
Wong M, Uchida M, Tsukada T. Histological differentiation of mucus cell subtypes suggests functional compartmentation in the eel esophagus. Cell Tissue Res. 2020;380:499-512 pubmed publisher
Yazdankhah M, Shang P, Ghosh S, Bhutto I, Stepicheva N, Grebe R, et al. Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease. Autophagy. 2020;16:1130-1142 pubmed publisher
Di Emidio G, Santini S, D Alessandro A, Vetuschi A, Sferra R, Artini P, et al. SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary. Biochim Biophys Acta Mol Basis Dis. 2019;1865:1389-1401 pubmed publisher
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018;15:909-912 pubmed publisher
Fukasawa H, Hashimoto H, Hiramoto K, Sugiyama M, Taniguchi K, Yoshioka K. Morphological characterization of small hepatocytes after bile duct ligation in chicken. Poult Sci. 2019;98:717-721 pubmed publisher
Yasuda T, Ishikawa Y, Shioya N, Itoh K, Kamahori M, Nagata K, et al. Radical change of apoptotic strategy following irradiation during later period of embryogenesis in medaka (Oryzias latipes). PLoS ONE. 2018;13:e0201790 pubmed publisher
Römer S, Bender H, Knabe W, Zimmermann E, Rubsamen R, Seeger J, et al. Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew (Tupaia belangeri) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents. Front Neuroanat. 2018;12:29 pubmed publisher
Sauerland C, Menzies B, Glatzle M, Seeger J, Renfree M, Fietz S. The Basal Radial Glia Occurs in Marsupials and Underlies the Evolution of an Expanded Neocortex in Therian Mammals. Cereb Cortex. 2018;28:145-157 pubmed publisher
Qin Y, Zhu Y, Luo F, Chen C, Chen X, Wu M. Killing two birds with one stone: dual blockade of integrin and FGF signaling through targeting syndecan-4 in postoperative capsular opacification. Cell Death Dis. 2017;8:e2920 pubmed publisher
Xiong W, Luo Y, Wu L, Liu F, Liu H, Li J, et al. Chemerin Stimulates Vascular Smooth Muscle Cell Proliferation and Carotid Neointimal Hyperplasia by Activating Mitogen-Activated Protein Kinase Signaling. PLoS ONE. 2016;11:e0165305 pubmed publisher
Pushchina E, Shukla S, Varaksin A, Obukhov D. Cell proliferation and apoptosis in optic nerve and brain integration centers of adult trout Oncorhynchus mykiss after optic nerve injury. Neural Regen Res. 2016;11:578-90 pubmed publisher
Hu Y, Cong X, Chen L, Qi J, Wu X, Zhou M, et al. Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-?B signaling pathway. Sci Rep. 2016;6:23977 pubmed publisher
Engler A, Tange C, Frank Bertoncelj M, Gay R, Gay S, Ospelt C. Regulation and function of SIRT1 in rheumatoid arthritis synovial fibroblasts. J Mol Med (Berl). 2016;94:173-82 pubmed publisher
Sawosz E, Jaworski S, Kutwin M, Hotowy A, Wierzbicki M, Grodzik M, et al. Toxicity of pristine graphene in experiments in a chicken embryo model. Int J Nanomedicine. 2014;9:3913-22 pubmed publisher
Williams S, Wilson J, Clark A, Mitson Salazar A, Tomashevski A, Ananth S, et al. Functional and physical interaction between the mismatch repair and FA-BRCA pathways. Hum Mol Genet. 2011;20:4395-410 pubmed publisher
Kawamura K, Tachibana M, Sunanaga T. Cell proliferation dynamics of somatic and germline tissues during zooidal life span in the colonial tunicate Botryllus primigenus. Dev Dyn. 2008;237:1812-25 pubmed publisher
product information
brand :
Novus Biologicals, a Bio-Techne Brand
catalog number base :
NB500-106
SKU :
NB500-106
product name :
PCNA Antibody (PC10)
Description :
The PCNA Antibody (PC10) from Novus is a mouse monoclonal antibody to PCNA. This antibody reacts with human, mouse, rat, porcine, chicken, drosophila, fish, marsupial, primate, rabbit, yeast, zebrafish. The PCNA Antibody (PC10) has been validated for the following applications: Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Chromatin Immunoprecipitation (ChIP).
target :
PCNA
category :
Primary Antibodies
unit size :
0.1 ml (also 0.025 ml)
buffer :
Tris-Glycine, 0.15M NaCl
clonality :
Monoclonal
clone :
PC10
concentration :
1 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
Protein A-rat PCNA fusion obtained from pC2T
isotype :
IgG2a Kappa
purity :
Protein A purified
species :
Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish
specificity :
Specific for PCNA p36 protein expressed at high levels in proliferating cells
theoretical molecular weight :
30 kDa
gene symbol :
PCNA
accessionNumbers :
P12004
applications :
Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Chromatin Immunoprecipitation (ChIP)
USD :
449 USD
alt names :
cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.