product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
CENPF Antibody - BSA Free
catalog :
NB500-101
quantity :
0.1 mg (also 0.025 mg)
price :
409 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Novus Biologicals product webpage
citations: 25
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:300; loading ...; fig 2f
  • western blot; human; 1:500; loading ...; fig 4g
Ciossani G, Overlack K, Petrovic A, Huis In t Veld P, Koerner C, Wohlgemuth S, et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem. 2018;293:10084-10101 pubmed publisher
Cmentowski V, Ciossani G, d Amico E, Wohlgemuth S, Owa M, DYNLACHT B, et al. RZZ-Spindly and CENP-E form an integrated platform to recruit dynein to the kinetochore corona. EMBO J. 2023;42:e114838 pubmed publisher
Cmentowski V, Ciossani G, d Amico E, Wohlgemuth S, Owa M, DYNLACHT B, et al. A mechanism that integrates microtubule motors of opposite polarity at the kinetochore corona. bioRxiv. 2023;: pubmed publisher
Li L, Zhang X, Zhang H, Agborbesong E, Zhou J, Calvet J, et al. Single-Cell and CellChat Resolution Identifies Collecting Duct Cell Subsets and Their Communications with Adjacent Cells in PKD Kidneys. Cells. 2022;12: pubmed publisher
Tanaka K, Suzuki K, Miyashita K, Wakasa K, Kawano M, Nakatsu Y, et al. Activation of recombinational repair in Ewing sarcoma cells carrying EWS-FLI1 fusion gene by chromosome translocation. Sci Rep. 2022;12:14764 pubmed publisher
Averbeck N, Barent C, Jakob B, Syzonenko T, Durante M, Taucher Scholz G. The Ubiquitin Ligase RNF138 Cooperates with CtIP to Stimulate Resection of Complex DNA Double-Strand Breaks in Human G1-Phase Cells. Cells. 2022;11: pubmed publisher
Zhao J, Wang F, Tian Q, Dong J, Chen L, Hu R. Involvement of miR-214-3p/FOXM1 Axis During the Progression of Psoriasis. Inflammation. 2022;45:267-278 pubmed publisher
Averbek S, Jakob B, Durante M, Averbeck N. O-GlcNAcylation Affects the Pathway Choice of DNA Double-Strand Break Repair. Int J Mol Sci. 2021;22: pubmed publisher
Renda F, Magidson V, Tikhonenko I, Fisher R, Miles C, Mogilner A, et al. Effects of malleable kinetochore morphology on measurements of intrakinetochore tension. Open Biol. 2020;10:200101 pubmed publisher
Luppino J, Park D, Nguyen S, Lan Y, Xu Z, Yunker R, et al. Cohesin promotes stochastic domain intermingling to ensure proper regulation of boundary-proximal genes. Nat Genet. 2020;52:840-848 pubmed publisher
Nath S, Nagaraju G. FANCJ helicase promotes DNA end resection by facilitating CtIP recruitment to DNA double-strand breaks. PLoS Genet. 2020;16:e1008701 pubmed publisher
Rodriguez Rodriguez J, Lewis C, McKinley K, Sikirzhytski V, Corona J, Maciejowski J, et al. Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion. Curr Biol. 2018;28:3422-3429.e5 pubmed publisher
Sikirzhytski V, Renda F, Tikhonenko I, Magidson V, McEwen B, Khodjakov A. Microtubules assemble near most kinetochores during early prometaphase in human cells. J Cell Biol. 2018;217:2647-2659 pubmed publisher
Chong T, Sarac A, Yao C, Liao L, Lyttle N, Boutros P, et al. Deregulation of the spindle assembly checkpoint is associated with paclitaxel resistance in ovarian cancer. J Ovarian Res. 2018;11:27 pubmed publisher
Yamauchi M, Shibata A, Suzuki K, Suzuki M, Niimi A, Kondo H, et al. Regulation of pairing between broken DNA-containing chromatin regions by Ku80, DNA-PKcs, ATM, and 53BP1. Sci Rep. 2017;7:41812 pubmed publisher
Magidson V, He J, Ault J, O Connell C, Yang N, Tikhonenko I, et al. Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells. J Cell Biol. 2016;212:307-19 pubmed publisher
Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. 2015;290:27053-66 pubmed publisher
Kanfer G, Courtheoux T, Peterka M, Meier S, Soste M, Melnik A, et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. 2015;6:8015 pubmed publisher
Magidson V, Paul R, Yang N, Ault J, O Connell C, Tikhonenko I, et al. Adaptive changes in the kinetochore architecture facilitate proper spindle assembly. Nat Cell Biol. 2015;17:1134-44 pubmed publisher
Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. 2015;26:3379-89 pubmed publisher
Kaida A, Miura M. Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells. Cancer Sci. 2015;106:1370-6 pubmed publisher
Nishikawa R, Goto Y, Kurozumi A, Matsushita R, Enokida H, Kojima S, et al. MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer. Int J Urol. 2015;22:867-77 pubmed publisher
Bee L, Nasca A, Zanolini A, Cendron F, d Adamo P, Costa R, et al. A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy. EMBO Mol Med. 2015;7:918-29 pubmed publisher
Nakajima N, Hagiwara Y, Oike T, Okayasu R, Murakami T, Nakano T, et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS ONE. 2015;10:e0122582 pubmed publisher
Lee I, Lee C, Lee J. CaMKKβ-AMPKα2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells. Cell Cycle. 2015;14:598-611 pubmed publisher
product information
master code :
NB500-101
SKU :
NB500-101
product name :
CENPF Antibody - BSA Free
unit size :
0.1 mg (also 0.025 mg)
description :
The CENPF Antibody - BSA Free from Novus is a rabbit polyclonal antibody to CENPF. This antibody reacts with bovine,equine,human,mouse. The CENPF Antibody - BSA Free has been validated for the following applications: Western Blot,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Whole-Mount,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Knockdown Validated.
target :
CENPF
category :
Primary Antibodies
buffer :
PBS
clonality :
Polyclonal
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]
isotype :
IgG
purity :
Immunogen affinity purified
species :
Bovine,Equine,Human,Mouse
gene symbol :
CENPF
Antibody validation :
Biological Validation
applications :
Western Blot,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Whole-Mount,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Knockdown Validated
USD :
409 USD
alt names :
AH antigen, cell-cycle-dependent 350K nuclear protein, CENF, CENP-F, CENP-F kinetochore protein, centromere protein F, centromere protein F (350/400kD, mitosin), centromere protein F, 350/400ka (mitosin), centromere protein F, 350/400kDa (mitosin), hcp-1, Kinetochore protein CENPF, mitosin, PRO1779
storage :
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.