product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Niemann-Pick C1 Antibody - BSA Free
catalog :
NB400-148
quantity :
0.1 ml (also 0.025 ml)
price :
399 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
AP-MAB0708
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 29
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:2500; loading ...; fig 4d
Guix F, Capitán A, Casadomé Perales Á, Palomares Perez I, López Del Castillo I, Miguel V, et al. Increased exosome secretion in neurons aging in vitro by NPC1-mediated endosomal cholesterol buildup. Life Sci Alliance. 2021;4: pubmed publisher
Fabiano M, Oikawa N, Kerksiek A, Furukawa J, Yagi H, Kato K, et al. Presenilin Deficiency Results in Cellular Cholesterol Accumulation by Impairment of Protein Glycosylation and NPC1 Function. Int J Mol Sci. 2024;25: pubmed publisher
Kim J, Park S, Kwak C, Lee Y, Song D, Jung J, et al. Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites. Cancer Commun (Lond). 2023;: pubmed publisher
N x16f skov xe1 H, Cortizo F, Schwenker L, Sachsenheimer T, Diakonov E, Tiebe M, et al. Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome. J Biol Chem. 2023;299:105088 pubmed publisher
Skop xe1 l A, Ujlaki G, Gerencs xe9 r A, Bank xf3 C, Bacs xf3 Z, Ciruela F, et al. Adenosine A2A Receptor Activation Regulates Niemann-Pick C1 Expression and Localization in Macrophages. Curr Issues Mol Biol. 2023;45:4948-4969 pubmed publisher
Martella N, Colardo M, Sergio W, Petraroia M, Varone M, Pensabene D, et al. Lavender Essential Oil Modulates Hepatic Cholesterol Metabolism in HepG2 Cells. Curr Issues Mol Biol. 2023;45:364-378 pubmed publisher
Weng Y, Shepherd D, Liu Y, Krishnan N, Robertson B, Platt N, et al. Inhibition of the Niemann-Pick C1 protein is a conserved feature of multiple strains of pathogenic mycobacteria. Nat Commun. 2022;13:5320 pubmed publisher
Colardo M, Petraroia M, Lerza L, Pensabene D, Martella N, Pallottini V, et al. NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress. Int J Mol Sci. 2022;23: pubmed publisher
Cheng Y, Zhang T, Ma X, Pratuangtham S, Zhang G, Ondrus A, et al. A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. Nat Chem Biol. 2021;17:1271-1280 pubmed publisher
Goedeke L, Canfrán Duque A, Rotllan N, Chaube B, Thompson B, Lee R, et al. MMAB promotes negative feedback control of cholesterol homeostasis. Nat Commun. 2021;12:6448 pubmed publisher
Walch P, Selkrig J, Knodler L, Rettel M, Stein F, Fernandez K, et al. Global mapping of Salmonella enterica-host protein-protein interactions during infection. Cell Host Microbe. 2021;29:1316-1332.e12 pubmed publisher
Kim T, Jeon S, Jang Y, Gotina L, Won J, Ju Y, et al. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med. 2021;53:956-972 pubmed publisher
Piggott V, Lloyd S, Matchynski J, Perrine S, Conti A. Traumatic Stress, Chronic Ethanol Exposure, or the Combination, Alter Cannabinoid System Components in Reward and Limbic Regions of the Mouse Brain. Molecules. 2021;26: pubmed publisher
van den Boomen D, Sienkiewicz A, Berlin I, Jongsma M, van Elsland D, Luzio J, et al. A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export. Nat Commun. 2020;11:5559 pubmed publisher
Jiang D, Lee H, Pardridge W. Plasmid DNA gene therapy of the Niemann-Pick C1 mouse with transferrin receptor-targeted Trojan horse liposomes. Sci Rep. 2020;10:13334 pubmed publisher
Colaco A, Fernández Suárez M, Shepherd D, Gal L, Bibi C, Chuartzman S, et al. Unbiased yeast screens identify cellular pathways affected in Niemann-Pick disease type C. Life Sci Alliance. 2020;3: pubmed publisher
Eriksson I, Wäster P, Ollinger K. Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins. Cell Death Dis. 2020;11:370 pubmed publisher
Brogden G, Shammas H, Walters F, Maalouf K, Das A, Naim H, et al. Different Trafficking Phenotypes of Niemann-Pick C1 Gene Mutations Correlate with Various Alterations in Lipid Storage, Membrane Composition and Miglustat Amenability. Int J Mol Sci. 2020;21: pubmed publisher
Dardis A, Zampieri S, Gellera C, Carrozzo R, Cattarossi S, Peruzzo P, et al. Molecular Genetics of Niemann-Pick Type C Disease in Italy: An Update on 105 Patients and Description of 18 NPC1 Novel Variants. J Clin Med. 2020;9: pubmed publisher
Tonini C, Colardo M, Colella B, Di Bartolomeo S, Berardinelli F, Caretti G, et al. Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis. Int J Mol Sci. 2020;21: pubmed publisher
Mitroi D, Pereyra Gómez G, Soto Huelin B, Senovilla F, Kobayashi T, Esteban J, et al. NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation. EMBO Rep. 2019;20:e48143 pubmed publisher
Heybrock S, Kanerva K, Meng Y, Ing C, Liang A, Xiong Z, et al. Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export. Nat Commun. 2019;10:3521 pubmed publisher
Vacca F, Vossio S, Mercier V, Moreau D, Johnson S, Scott C, et al. Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells. J Lipid Res. 2019;60:832-843 pubmed publisher
Xu C, Zhang Y, Rolfe P, Hernández V, Guzman W, Kradjian G, et al. Combination Therapy with NHS-muIL12 and Avelumab (anti-PD-L1) Enhances Antitumor Efficacy in Preclinical Cancer Models. Clin Cancer Res. 2017;23:5869-5880 pubmed publisher
Ebrahimi Fakhari D, Wahlster L, Bartz F, Werenbeck Ueding J, Praggastis M, Zhang J, et al. Reduction of TMEM97 increases NPC1 protein levels and restores cholesterol trafficking in Niemann-pick type C1 disease cells. Hum Mol Genet. 2016;25:3588-3599 pubmed publisher
Ferrante A, De Nuccio C, Pepponi R, Visentin S, Martire A, Bernardo A, et al. Stimulation of adenosine A2A receptors reduces intracellular cholesterol accumulation and rescues mitochondrial abnormalities in human neural cell models of Niemann-Pick C1. Neuropharmacology. 2016;103:155-62 pubmed publisher
Li J, Deffieu M, Lee P, Saha P, Pfeffer S. Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells. Proc Natl Acad Sci U S A. 2015;112:14876-81 pubmed publisher
Lao T, Glass K, Qiu W, Polverino F, Gupta K, Morrow J, et al. Haploinsufficiency of Hedgehog interacting protein causes increased emphysema induced by cigarette smoke through network rewiring. Genome Med. 2015;7:12 pubmed publisher
Kwilas A, Ardiani A, Donahue R, Aftab D, Hodge J. Dual effects of a targeted small-molecule inhibitor (cabozantinib) on immune-mediated killing of tumor cells and immune tumor microenvironment permissiveness when combined with a cancer vaccine. J Transl Med. 2014;12:294 pubmed publisher
product information
master code :
NB400-148
SKU :
NB400-148
product name :
Niemann-Pick C1 Antibody - BSA Free
unit size :
0.1 ml (also 0.025 ml)
description :
The Niemann-Pick C1 Antibody - BSA Free from Novus is a rabbit polyclonal antibody to Niemann-Pick C1. This antibody reacts with chinese hamster,hamster,human,mouse,porcine,primate,rat,hamster - cricetulus (chinese hamster). The Niemann-Pick C1 Antibody - BSA Free has been validated for the following applications: Western Blot,Immunocytochemistry/ Immunofluorescence,IF/IHC,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunoblotting,Electron Microscopy,Knockdown Validated,Block/Neutralize,Immunohistochemistry,Knockout Validated.
target :
Niemann-Pick C1
category :
Primary Antibodies
buffer :
PBS
clonality :
Polyclonal
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Rabbit
immunogen :
A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
isotype :
IgG
purity :
Immunogen affinity purified
species :
Chinese Hamster,Hamster,Human,Mouse,Porcine,Primate,Rat,Hamster - Cricetulus (Chinese Hamster)
gene symbol :
NPC1
Antibody validation :
Biological Validation
accessionNumbers :
O15118
applications :
Western Blot,IF/IHC,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunoblotting,Electron Microscopy,Knockdown Validated,Block/Neutralize,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Knockout Validated
USD :
399 USD
alt names :
FLJ98532, Niemann-Pick C1 protein, Niemann-Pick disease, type C1, NPC, NPC1
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.