product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
alpha-Smooth Muscle Actin Antibody
catalog :
NB300-978
quantity :
0.1 mg (also 25 ug)
price :
469 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
clone name :
2A4
reactivity :
human, mouse, rat, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 95
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3a
Lassiter R, Merchen T, Fang X, Wang Y. Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation. Front Immunol. 2021;12:671025 pubmed publisher
  • immunohistochemistry; human; 1:2000; loading ...; fig 1c
Hsieh Y, Lee K, Lei H, Lan K, Huo T, Lin Y, et al. (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway. Cell Mol Gastroenterol Hepatol. 2021;12:813-838 pubmed publisher
  • western blot; domestic rabbit; 1:100; loading ...; fig 4a
Saikia P, Thangavadivel S, Medeiros C, Lassance L, de Oliveira R, Wilson S. IL-1 and TGF-β Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells. Invest Ophthalmol Vis Sci. 2018;59:5589-5598 pubmed publisher
McMillan I, Gearing M, Wang L. Vascular Heparan Sulfate and Amyloid-β in Alzheimer's Disease Patients. Int J Mol Sci. 2024;25: pubmed publisher
Simkin J, Aloysius A, Adam M, Safaee F, Donahue R, Biswas S, et al. Tissue-resident macrophages specifically express Lactotransferrin and Vegfc during ear pinna regeneration in spiny mice. Dev Cell. 2024;59:496-516.e6 pubmed publisher
Fang Z, Wang Z, Chen Z, Peng Y, Fu Y, Yang Y, et al. Fine particulate matter contributes to COPD-like pathophysiology: experimental evidence from rats exposed to diesel exhaust particles. Respir Res. 2024;25:14 pubmed publisher
Kim D, Goo B, Shi H, Coffey P, Veerapaneni P, Chouhaita R, et al. Integrative multiomics analysis of neointima proliferation in human saphenous vein: implications for bypass graft disease. bioRxiv. 2023;: pubmed publisher
Fu Y, Liu H, Li K, Wei P, Alam N, Deng J, et al. C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms. Front Immunol. 2023;14:1233807 pubmed publisher
More H, Braam B, Cupples W. Reduced tubuloglomerular feedback activity and absence of its synchronization in a connexin40 knockout rat. Front Netw Physiol. 2023;3:1208303 pubmed publisher
Shiju T, Sampaio L, Martinez V, Hilgert G, Wilson S. Transforming growth factor beta-3 localization in the corneal response to epithelial-stromal injury and effects on corneal fibroblast transition to myofibroblasts. Exp Eye Res. 2023;235:109631 pubmed publisher
Jeong J, Tanaka M, Yang Y, Arefyev N, DiRito J, Tietjen G, et al. An optimized visualization and quantitative protocol for in-depth evaluation of lymphatic vessel architecture in the liver. Am J Physiol Gastrointest Liver Physiol. 2023;325:G379-G390 pubmed publisher
Xu M, Tan J, Zhu L, Ge C, Zhang Y, Gao F, et al. Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S-Palmitoylated IRHOM2. Adv Sci (Weinh). 2023;10:e2302130 pubmed publisher
Kersten V, Seitz T, Sommer J, Thasler W, Bosserhoff A, Hellerbrand C. Bone Morphogenetic Protein 13 Has Protumorigenic Effects on Hepatocellular Carcinoma Cells In Vitro. Int J Mol Sci. 2023;24: pubmed publisher
Assini J, Clark J, Youssef A, Xing C, Doerfler A, Park S, et al. High levels of lipoprotein(a) in transgenic mice exacerbate atherosclerosis and promote vulnerable plaque features in a sex-specific manner. Atherosclerosis. 2023;384:117150 pubmed publisher
Szab xf3 V, Cs xe1 k xe1 ny Papp N, G xf6 r xf6 g M, Mad xe1 csy T, Varga x, Kiss A, et al. Orai1 calcium channel inhibition prevents progression of chronic pancreatitis. JCI Insight. 2023;8: pubmed publisher
Li S, Gao Z, Li H, Xu C, Chen B, Zha Q, et al. Hif-1α/Slit2 Mediates Vascular Smooth Muscle Cell Phenotypic Changes in Restenosis of Bypass Grafts. J Cardiovasc Transl Res. 2023;16:1021-1031 pubmed publisher
Dona M, Hsu I, Meuth A, Brown S, Bailey C, Aragonez C, et al. Multi-omic analysis of the cardiac cellulome defines a vascular contribution to cardiac diastolic dysfunction in obese female mice. Basic Res Cardiol. 2023;118:11 pubmed publisher
Yang A, Kim Y, Yan X, Abe H, Aslam M, Park K, et al. Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis. Cell Mol Gastroenterol Hepatol. 2023;15:841-867 pubmed publisher
Itotagawa E, Tomofuji Y, Kato Y, Konaka H, Tsujimoto K, Park J, et al. SLE stratification based on BAFF and IFN-I bioactivity for biologics and implications of BAFF produced by glomeruli in lupus nephritis. Rheumatology (Oxford). 2023;62:1988-1997 pubmed publisher
Guan X, Liu D, Zhou H, Dai C, Wang T, Fang Y, et al. Melatonin improves pregnancy outcomes in adenomyosis mice by restoring endometrial receptivity via NF-κB/apoptosis signaling. Ann Transl Med. 2022;10:1317 pubmed publisher
Latif A, Fisher L, Dundas A, Cuzzucoli Crucitti V, Imir Z, Lawler K, et al. Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing. Adv Mater. 2022;:e2208364 pubmed publisher
Kim D, Jeong J, Lee D, Hyeon D, Park G, Jeon S, et al. PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141+ cancer-associated fibroblasts in pancreatic cancer. Nat Commun. 2022;13:6292 pubmed publisher
Shin H, Thakore A, Tada Y, Pedroza A, Ikeda G, Chen I, et al. Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling. Sci Rep. 2022;12:17605 pubmed publisher
Mir S, Golden B, Griess B, Vengoji R, Tom E, Kosmacek E, et al. Upregulation of Nox4 induces a pro-survival Nrf2 response in cancer-associated fibroblasts that promotes tumorigenesis and metastasis, in part via Birc5 induction. Breast Cancer Res. 2022;24:48 pubmed publisher
Ikeda S, Sugioka S, Kimura T, Ashida N. Smooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors. Sci Rep. 2022;12:11564 pubmed publisher
Liu H, Tian K, Xia C, Wei P, Xu B, Fu W, et al. Kunming mouse strain is less susceptible to elastase-induced abdominal aortic aneurysms. Animal Model Exp Med. 2022;5:72-80 pubmed publisher
Verma S, Deshmukh V, Thatcher K, Belanger K, Rhyner A, Meng S, et al. RBFOX2 is required for establishing RNA regulatory networks essential for heart development. Nucleic Acids Res. 2022;50:2270-2286 pubmed publisher
Ichiki T, Wang T, Kennedy A, Pool A, Ebisu H, Anderson D, et al. Sensory representation and detection mechanisms of gut osmolality change. Nature. 2022;602:468-474 pubmed publisher
Wu Q, Huang Q, Jiang Y, Sun F, Liang B, Wang J, et al. Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability. Cancer Immunol Res. 2022;10:182-199 pubmed publisher
Link P, Choi K, Diaz Espinosa A, Jones D, Gao A, Haak A, et al. Combined control of the fibroblast contractile program by YAP and TAZ. Am J Physiol Lung Cell Mol Physiol. 2022;322:L23-L32 pubmed publisher
Geng X, Xu Z, Tu C, Peng J, Jin X, Ye L, et al. Hydrogel Complex Electrospun Scaffolds and Their Multiple Functions in In Situ Vascular Tissue Engineering. ACS Appl Bio Mater. 2021;4:2373-2384 pubmed publisher
Tian K, Xia C, Liu H, Xu B, Wei P, Fu W, et al. Temporal and Quantitative Analysis of Aortic Immunopathologies in Elastase-Induced Mouse Abdominal Aortic Aneurysms. J Immunol Res. 2021;2021:6297332 pubmed publisher
Sampaio L, Shiju T, Hilgert G, de Oliveira R, DeDreu J, Menko A, et al. Descemet's membrane injury and regeneration, and posterior corneal fibrosis, in rabbits. Exp Eye Res. 2021;213:108803 pubmed publisher
Wang H, Hironaka C, Mullis D, Lucian H, Shin H, Tran N, et al. A neonatal leporine model of age-dependent natural heart regeneration after myocardial infarction. J Thorac Cardiovasc Surg. 2021;: pubmed publisher
Tian J, Zhang C, Kang N, Wang J, Kong N, Zhou J, et al. Attenuated monoamine oxidase a impairs endometrial receptivity in women with adenomyosis via downregulation of FOXO1†. Biol Reprod. 2021;105:1443-1457 pubmed publisher
Kim D, Yoon Y, Choi D, Kim J, Lim J. 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis. Front Cell Dev Biol. 2021;9:698292 pubmed publisher
Weder B, Schefer F, Van Haaften W, Patsenker E, Stickel F, Mueller S, et al. New Therapeutic Approach for Intestinal Fibrosis Through Inhibition of pH-Sensing Receptor GPR4. Inflamm Bowel Dis. 2021;: pubmed publisher
Smith A, Akdemir G, Wadhwa M, Song D, Verkman A. Application of fluorescent dextrans to the brain surface under constant pressure reveals AQP4-independent solute uptake. J Gen Physiol. 2021;153: pubmed publisher
Beylina A, Langston R, Rosen D, Reed X, Cookson M. Generation of fourteen isogenic cell lines for Parkinson's disease-associated leucine-rich repeat kinase (LRRK2). Stem Cell Res. 2021;53:102354 pubmed publisher
Yildirim M, Oztay F, Kayalar O, Tasci A. Effect of long noncoding RNAs on epithelial-mesenchymal transition in A549 cells and fibrotic human lungs. J Cell Biochem. 2021;122:882-896 pubmed publisher
Huang N, Perez P, Kato T, Mikami Y, Okuda K, Gilmore R, et al. SARS-CoV-2 infection of the oral cavity and saliva. Nat Med. 2021;27:892-903 pubmed publisher
Zhang Y, Recouvreux M, Jung M, Galenkamp K, Li Y, Zagnitko O, et al. Macropinocytosis in Cancer-Associated Fibroblasts Is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness. Cancer Discov. 2021;11:1808-1825 pubmed publisher
Yang Z, Wang W, Zhao L, Wang X, Gimple R, Xu L, et al. Plasma cells shape the mesenchymal identity of ovarian cancers through transfer of exosome-derived microRNAs. Sci Adv. 2021;7: pubmed publisher
de Oliveira R, Tye G, Sampaio L, Shiju T, DeDreu J, Menko A, et al. TGFβ1 and TGFβ2 proteins in corneas with and without stromal fibrosis: Delayed regeneration of apical epithelial growth factor barrier and the epithelial basement membrane in corneas with stromal fibrosis. Exp Eye Res. 2020;:108325 pubmed publisher
Marchetto N, Begum S, Wu T, O Besso V, Yarborough C, Valero Pacheco N, et al. Endothelial Jagged1 Antagonizes Dll4/Notch Signaling in Decidual Angiogenesis during Early Mouse Pregnancy. Int J Mol Sci. 2020;21: pubmed publisher
Ucar B, Yusufogullari S, Humpel C. Collagen hydrogels loaded with fibroblast growth factor-2 as a bridge to repair brain vessels in organotypic brain slices. Exp Brain Res. 2020;238:2521-2529 pubmed publisher
de Oliveira R, Murillo S, Saikia P, Wilson S. The Efficacy of Topical HGF on Corneal Fibrosis and Epithelial Healing after Scar-Producing PRK Injury in Rabbits. Transl Vis Sci Technol. 2020;9:29 pubmed publisher
Bosc C, Broin N, Fanjul M, Saland E, Farge T, Courdy C, et al. Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites. Nat Commun. 2020;11:4056 pubmed publisher
Chevalier J, Yin H, Arpino J, O Neil C, Nong Z, Gilmore K, et al. Obstruction of Small Arterioles in Patients with Critical Limb Ischemia due to Partial Endothelial-to-Mesenchymal Transition. iScience. 2020;23:101251 pubmed publisher
Kirst C, Skriabine S, Vieites Prado A, Topilko T, Bertin P, Gerschenfeld G, et al. Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature. Cell. 2020;180:780-795.e25 pubmed publisher
Park Y, An M, Kim J, Lim Y. Antiobesity effect of ethanolic extract of Ramulus mori in differentiated 3T3-L1 adipocytes and high-fat diet-induced obese mice. J Ethnopharmacol. 2020;251:112542 pubmed publisher
Singh M, Goldsmith M, Thakur K, Chatterjee S, Landesman Milo D, Levy T, et al. An ovarian spheroid based tumor model that represents vascularized tumors and enables the investigation of nanomedicine therapeutics. Nanoscale. 2020;12:1894-1903 pubmed publisher
Sengupta M, Griffett K, Flaveny C, Burris T. Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease. ACS Pharmacol Transl Sci. 2018;1:50-60 pubmed publisher
Gravesteijn G, Munting L, Overzier M, Mulder A, Hegeman I, Derieppe M, et al. Progression and Classification of Granular Osmiophilic Material (GOM) Deposits in Functionally Characterized Human NOTCH3 Transgenic Mice. Transl Stroke Res. 2020;11:517-527 pubmed publisher
Lin R, Jia Y, Wu F, Meng Y, Sun Q, Jia L. Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats. Int J Environ Res Public Health. 2019;16: pubmed publisher
Karhadkar T, Chen W, Gomer R. Attenuated pulmonary fibrosis in sialidase-3 knockout (Neu3-/-) mice. Am J Physiol Lung Cell Mol Physiol. 2020;318:L165-L179 pubmed publisher
Gouw A, Margulis K, Liu N, Raman S, Mancuso A, Toal G, et al. The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth. Cell Metab. 2019;30:556-572.e5 pubmed publisher
Inoue K, Kishimoto S, Akimoto K, Sakuma M, Toyoda S, Inoue T, et al. Cancer-associated fibroblasts show heterogeneous gene expression and induce vascular endothelial growth factor A (VEGFA) in response to environmental stimuli. Ann Gastroenterol Surg. 2019;3:416-425 pubmed publisher
Yang N, Li C, Li H, Liu M, Cai X, Cao F, et al. Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. Front Pharmacol. 2019;10:709 pubmed publisher
Pham D, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells. Cell Death Dis. 2019;10:537 pubmed publisher
Li Y, Xu Y, Jadhav K, Zhu Y, Yin L, Zhang Y. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis. Arterioscler Thromb Vasc Biol. 2019;39:1574-1587 pubmed publisher
Chuang W, Liu Y, Huang C, Lo C, Yao H, Chen H, et al. Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet. J Agric Food Chem. 2019;67:7136-7146 pubmed publisher
Medeiros C, Saikia P, de Oliveira R, Lassance L, Santhiago M, Wilson S. Descemet's Membrane Modulation of Posterior Corneal Fibrosis. Invest Ophthalmol Vis Sci. 2019;60:1010-1020 pubmed publisher
Vadasz Z, Balbir Gurman A, Meroni P, Farge D, Levi Y, Ingegnoli F, et al. Lysyl oxidase-a possible role in systemic sclerosis-associated pulmonary hypertension: a multicentre study. Rheumatology (Oxford). 2019;58:1547-1555 pubmed publisher
Li C, Yen C, Fan C, Chuang W, Huang C, Chen H, et al. 14-Deoxy-11,12-didehydroandrographolide suppresses adipogenesis of 3 T3-L1 preadipocytes by inhibiting CCAAT/enhancer-binding protein β activation and AMPK-mediated mitotic clonal expansion. Toxicol Appl Pharmacol. 2018;359:82-90 pubmed publisher
Ikenoue T, Terakado Y, Zhu C, Liu X, Ohsugi T, Matsubara D, et al. Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation. Sci Rep. 2018;8:2021 pubmed publisher
Khound R, Shen J, Song Y, Santra D, Su Q. Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway. Mol Nutr Food Res. 2017;: pubmed publisher
Thompson J, Wilson P, Wyllie A, Wyllie A, Tannock L. Elevated circulating TGF-β is not the cause of increased atherosclerosis development in biglycan deficient mice. Atherosclerosis. 2018;268:68-75 pubmed publisher
Thompson J, Wilson P, Shridas P, Ji A, de Beer M, de Beer F, et al. Serum amyloid A3 is pro-atherogenic. Atherosclerosis. 2018;268:32-35 pubmed publisher
Yang Y, Yang Q, Yang J, Ma Y, Ding G. Angiotensin II induces cholesterol accumulation and injury in podocytes. Sci Rep. 2017;7:10672 pubmed publisher
Ahmad F, Patrick S, Sheikh T, Sharma V, Pathak P, Malgulwar P, et al. Telomerase reverse transcriptase (TERT) - enhancer of zeste homolog 2 (EZH2) network regulates lipid metabolism and DNA damage responses in glioblastoma. J Neurochem. 2017;143:671-683 pubmed publisher
Machuca Parra A, Bigger Allen A, Sanchez A, Boutabla A, Cardona Vélez J, Amarnani D, et al. Therapeutic antibody targeting of Notch3 signaling prevents mural cell loss in CADASIL. J Exp Med. 2017;214:2271-2282 pubmed publisher
Janssens S, Ciapaite J, Wolters J, van Riel N, Nicolay K, Prompers J. An In Vivo Magnetic Resonance Spectroscopy Study of the Effects of Caloric and Non-Caloric Sweeteners on Liver Lipid Metabolism in Rats. Nutrients. 2017;9: pubmed publisher
Cho S, Roh K, Park J, Park Y, Lee M, Cho S, et al. Hydrolysis of Hyaluronic Acid in Lymphedematous Tissue Alleviates Fibrogenesis via TH1 Cell-Mediated Cytokine Expression. Sci Rep. 2017;7:35 pubmed publisher
Hutter Schmid B, Humpel C. Alpha-Smooth Muscle Actin mRNA and Protein Are Increased in Isolated Brain Vessel Extracts of Alzheimer Mice. Pharmacology. 2016;98:251-260 pubmed
Dungan C, Li J, Williamson D. Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling. Lipids. 2016;51:905-12 pubmed publisher
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Gosmain Y, Dif N, Berbe V, Loizon E, Rieusset J, Vidal H, et al. Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues. J Lipid Res. 2005;46:697-705 pubmed
Zhang Y, Hillgartner F. Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens. J Nutr. 2004;134:2205-10 pubmed
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product information
master code :
NB300-978
SKU :
NB300-978
product name :
alpha-Smooth Muscle Actin Antibody
unit size :
0.1 mg (also 25 ug)
description :
The alpha-Smooth Muscle Actin Antibody from Novus is a goat polyclonal antibody to alpha-Smooth Muscle Actin. This antibody reacts with human,mouse,rabbit,rat. The alpha-Smooth Muscle Actin Antibody has been validated for the following applications: Immunohistochemistry,Immunohistochemistry-Paraffin,IF/IHC,Western Blot,Immunohistochemistry-Frozen,Immunohistochemistry Free-Floating,Immunocytochemistry/ Immunofluorescence,Peptide ELISA.
target :
alpha-Smooth Muscle Actin
category :
Primary Antibodies
buffer :
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
clonality :
Polyclonal
concentration :
0.5 mg/ml
conjugate :
Unconjugated
host :
Goat
immunogen :
Alpha-Smooth Muscle Actin Antibody is made to a peptide with sequence EEEDSTALVC corresponding to N-Terminus according to NP_001604.1, NP_001135417.1.
isotype :
IgG
purity :
Immunogen affinity purified
species :
Human,Mouse,Rabbit,Rat
specificity :
Variants NP_001604
theoretical molecular weight :
42 kDa
gene symbol :
ACTA2
accessionNumbers :
P62736
applications :
Immunohistochemistry,Immunohistochemistry-Paraffin,IF/IHC,Western Blot,Immunohistochemistry-Frozen,Immunohistochemistry Free-Floating,Immunocytochemistry/ Immunofluorescence,Peptide ELISA
USD :
469 USD
alt names :
AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTSA, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
storage :
Store at -20C. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.