product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
O-GlcNAc Antibody (RL2) - BSA Free
catalog :
NB300-524
quantity :
0.1 ml (also 0.02 ml)
price :
459 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RL2
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, dot blot
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s8d
  • immunocytochemistry; human; 1:200; loading ...; fig s7a
Taparra K, Wang H, Malek R, Lafargue A, Barbhuiya M, Wang X, et al. O-GlcNAcylation is required for mutant KRAS-induced lung tumorigenesis. J Clin Invest. 2018;128:4924-4937 pubmed publisher
Czajewski I, Swain B, Xu J, McDowall L, Ferenbach A, van Aalten D. Rescuable sleep and synaptogenesis phenotypes in a Drosophila model of O-GlcNAc transferase intellectual disability. elife. 2024;13: pubmed publisher
Authier F, Ondruskova N, Ferenbach A, McNeilly A, van Aalten D. Neurodevelopmental defects in a mouse model of O-GlcNAc transferase intellectual disability. Dis Model Mech. 2024;17: pubmed publisher
Omelkov xe1 M, Fenger C, Murray M, Hammer T, Pravata V, Bartual S, et al. An O-GlcNAc transferase pathogenic variant linked to intellectual disability affects pluripotent stem cell self-renewal. Dis Model Mech. 2023;16: pubmed publisher
Feng Z, Wang T, Sun Y, Chen S, Hao H, Du W, et al. Sulforaphane suppresses paraquat-induced oxidative damage in bovine in vitro-matured oocytes through Nrf2 transduction pathway. Ecotoxicol Environ Saf. 2023;254:114747 pubmed publisher
Kadosaka T, Watanabe M, Natsui H, Koizumi T, Nakao M, Koya T, et al. Empagliflozin attenuates arrhythmogenesis in diabetic cardiomyopathy by normalizing intracellular Ca2+ handling in ventricular cardiomyocytes. Am J Physiol Heart Circ Physiol. 2023;324:H341-H354 pubmed publisher
Jo R, Shibata H, Kurihara I, Yokota K, Kobayashi S, Murai Takeda A, et al. Mechanisms of mineralocorticoid receptor-associated hypertension in diabetes mellitus: the role of O-GlcNAc modification. Hypertens Res. 2023;46:19-31 pubmed publisher
Ise H, Araki Y, Song I, Akatsuka G. N-acetylglucosamine-bearing polymers mimicking O-GlcNAc-modified proteins elicit anti-fibrotic activities in myofibroblasts and activated stellate cells. Glycobiology. 2023;33:17-37 pubmed publisher
Kumar A, Yarosz E, Andren A, Zhang L, Lyssiotis C, Chang C. NKT cells adopt a glutamine-addicted phenotype to regulate their homeostasis and function. Cell Rep. 2022;41:111516 pubmed publisher
Zhu W, Palazzo T, Zhou M, Ledee D, Olson H, Pa x161 a Toli x107 L, et al. First comprehensive identification of cardiac proteins with putative increased O-GlcNAc levels during pressure overload hypertrophy. PLoS ONE. 2022;17:e0276285 pubmed publisher
Shi Q, Shen Q, Liu Y, Shi Y, Huang W, Wang X, et al. Increased glucose metabolism in TAMs fuels O-GlcNAcylation of lysosomal Cathepsin B to promote cancer metastasis and chemoresistance. Cancer Cell. 2022;40:1207-1222.e10 pubmed publisher
Wang T, Feng Z, Sun Y, Zhao S, Zou H, Hao H, et al. Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine. Int J Mol Sci. 2022;23: pubmed publisher
Guilloteau E, Coll P, Lu Z, Djouina M, Cazaunau M, Waxin C, et al. Murine in utero exposure to simulated complex urban air pollution disturbs offspring gut maturation and microbiota during intestinal suckling-to-weaning transition in a sex-dependent manner. Part Fibre Toxicol. 2022;19:41 pubmed publisher
Raab S, Very N, Duch xea ne B, Rybarczyk P, Jonckheere N, El Yazidi Belkoura I, et al. Evaluation of the expression of fatty acid synthase and O-GlcNAc transferase in patients with liver cancer by exploration of transcriptome databases and experimental approaches. Oncol Lett. 2022;23:105 pubmed publisher
Ishikita A, Matsushima S, Ikeda S, Okabe K, Nishimura R, Tadokoro T, et al. GFAT2 mediates cardiac hypertrophy through HBP-O-GlcNAcylation-Akt pathway. iScience. 2021;24:103517 pubmed publisher
Ogawa Y, Imamoto N. Methods to separate nuclear soluble fractions reflecting localizations in living cells. iScience. 2021;24:103503 pubmed publisher
Song T, Zou Q, Yan Y, Lv S, Li N, Zhao X, et al. DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation. Cell Rep. 2021;36:109739 pubmed publisher
Muha V, Authier F, Szoke Kovacs Z, Johnson S, Gallagher J, McNeilly A, et al. Loss of O-GlcNAcase catalytic activity leads to defects in mouse embryogenesis. J Biol Chem. 2021;296:100439 pubmed publisher
Murakami K, Kurotaki D, Kawase W, Soma S, Fukuchi Y, Kunimoto H, et al. OGT Regulates Hematopoietic Stem Cell Maintenance via PINK1-Dependent Mitophagy. Cell Rep. 2021;34:108579 pubmed publisher
Martínez Fábregas J, Wang L, Pohler E, Cozzani A, Wilmes S, Kazemian M, et al. CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci. Cell Rep. 2020;33:108545 pubmed publisher
Decourcelle A, Very N, Djouina M, Loison I, Thevenet J, Body Malapel M, et al. O-GlcNAcylation Links Nutrition to the Epigenetic Downregulation of UNC5A during Colon Carcinogenesis. Cancers (Basel). 2020;12: pubmed publisher
Nomura A, Yokoe S, Tomoda K, Nakagawa T, Martín Romero F, Asahi M. Fluctuation in O-GlcNAcylation inactivates STIM1 to reduce store-operated calcium ion entry via down-regulation of Ser621 phosphorylation. J Biol Chem. 2020;295:17071-17082 pubmed publisher
Mercier T, Bouvet M, Dubois Deruy E, Dechaumes A, Beseme O, Richard V, et al. Interplay Between Phosphorylation and O-GlcNAcylation of Sarcomeric Proteins in Ischemic Heart Failure. Front Endocrinol (Lausanne). 2018;9:598 pubmed publisher
Taub D, Awal M, Gabel C. O-GlcNAc Signaling Orchestrates the Regenerative Response to Neuronal Injury in Caenorhabditis elegans. Cell Rep. 2018;24:1931-1938.e3 pubmed publisher
Hugo S, Schlegel A. A Genetic Model to Study Increased Hexosamine Biosynthetic Flux. Endocrinology. 2017;158:2420-2426 pubmed publisher
Shin G, Kang H, Lee A, Kim K. Hepatitis B virus-triggered autophagy targets TNFRSF10B/death receptor 5 for degradation to limit TNFSF10/TRAIL response. Autophagy. 2016;12:2451-2466 pubmed
Swamy M, Pathak S, Grzes K, Damerow S, Sinclair L, van Aalten D, et al. Glucose and glutamine fuel protein O-GlcNAcylation to control T cell self-renewal and malignancy. Nat Immunol. 2016;17:712-20 pubmed publisher
Allen J, Crowder R, Crowder R, El Deiry W. First-In-Class Small Molecule ONC201 Induces DR5 and Cell Death in Tumor but Not Normal Cells to Provide a Wide Therapeutic Index as an Anti-Cancer Agent. PLoS ONE. 2015;10:e0143082 pubmed publisher
Liu Y, Hawkins O, Vilgelm A, Pawlikowski J, Ecsedy J, Sosman J, et al. Combining an Aurora Kinase Inhibitor and a Death Receptor Ligand/Agonist Antibody Triggers Apoptosis in Melanoma Cells and Prevents Tumor Growth in Preclinical Mouse Models. Clin Cancer Res. 2015;21:5338-48 pubmed publisher
Stateva S, Villalobo A. O-GlcNAcylation of the human epidermal growth factor receptor. Org Biomol Chem. 2015;13:8196-204 pubmed publisher
Foster K, Jane E, Premkumar D, Morales A, Pollack I. NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5. Int J Oncol. 2015;47:506-16 pubmed publisher
Talekar M, Allen J, Dicker D, El Deiry W. ONC201 induces cell death in pediatric non-Hodgkin's lymphoma cells. Cell Cycle. 2015;14:2422-8 pubmed publisher
Shibutani M, Mori T, Miyano T, Miyake M. Removal of O-GlcNAcylation is important for pig preimplantation development. J Reprod Dev. 2015;61:341-50 pubmed publisher
Prabhu V, Allen J, Dicker D, El Deiry W. Small-Molecule ONC201/TIC10 Targets Chemotherapy-Resistant Colorectal Cancer Stem-like Cells in an Akt/Foxo3a/TRAIL-Dependent Manner. Cancer Res. 2015;75:1423-32 pubmed publisher
Itkonen H, Minner S, Guldvik I, Sandmann M, Tsourlakis M, Berge V, et al. O-GlcNAc transferase integrates metabolic pathways to regulate the stability of c-MYC in human prostate cancer cells. Cancer Res. 2013;73:5277-87 pubmed publisher
Dias W, Cheung W, Hart G. O-GlcNAcylation of kinases. Biochem Biophys Res Commun. 2012;422:224-8 pubmed publisher
product information
master code :
NB300-524
SKU :
NB300-524
product name :
O-GlcNAc Antibody (RL2) - BSA Free
unit size :
0.1 ml (also 0.02 ml)
description :
The O-GlcNAc Antibody (RL2) - BSA Free from Novus is a mouse monoclonal antibody to O-GlcNAc. This antibody reacts with bovine,drosophila,fish,hamster,human,mouse,porcine,primate,rat,virus,xenopus. The O-GlcNAc Antibody (RL2) - BSA Free has been validated for the following applications: IF/IHC,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,ELISA,Immunohistochemistry,Dot Blot,Chromatin Immunoprecipitation (ChIP).
target :
O-GlcNAc
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
RL2
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
isotype :
IgG1
purity :
Protein A purified
species :
Bovine,Drosophila,Fish,Hamster,Human,Mouse,Porcine,Primate,Rat,Virus,Xenopus
specificity :
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues
applications :
IF/IHC,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,ELISA,Immunohistochemistry,Dot Blot,Chromatin Immunoprecipitation (ChIP)
USD :
459 USD
alt names :
GlcNAc, O-linked N-acetylglucosamine
storage :
Store at -20C. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.