This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
GAPDH Antibody (2D4A7)
catalog :
NB300-328
quantity :
0.1 ml (also 0.025 ml)
price :
299 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D4A7
reactivity :
human, mouse, rat, swine, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 53
Published Application/Species/Sample/DilutionReference
  • western blot; human
Cuellar T, Barnes D, Nelson C, Tanguay J, Yu S, Wen X, et al. Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates. Nucleic Acids Res. 2015;43:1189-203 pubmed publisher
  • western blot; human
Field M, Kamynina E, Watkins D, Rosenblatt D, Stover P. Human mutations in methylenetetrahydrofolate dehydrogenase 1 impair nuclear de novo thymidylate biosynthesis. Proc Natl Acad Sci U S A. 2015;112:400-5 pubmed publisher
  • western blot; human
Salcher S, Hagenbuchner J, Geiger K, Seiter M, Rainer J, Kofler R, et al. C10ORF10/DEPP, a transcriptional target of FOXO3, regulates ROS-sensitivity in human neuroblastoma. Mol Cancer. 2014;13:224 pubmed publisher
  • western blot; swine
Lin Y, Yang C, Hsu C, Hsu J, Wu S, Lin C, et al. Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes. Transgenic Res. 2015;24:61-71 pubmed publisher
  • western blot; human
Poenitzsch Strong A, Setaluri V, Spiegelman V. MicroRNA-340 as a modulator of RAS-RAF-MAPK signaling in melanoma. Arch Biochem Biophys. 2014;563:118-24 pubmed publisher
  • western blot; human
Speyer C, Hachem A, Assi A, Johnson J, Devries J, Gorski D. Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer. PLoS ONE. 2014;9:e88830 pubmed publisher
  • western blot; human
Banda M, Speyer C, Semma S, Osuala K, Kounalakis N, Torres Torres K, et al. Metabotropic glutamate receptor-1 contributes to progression in triple negative breast cancer. PLoS ONE. 2014;9:e81126 pubmed publisher
  • western blot; mouse
Borghgraef P, Menuet C, Theunis C, Louis J, Devijver H, Maurin H, et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS ONE. 2013;8:e84442 pubmed publisher
  • western blot; human
Lin H, Lin C, Hsiao P, Wang H, Jiang S, Hsu J, et al. Difference in protein expression profile and chemotherapy drugs response of different progression stages of LNCaP sublines and other human prostate cancer cells. PLoS ONE. 2013;8:e82625 pubmed publisher
  • western blot; mouse
Lin H, Hsu F, Baumann B, Coulter D, Lynch D. Cortical synaptic NMDA receptor deficits in ?7 nicotinic acetylcholine receptor gene deletion models: implications for neuropsychiatric diseases. Neurobiol Dis. 2014;63:129-40 pubmed publisher
  • western blot; human
Wang Y, Bettaieb A, Sun C, Deverse J, Radecke C, Mathew S, et al. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress. PLoS ONE. 2013;8:e78322 pubmed publisher
  • western blot; swine
Deverse J, Sandhu A, Mendoza N, Edwards C, Sun C, Simon S, et al. Shear stress modulates VCAM-1 expression in response to TNF-? and dietary lipids via interferon regulatory factor-1 in cultured endothelium. Am J Physiol Heart Circ Physiol. 2013;305:H1149-57 pubmed publisher
  • western blot; human
Shin M, Mizokami A, Kim J, Ofude M, Konaka H, Kadono Y, et al. Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin ?1. Prostate. 2013;73:1159-70 pubmed publisher
  • western blot; rat
Peng H, Chen G, Lai C, Hsieh M, Lin T. Spinal serum-inducible and glucocorticoid-inducible kinase 1 mediates neuropathic pain via kalirin and downstream PSD-95-dependent NR2B phosphorylation in rats. J Neurosci. 2013;33:5227-40 pubmed publisher
  • western blot; human
Storm P, Klausen T, Trulsson M, Ho C S J, Dosnon M, Westergren T, et al. A unifying mechanism for cancer cell death through ion channel activation by HAMLET. PLoS ONE. 2013;8:e58578 pubmed publisher
  • western blot; human
Loke W, Lam K, Chong W, Chew S, Quek A, Lim E, et al. Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers. Free Radic Res. 2012;46:1230-7 pubmed publisher
  • western blot; human
Deverse J, Bailey K, Jackson K, Passerini A. Shear stress modulates RAGE-mediated inflammation in a model of diabetes-induced metabolic stress. Am J Physiol Heart Circ Physiol. 2012;302:H2498-508 pubmed publisher
  • western blot; mouse
Field M, Anderson D, Stover P. Mthfs is an Essential Gene in Mice and a Component of the Purinosome. Front Genet. 2011;2:36 pubmed publisher
  • western blot; human
Andersson M, Lutay N, Hallgren O, Westergren Thorsson G, Svensson M, Godaly G. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses. Innate Immun. 2012;18:531-40 pubmed publisher
  • western blot; domestic rabbit
Johnen S, Kazanskaya O, Armogan N, Stickelmann C, Stocker M, Walter P, et al. Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF. Invest Ophthalmol Vis Sci. 2011;52:5400-7 pubmed publisher
Zhang Q, Narayanan V, Mui K, O Bryan C, Anderson R, Kc B, et al. Mechanical Stabilization of the Glandular Acinus by Linker of Nucleoskeleton and Cytoskeleton Complex. Curr Biol. 2019;29:2826-2839.e4 pubmed publisher
Wu T, Chen C, Lin J, Young G, Wang H, Chen H. The anti-inflammatory function of adenine occurs through AMPK activation and its downstream transcriptional regulation in THP-1 cells. Biosci Biotechnol Biochem. 2019;83:2220-2229 pubmed publisher
McSweeney K, Bozza W, Alterovitz W, Zhang B. Transcriptomic profiling reveals p53 as a key regulator of doxorubicin-induced cardiotoxicity. Cell Death Discov. 2019;5:102 pubmed publisher
Shen C, Huang W, Hsu H, Yang J, Peng C. GAP31 from an ancient medicinal plant exhibits anti-viral activity through targeting to Epstein-Barr virus nuclear antigen 1. Antiviral Res. 2019;164:123-130 pubmed publisher
Huguier V, Giot J, Simonneau M, Levillain P, Charreau S, Garcia M, et al. Oncostatin M exerts a protective effect against excessive scarring by counteracting the inductive effect of TGFβ1 on fibrosis markers. Sci Rep. 2019;9:2113 pubmed publisher
Melissaridou S, Wiechec E, Magan M, Jain M, Chung M, Farnebo L, et al. The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer. Cancer Cell Int. 2019;19:16 pubmed publisher
Twomey J, Zhao L, Luo S, Xu Q, Zhang B. Tubulin couples death receptor 5 to regulate apoptosis. Oncotarget. 2018;9:36804-36815 pubmed publisher
Sexton R, Hachem A, Assi A, Bukhsh M, Gorski D, Speyer C. Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer. Sci Rep. 2018;8:16008 pubmed publisher
Ehyai S, Miyake T, Williams D, Vinayak J, Bayfield M, McDermott J. FMRP recruitment of β-catenin to the translation pre-initiation complex represses translation. EMBO Rep. 2018;19: pubmed publisher
Carrasco Ruiz M, Hernández Aragón L, Chávez Ríos J, Rodríguez Antolín J, Pacheco P, Martínez Gómez M, et al. High Estradiol Differentially Affects the Expression of the Glucose Transporter Type 4 in Pelvic Floor Muscles of Rats. Int Neurourol J. 2018;22:161-168 pubmed publisher
Cao J, Qiu J, Wang X, Lu Z, Wang D, Feng H, et al. Identification of microRNA-124 in regulation of Hepatocellular carcinoma through BIRC3 and the NF-κB pathway. J Cancer. 2018;9:3006-3015 pubmed publisher
Bozza W, Zhang Y, Zhang B. Cytokeratin 8/18 protects breast cancer cell lines from TRAIL-induced apoptosis. Oncotarget. 2018;9:23264-23273 pubmed publisher
Cao J, Yan X, Liu T, Han X, Yu J, Liu S, et al. MicroRNA-552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway in colorectal cancer. Oncol Lett. 2017;14:3795-3802 pubmed publisher
Speyer C, Bukhsh M, Jafry W, Sexton R, Bandyopadhyay S, Gorski D. Riluzole synergizes with paclitaxel to inhibit cell growth and induce apoptosis in triple-negative breast cancer. Breast Cancer Res Treat. 2017;166:407-419 pubmed publisher
Wäster P, Orfanidis K, Eriksson I, Rosdahl I, Seifert O, Ollinger K. UV radiation promotes melanoma dissemination mediated by the sequential reaction axis of cathepsins-TGF-β1-FAP-α. Br J Cancer. 2017;117:535-544 pubmed publisher
Zocchi M, Costa D, Vené R, Tosetti F, Ferrari N, Minghelli S, et al. Zoledronate can induce colorectal cancer microenvironment expressing BTN3A1 to stimulate effector γδ T cells with antitumor activity. Oncoimmunology. 2017;6:e1278099 pubmed publisher
Zhao L, Zhang B. Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosis in cardiomyocytes. Sci Rep. 2017;7:44735 pubmed publisher
Bulanova D, Akimov Y, Rokka A, Laajala T, Aittokallio T, Kouvonen P, et al. Orphan G protein-coupled receptor GPRC5A modulates integrin ?1-mediated epithelial cell adhesion. Cell Adh Migr. 2017;11:434-446 pubmed publisher
Bergström P, Agholme L, Nazir F, Satir T, Toombs J, Wellington H, et al. Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation. Sci Rep. 2016;6:29200 pubmed publisher
Lee H, Chang C, Su C, Huang H, Mehndiratta S, Chao Y, et al. 2-(Phenylsulfonyl)quinoline N-hydroxyacrylamides as potent anticancer agents inhibiting histone deacetylase. Eur J Med Chem. 2016;122:92-101 pubmed publisher
Speyer C, Nassar M, Hachem A, Bukhsh M, Jafry W, Khansa R, et al. Riluzole mediates anti-tumor properties in breast cancer cells independent of metabotropic glutamate receptor-1. Breast Cancer Res Treat. 2016;157:217-228 pubmed publisher
Wei L, Wang H, Yang F, Ding Q, Zhao J. Interleukin-17 potently increases non-small cell lung cancer growth. Mol Med Rep. 2016;13:1673-80 pubmed publisher
Wang Y, Serricchio M, Jauregui M, Shanbhag R, Stoltz T, Di Paolo C, et al. Deubiquitinating enzymes regulate PARK2-mediated mitophagy. Autophagy. 2015;11:595-606 pubmed publisher
Carta S, Penco F, Lavieri R, Martini A, DINARELLO C, Gattorno M, et al. Cell stress increases ATP release in NLRP3 inflammasome-mediated autoinflammatory diseases, resulting in cytokine imbalance. Proc Natl Acad Sci U S A. 2015;112:2835-40 pubmed publisher
Venè R, Cardinali B, Arena G, Ferrari N, Benelli R, Minghelli S, et al. Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress. Neoplasia. 2014;16:710-22 pubmed publisher
Domert J, Rao S, Agholme L, Brorsson A, Marcusson J, Hallbeck M, et al. Spreading of amyloid-? peptides via neuritic cell-to-cell transfer is dependent on insufficient cellular clearance. Neurobiol Dis. 2014;65:82-92 pubmed publisher
Amaro A, Mirisola V, Angelini G, Musso A, Tosetti F, Esposito A, et al. Evidence of epidermal growth factor receptor expression in uveal melanoma: inhibition of epidermal growth factor-mediated signalling by Gefitinib and Cetuximab triggered antibody-dependent cellular cytotoxicity. Eur J Cancer. 2013;49:3353-65 pubmed publisher
Carta S, Silvestri M, Rossi G. Modulation of airway epithelial cell functions by Pidotimod: NF-kB cytoplasmatic expression and its nuclear translocation are associated with an increased TLR-2 expression. Ital J Pediatr. 2013;39:29 pubmed publisher
Benelli R, Stigliani S, Minghelli S, Carlone S, Ferrari N. Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell. Prostate. 2013;73:941-51 pubmed publisher
Benelli R, Vené R, Minghelli S, Carlone S, Gatteschi B, Ferrari N. Celecoxib induces proliferation and Amphiregulin production in colon subepithelial myofibroblasts, activating erk1-2 signaling in synergy with EGFR. Cancer Lett. 2013;328:73-82 pubmed publisher
Ciarlo M, Benelli R, Barbieri O, Minghelli S, Barboro P, Balbi C, et al. Regulation of neuroendocrine differentiation by AKT/hnRNPK/AR/?-catenin signaling in prostate cancer cells. Int J Cancer. 2012;131:582-90 pubmed publisher
Gao J, Cai Q, Lu J, Jha H, Robertson E. Upregulation of cellular Bcl-2 by the KSHV encoded RTA promotes virion production. PLoS ONE. 2011;6:e23892 pubmed publisher
Cheng G, Shao Z, Chaudhari B, Agrawal D. Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2007;293:L1339-47 pubmed
product information
SKU :
NB300-328
product name :
GAPDH Antibody (2D4A7)
unit size :
0.1 ml (also 0.025 ml)
Description :
The GAPDH Antibody (2D4A7) from Novus is a mouse monoclonal antibody to GAPDH. This antibody reacts with human, mouse, rat, porcine, rabbit. The GAPDH Antibody (2D4A7) has been validated for the following applications: Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.
target :
GAPDH
category :
Primary Antibodies
buffer :
PBS
clonality :
Monoclonal
clone :
2D4A7
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
GAPDH antibody raised against Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3). [UniProt# P04406]
isotype :
IgG1 Kappa
purity :
Ammonium sulfate precipitation
species :
Human, Mouse, Rat, Porcine, Rabbit
theoretical molecular weight :
36 kDa
gene symbol :
GAPDH
catalog number base :
NB300-328
accessionNumbers :
P04406
applications :
Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin
2020 Proposedprice USD :
299 USD
alt names :
aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.