product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
GAPDH Antibody (1D4) - BSA Free
catalog :
NB300-221
quantity :
0.1 ml (also 0.025 ml)
price :
479 USD
clonality :
monoclonal
host :
mouse
conjugate :
Alexa Fluor 594
clone name :
1D4
reactivity :
hamsters, primate, human, mouse, rat, dogs, chicken, bovine, pigs , zebrafish , domestic sheep, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 432
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 5d
Xiong L, Zhao K, Cao Y, Guo H, Pan J, Yang X, et al. Linking skeletal muscle aging with osteoporosis by lamin A/C deficiency. PLoS Biol. 2020;18:e3000731 pubmed publisher
  • western blot; mouse; fig 1
Lin J, Kumari S, Kim C, Van T, Wachsmuth L, Polykratis A, et al. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Nature. 2016;540:124-128 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Kümper S, Mardakheh F, McCarthy A, Yeo M, Stamp G, Paul A, et al. Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis. elife. 2016;5:e12994 pubmed publisher
  • western blot; human; 1:1000; fig 3a
Chen Y, Statt S, Wu R, Chang H, Liao J, Wang C, et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep. 2016;6:18815 pubmed publisher
  • western blot; mouse; 1:5000; fig 3
Brai E, Marathe S, Astori S, Fredj N, Perry E, Lamy C, et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015;9:447 pubmed publisher
  • western blot; human; 1:1000
Becs G, Zarjou A, Agarwal A, Kovács K, Becs Ã, Nyitrai M, et al. Pharmacological induction of ferritin prevents osteoblastic transformation of smooth muscle cells. J Cell Mol Med. 2016;20:217-30 pubmed publisher
  • western blot; rat
Zakrzewicz A, Atanasova S, Padberg W, Grau V. Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury. Mediators Inflamm. 2015;2015:429653 pubmed publisher
  • western blot; rat
Chelluboina B, Warhekar A, Dillard M, Klopfenstein J, Pinson D, Wang D, et al. Post-transcriptional inactivation of matrix metalloproteinase-12 after focal cerebral ischemia attenuates brain damage. Sci Rep. 2015;5:9504 pubmed publisher
  • immunocytochemistry; mouse
  • immunohistochemistry; mouse
Marathe S, Liu S, Brai E, Kaczarowski M, Alberi L. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry. Cell Death Differ. 2015;22:1775-84 pubmed publisher
  • western blot; bovine; fig 2
Aboelenain M, Kawahara M, Balboula A, Montasser A, Zaabel S, Okuda K, et al. Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. J Reprod Dev. 2015;61:229-36 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse
Liu Y, Yin J, Barrett B, Sheppard Tillman H, Li D, Casey O, et al. Loss of Androgen-Regulated MicroRNA 1 Activates SRC and Promotes Prostate Cancer Bone Metastasis. Mol Cell Biol. 2015;35:1940-51 pubmed publisher
  • western blot; human
Zhou Q, Derti A, Ruddy D, Rakiec D, Kao I, Lira M, et al. A chemical genetics approach for the functional assessment of novel cancer genes. Cancer Res. 2015;75:1949-58 pubmed publisher
  • western blot; human
Aubry S, Shin W, Crary J, Lefort R, Qureshi Y, Lefebvre C, et al. Assembly and interrogation of Alzheimer's disease genetic networks reveal novel regulators of progression. PLoS ONE. 2015;10:e0120352 pubmed publisher
  • western blot; human
Bergert M, Erzberger A, Desai R, Aspalter I, Oates A, Charras G, et al. Force transmission during adhesion-independent migration. Nat Cell Biol. 2015;17:524-9 pubmed publisher
  • western blot; human
Zakrzewicz A, Kutlimuratov K, Atanasova S, Zakrzewicz D, Reichert M, Schmitz J, et al. Attenuation of acute rat renal allograft rejection by apolipoprotein E-mimetic Peptide. Transplantation. 2015;99:925-34 pubmed publisher
  • western blot; mouse
de Boussac H, Maqdasy S, Trousson A, Zelcer N, Volle D, Lobaccaro J, et al. Enolase is regulated by Liver X Receptors. Steroids. 2015;99:266-71 pubmed publisher
  • western blot; rat
  • western blot; human
Kutz J, Greaney J, Santhanam L, Alexander L. Evidence for a functional vasodilatatory role for hydrogen sulphide in the human cutaneous microvasculature. J Physiol. 2015;593:2121-9 pubmed publisher
  • western blot; rat
  • western blot; human
Li J, Yuan W, Jiang S, Ye W, Yang H, Shapiro I, et al. Prolyl-4-hydroxylase domain protein 2 controls NF-κB/p65 transactivation and enhances the catabolic effects of inflammatory cytokines on cells of the nucleus pulposus. J Biol Chem. 2015;290:7195-207 pubmed publisher
  • western blot; human
Ye W, Zhou J, Markova D, Tian Y, Li J, Anderson D, et al. Xylosyltransferase-1 expression is refractory to inhibition by the inflammatory cytokines tumor necrosis factor α and IL-1β in nucleus pulposus cells: novel regulation by AP-1, Sp1, and Sp3. Am J Pathol. 2015;185:485-95 pubmed publisher
  • western blot; mouse
Liu J, Bain L. Arsenic inhibits hedgehog signaling during P19 cell differentiation. Toxicol Appl Pharmacol. 2014;281:243-53 pubmed publisher
  • western blot; human; fig 6b
Chang S, Chang W, Lu C, Tarn W. Alanine repeats influence protein localization in splicing speckles and paraspeckles. Nucleic Acids Res. 2014;42:13788-98 pubmed publisher
  • western blot; mouse
Zhao N, Koenig S, Trask A, Lin C, Hans C, Garg V, et al. MicroRNA miR145 regulates TGFBR2 expression and matrix synthesis in vascular smooth muscle cells. Circ Res. 2015;116:23-34 pubmed publisher
  • western blot; rat
Peterson J, Mart R, Bond C. Effect of obesity and exercise on the expression of the novel myokines, Myonectin and Fibronectin type III domain containing 5. Peerj. 2014;2:e605 pubmed publisher
  • western blot; human
Domingue J, Ao M, Sarathy J, George A, Alrefai W, Nelson D, et al. HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport. Physiol Rep. 2014;2: pubmed publisher
  • western blot; domestic sheep
Chen X, Sadowska G, Zhang J, Kim J, Cummings E, Bodge C, et al. Neutralizing anti-interleukin-1β antibodies modulate fetal blood-brain barrier function after ischemia. Neurobiol Dis. 2015;73:118-29 pubmed publisher
  • western blot; rat
Wang X, Wang H, Yang H, Li J, Cai Q, Shapiro I, et al. Tumor necrosis factor-?- and interleukin-1?-dependent matrix metalloproteinase-3 expression in nucleus pulposus cells requires cooperative signaling via syndecan 4 and mitogen-activated protein kinase-NF-?B axis: implications in inflammatory disc dis. Am J Pathol. 2014;184:2560-72 pubmed publisher
  • western blot; human
Palsamy P, Bidasee K, Shinohara T. Selenite cataracts: activation of endoplasmic reticulum stress and loss of Nrf2/Keap1-dependent stress protection. Biochim Biophys Acta. 2014;1842:1794-805 pubmed publisher
  • western blot; mouse
  • western blot; rat
  • western blot; human
Alidousty C, Rauen T, Hanssen L, Wang Q, Alampour Rajabi S, Mertens P, et al. Calcineurin-mediated YB-1 dephosphorylation regulates CCL5 expression during monocyte differentiation. J Biol Chem. 2014;289:21401-12 pubmed publisher
  • western blot; mouse
Holmes O, Paturi S, Selkoe D, Wolfe M. Pen-2 is essential for ?-secretase complex stability and trafficking but partially dispensable for endoproteolysis. Biochemistry. 2014;53:4393-406 pubmed publisher
  • western blot; hamsters
McCord M, Kullmann P, He K, Hartnett K, Horn J, Lotan I, et al. Syntaxin-binding domain of Kv2.1 is essential for the expression of apoptotic K+ currents. J Physiol. 2014;592:3511-21 pubmed publisher
  • western blot; rat
Chelluboina B, Klopfenstein J, Pinson D, Wang D, Veeravalli K. Stem cell treatment after cerebral ischemia regulates the gene expression of apoptotic molecules. Neurochem Res. 2014;39:1511-21 pubmed publisher
  • western blot; human
Palsamy P, Bidasee K, Ayaki M, Augusteyn R, Chan J, Shinohara T. Methylglyoxal induces endoplasmic reticulum stress and DNA demethylation in the Keap1 promoter of human lens epithelial cells and age-related cataracts. Free Radic Biol Med. 2014;72:134-48 pubmed publisher
  • western blot; chicken
Wang S, Cheng C, Chen C, Chen H, Tang P, Chen C, et al. Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress. Theriogenology. 2014;82:80-94 pubmed publisher
  • western blot; rat
  • western blot; mouse
Steppan J, Sikka G, Jandu S, Barodka V, Halushka M, Flavahan N, et al. Exercise, vascular stiffness, and tissue transglutaminase. J Am Heart Assoc. 2014;3:e000599 pubmed publisher
  • western blot; mouse; fig 1
Kensler K, Slocum S, Chartoumpekis D, Dolan P, Johnson N, Ilic Z, et al. Genetic or pharmacologic activation of Nrf2 signaling fails to protect against aflatoxin genotoxicity in hypersensitive GSTA3 knockout mice. Toxicol Sci. 2014;139:293-300 pubmed publisher
  • western blot; rat
  • western blot; human
Shah N, Schulien A, Clemens K, Aizenman T, Hageman T, Wills Z, et al. Cyclin e1 regulates Kv2.1 channel phosphorylation and localization in neuronal ischemia. J Neurosci. 2014;34:4326-31 pubmed publisher
  • western blot; mouse
Gokhin D, Tierney M, Sui Z, Sacco A, Fowler V. Calpain-mediated proteolysis of tropomodulin isoforms leads to thin filament elongation in dystrophic skeletal muscle. Mol Biol Cell. 2014;25:852-65 pubmed publisher
  • western blot; human
Sun J, Liu S, Lin Y, Cao X, Liu J. TGF-? promotes glioma cell growth via activating Nodal expression through Smad and ERK1/2 pathways. Biochem Biophys Res Commun. 2014;443:1066-72 pubmed publisher
  • western blot; human
Brosseau J, Lucier J, Nwilati H, Thibault P, Garneau D, Gendron D, et al. Tumor microenvironment-associated modifications of alternative splicing. RNA. 2014;20:189-201 pubmed publisher
  • western blot; human
Bohmer M, Sharbati J, Zur Bruegge J, Einspanier R, Sharbati S. Structural analysis of microRNA-target interaction by sequential seed mutagenesis and stem-loop 3' RACE. PLoS ONE. 2013;8:e81427 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Dickson J, Kruse C, Montagna D, Finsen B, Wolfe M. Alternative polyadenylation and miR-34 family members regulate tau expression. J Neurochem. 2013;127:739-49 pubmed publisher
  • western blot; human
Premaraj S, Souza I, Premaraj T. Focal adhesion kinase mediates ?-catenin signaling in periodontal ligament cells. Biochem Biophys Res Commun. 2013;439:487-92 pubmed publisher
  • western blot; human
Jung S, Jandu S, Steppan J, BELKIN A, An S, Pak A, et al. Increased tissue transglutaminase activity contributes to central vascular stiffness in eNOS knockout mice. Am J Physiol Heart Circ Physiol. 2013;305:H803-10 pubmed publisher
  • western blot; mouse
Hanssen L, Alidousty C, Djudjaj S, Frye B, Rauen T, Boor P, et al. YB-1 is an early and central mediator of bacterial and sterile inflammation in vivo. J Immunol. 2013;191:2604-13 pubmed publisher
  • western blot; rat
Chelluboina B, Klopfenstein J, Gujrati M, Rao J, Veeravalli K. Temporal regulation of apoptotic and anti-apoptotic molecules after middle cerebral artery occlusion followed by reperfusion. Mol Neurobiol. 2014;49:50-65 pubmed publisher
  • western blot; rat
Rostami E, Davidsson J, Gyorgy A, Agoston D, Risling M, Bellander B. The terminal pathway of the complement system is activated in focal penetrating but not in mild diffuse traumatic brain injury. J Neurotrauma. 2013;30:1954-65 pubmed publisher
  • western blot; human
Potor L, Bányai E, Becs G, Soares M, Balla G, Balla J, et al. Atherogenesis may involve the prooxidant and proinflammatory effects of ferryl hemoglobin. Oxid Med Cell Longev. 2013;2013:676425 pubmed publisher
  • western blot; human
Ishimoto T, Cara Fuentes G, Wang H, Shimada M, Wasserfall C, Winter W, et al. Serum from minimal change patients in relapse increases CD80 expression in cultured podocytes. Pediatr Nephrol. 2013;28:1803-12 pubmed publisher
  • western blot; rat
Tian Y, Yuan W, Fujita N, Wang J, Wang H, Shapiro I, et al. Inflammatory cytokines associated with degenerative disc disease control aggrecanase-1 (ADAMTS-4) expression in nucleus pulposus cells through MAPK and NF-?B. Am J Pathol. 2013;182:2310-21 pubmed publisher
  • western blot; pigs
Bosse K, Hans C, Zhao N, Koenig S, Huang N, Guggilam A, et al. Endothelial nitric oxide signaling regulates Notch1 in aortic valve disease. J Mol Cell Cardiol. 2013;60:27-35 pubmed publisher
  • western blot; rat
Krishnamoorthy V, Donofrio A, Martin J. O-GlcNAcylation of ?B-crystallin regulates its stress-induced translocation and cytoprotection. Mol Cell Biochem. 2013;379:59-68 pubmed publisher
  • western blot; human
Caldarelli A, Muller J, Paskowski Rogacz M, Herrmann K, Bauer R, Koch S, et al. A genome-wide RNAi screen identifies proteins modulating aberrant FLT3-ITD signaling. Leukemia. 2013;27:2301-10 pubmed publisher
  • western blot; human
Dhar K, Rakesh K, Pankajakshan D, Agrawal D. SOCS3 promotor hypermethylation and STAT3-NF-?B interaction downregulate SOCS3 expression in human coronary artery smooth muscle cells. Am J Physiol Heart Circ Physiol. 2013;304:H776-85 pubmed publisher
  • western blot; human
Jiang P, Du W, Mancuso A, Wellen K, Yang X. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature. 2013;493:689-93 pubmed publisher
  • western blot; human
Youn H, Kim E, Um S. Zyxin cooperates with PTOV1 to confer retinoic acid resistance by repressing RAR activity. Cancer Lett. 2013;331:192-9 pubmed publisher
  • western blot; human
Fang B, Guo H, Zhang M, Jiang L, Ren F. The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA. FEBS J. 2013;280:1007-17 pubmed publisher
  • western blot; rat
Wang J, Tian Y, Phillips K, Chiverton N, Haddock G, Bunning R, et al. Tumor necrosis factor ?- and interleukin-1?-dependent induction of CCL3 expression by nucleus pulposus cells promotes macrophage migration through CCR1. Arthritis Rheum. 2013;65:832-42 pubmed publisher
  • western blot; mouse
Gokhin D, Nowak R, Kim N, Arnett E, Chen A, Sah R, et al. Tmod1 and CP49 synergize to control the fiber cell geometry, transparency, and mechanical stiffness of the mouse lens. PLoS ONE. 2012;7:e48734 pubmed publisher
  • western blot; human
Jing S, Wang Y, Kuroda M, Su J, Sun G, Liu Q, et al. HIF-1? contributes to hypoxia-induced invasion and metastasis of esophageal carcinoma via inhibiting E-cadherin and promoting MMP-2 expression. Acta Med Okayama. 2012;66:399-407 pubmed
  • western blot; human
Wang B, Sun J, Qian S, Liu X, Zhang S, Liu F, et al. Proliferation and differentiation of osteoblastic cells on silicon-doped TiO(2) film deposited by cathodic arc. Biomed Pharmacother. 2012;66:633-41 pubmed publisher
  • western blot; human
Farjo K, FARJO R, Halsey S, Moiseyev G, Ma J. Retinol-binding protein 4 induces inflammation in human endothelial cells by an NADPH oxidase- and nuclear factor kappa B-dependent and retinol-independent mechanism. Mol Cell Biol. 2012;32:5103-15 pubmed publisher
  • western blot; mouse
Peschard P, McCarthy A, Leblanc Dominguez V, Yeo M, Guichard S, Stamp G, et al. Genetic deletion of RALA and RALB small GTPases reveals redundant functions in development and tumorigenesis. Curr Biol. 2012;22:2063-8 pubmed publisher
  • western blot; human
Miao H, Pan Z, Lei C, Wen J, Li M, Liu Z, et al. Knockdown of GPC3 inhibits the proliferation of Huh7 hepatocellular carcinoma cells through down-regulation of YAP. J Cell Biochem. 2013;114:625-31 pubmed publisher
  • western blot; rat
Brambillasca S, Altkrueger A, Colombo S, Friederich A, Eickelmann P, Mark M, et al. CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells. J Biol Chem. 2012;287:41808-19 pubmed publisher
  • western blot; human
Fang B, Jiang L, Zhang M, Ren F. A novel cell-penetrating peptide TAT-A1 delivers siRNA into tumor cells selectively. Biochimie. 2013;95:251-7 pubmed publisher
  • western blot; domestic sheep
Chen X, Threlkeld S, Cummings E, Juan I, Makeyev O, Besio W, et al. Ischemia-reperfusion impairs blood-brain barrier function and alters tight junction protein expression in the ovine fetus. Neuroscience. 2012;226:89-100 pubmed publisher
  • western blot; human
Elanchezhian R, Palsamy P, Madson C, Lynch D, Shinohara T. Age-related cataracts: homocysteine coupled endoplasmic reticulum stress and suppression of Nrf2-dependent antioxidant protection. Chem Biol Interact. 2012;200:1-10 pubmed publisher
  • western blot; rat
Bates R, Stith B, Stevens K. Chronic central administration of valproic acid: Increased pro-survival phospho-proteins and growth cone associated proteins with no behavioral pathology. Pharmacol Biochem Behav. 2012;103:237-44 pubmed publisher
  • western blot; human
Twigger K, Roulstone V, Kyula J, Karapanagiotou E, Syrigos K, Morgan R, et al. Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway. BMC Cancer. 2012;12:368 pubmed publisher
  • western blot; human
Cheng S, Liu C, Hsu Y, Chang Y, Huang S, Lee J. Expression and biologic significance of adiponectin receptors in papillary thyroid carcinoma. Cell Biochem Biophys. 2013;65:203-10 pubmed publisher
  • western blot; rat
Chu M, Koshman Y, Iyengar R, Kim T, Russell B, Samarel A. Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway. J Signal Transduct. 2012;2012:473410 pubmed publisher
  • western blot; rat
Tzeng B, Chen Y, Huang C, Lin S, Lee K, Chen C. The Ca(v)3.1 T-type calcium channel is required for neointimal formation in response to vascular injury in mice. Cardiovasc Res. 2012;96:533-42 pubmed publisher
  • western blot; rat
Djudjaj S, Chatziantoniou C, Raffetseder U, Guerrot D, Dussaule J, Boor P, et al. Notch-3 receptor activation drives inflammation and fibrosis following tubulointerstitial kidney injury. J Pathol. 2012;228:286-99 pubmed publisher
  • western blot; mouse
Pilar A, Reid Yu S, Cooper C, Mulder D, Coombes B. GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1. PLoS Pathog. 2012;8:e1002773 pubmed publisher
  • western blot; human
Di Y, Zhao J, Harper R. Cigarette smoke induces MUC5AC protein expression through the activation of Sp1. J Biol Chem. 2012;287:27948-58 pubmed publisher
  • western blot; human
Lange C, Brunswig Spickenheier B, Eissing L, Scheja L. Platelet lysate suppresses the expression of lipocalin-type prostaglandin D2 synthase that positively controls adipogenic differentiation of human mesenchymal stromal cells. Exp Cell Res. 2012;318:2284-96 pubmed publisher
  • western blot; mouse
Hong G, Bain L. Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the ?-catenin signaling pathway. Toxicol Sci. 2012;129:146-56 pubmed publisher
  • western blot; human
Shima T, Mizokami A, Miyagi T, Kawai K, Izumi K, Kumaki M, et al. Down-regulation of calcium/calmodulin-dependent protein kinase kinase 2 by androgen deprivation induces castration-resistant prostate cancer. Prostate. 2012;72:1789-801 pubmed publisher
  • western blot; human
  • western blot; rat
Elanchezhian R, Palsamy P, Madson C, Mulhern M, Lynch D, Troia A, et al. Low glucose under hypoxic conditions induces unfolded protein response and produces reactive oxygen species in lens epithelial cells. Cell Death Dis. 2012;3:e301 pubmed publisher
  • western blot; human
Li M, Chen X, Ye Q, Vogt A, Yin X. A high-throughput FRET-based assay for determination of Atg4 activity. Autophagy. 2012;8:401-12 pubmed publisher
  • western blot; human
Lassance L, Miedl H, Konya V, Heinemann A, Ebner B, Hackl H, et al. Differential response of arterial and venous endothelial cells to extracellular matrix is modulated by oxygen. Histochem Cell Biol. 2012;137:641-55 pubmed publisher
  • western blot; mouse
Thomas A, Dunn T, Drayton J, Oort P, Adams S. A high calcium diet containing nonfat dry milk reduces weight gain and associated adipose tissue inflammation in diet-induced obese mice when compared to high calcium alone. Nutr Metab (Lond). 2012;9:3 pubmed publisher
  • western blot; human
Hoeffer C, Sanchez E, Hagerman R, Mu Y, Nguyen D, Wong H, et al. Altered mTOR signaling and enhanced CYFIP2 expression levels in subjects with fragile X syndrome. Genes Brain Behav. 2012;11:332-41 pubmed publisher
  • western blot; human
Zhang K, Yuan K, Wu H, Li Q, Wang Y, Chen S, et al. Identification of potential markers related to neoadjuvant chemotherapy sensitivity of breast cancer by SELDI-TOF MS. Appl Biochem Biotechnol. 2012;166:753-63 pubmed publisher
  • western blot; human
Gokhin D, Kim N, Lewis S, Hoenecke H, D Lima D, Fowler V. Thin-filament length correlates with fiber type in human skeletal muscle. Am J Physiol Cell Physiol. 2012;302:C555-65 pubmed publisher
  • western blot; human
Wei Q, Hariharan V, Huang H. Cell-cell contact preserves cell viability via plakoglobin. PLoS ONE. 2011;6:e27064 pubmed publisher
  • western blot; mouse
Fujiwara R, Chen S, Karin M, Tukey R. Reduced expression of UGT1A1 in intestines of humanized UGT1 mice via inactivation of NF-?B leads to hyperbilirubinemia. Gastroenterology. 2012;142:109-118.e2 pubmed publisher
  • western blot; mouse
Veeravalli K, Ponnala S, Chetty C, Tsung A, Gujrati M, Rao J. Integrin ?9?1-mediated cell migration in glioblastoma via SSAT and Kir4.2 potassium channel pathway. Cell Signal. 2012;24:272-81 pubmed publisher
  • western blot; mouse
Akundi R, Zhi L, Bueler H. PINK1 enhances insulin-like growth factor-1-dependent Akt signaling and protection against apoptosis. Neurobiol Dis. 2012;45:469-78 pubmed publisher
  • western blot; human
Lee J, Liao P, Young K, Chang C, Chen S, Chang T, et al. Identification of hnRNPH1, NF45, and C14orf166 as novel host interacting partners of the mature hepatitis C virus core protein. J Proteome Res. 2011;10:4522-34 pubmed publisher
  • western blot; mouse
Martin P, Liu Y, Pierce R, Abou Kheir W, Casey O, Seng V, et al. Prostate epithelial Pten/TP53 loss leads to transformation of multipotential progenitors and epithelial to mesenchymal transition. Am J Pathol. 2011;179:422-35 pubmed publisher
  • western blot; mouse
Nitzsche A, Paszkowski Rogacz M, Matarese F, Janssen Megens E, Hubner N, Schulz H, et al. RAD21 cooperates with pluripotency transcription factors in the maintenance of embryonic stem cell identity. PLoS ONE. 2011;6:e19470 pubmed publisher
  • western blot; mouse
Jeyasuria P, Subedi K, Suresh A, Condon J. Elevated levels of uterine anti-apoptotic signaling may activate NFKB and potentially confer resistance to caspase 3-mediated apoptotic cell death during pregnancy in mice. Biol Reprod. 2011;85:417-24 pubmed publisher
  • western blot; rat
Tian X, Vroom C, Ghofrani H, Weissmann N, Bieniek E, Grimminger F, et al. Phosphodiesterase 10A upregulation contributes to pulmonary vascular remodeling. PLoS ONE. 2011;6:e18136 pubmed publisher
  • western blot; human
Lee C, Leu S, Tzeng R, Wang S, Tsai S, Sun K, et al. Latent membrane protein 1 of Epstein-Barr virus regulates death-associated protein kinase 1 in lymphoblastoid cell line. Virology. 2011;413:19-25 pubmed publisher
  • immunocytochemistry; mouse
Singaravelu K, Padanilam B. p53 target Siva regulates apoptosis in ischemic kidneys. Am J Physiol Renal Physiol. 2011;300:F1130-41 pubmed publisher
  • western blot; human
Elson Schwab I, Lorentzen A, Marshall C. MicroRNA-200 family members differentially regulate morphological plasticity and mode of melanoma cell invasion. PLoS ONE. 2010;5: pubmed publisher
  • immunohistochemistry - frozen section; chicken
  • western blot; chicken
Ritchey E, Bongini R, Code K, Zelinka C, Petersen Jones S, Fischer A. The pattern of expression of guanine nucleotide-binding protein beta3 in the retina is conserved across vertebrate species. Neuroscience. 2010;169:1376-91 pubmed publisher
  • western blot; mouse
Hirai A, Ohtsuka N, Ikeda T, Taniguchi R, Blau D, Nakagaki K, et al. Role of mouse hepatitis virus (MHV) receptor murine CEACAM1 in the resistance of mice to MHV infection: studies of mice with chimeric mCEACAM1a and mCEACAM1b. J Virol. 2010;84:6654-66 pubmed publisher
  • western blot; human
Côté M, Lavoie J, Houle F, Poirier A, Rousseau S, Huot J. Regulation of vascular endothelial growth factor-induced endothelial cell migration by LIM kinase 1-mediated phosphorylation of annexin 1. J Biol Chem. 2010;285:8013-21 pubmed publisher
  • western blot; human
Chui J, Li M, Di Girolamo N, Chang J, McCluskey P, Wakefield D. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex. Invest Ophthalmol Vis Sci. 2010;51:2558-67 pubmed publisher
  • western blot; primate
Dynia D, Steinmetz A, Kocinsky H. NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol. 2010;298:F745-53 pubmed publisher
  • western blot; human
Holden P, Horton W. Crude subcellular fractionation of cultured mammalian cell lines. BMC Res Notes. 2009;2:243 pubmed publisher
  • western blot; human
Zhu M, Kyprianou N. Role of androgens and the androgen receptor in epithelial-mesenchymal transition and invasion of prostate cancer cells. FASEB J. 2010;24:769-77 pubmed publisher
  • western blot; human
Costford S, Bajpeyi S, Pasarica M, Albarado D, Thomas S, Xie H, et al. Skeletal muscle NAMPT is induced by exercise in humans. Am J Physiol Endocrinol Metab. 2010;298:E117-26 pubmed publisher
  • western blot; mouse
Kang Y, Zhao X, Lovaas J, Eisenberg E, Greene L. Clathrin-independent internalization of normal cellular prion protein in neuroblastoma cells is associated with the Arf6 pathway. J Cell Sci. 2009;122:4062-9 pubmed publisher
  • immunocytochemistry; human
Zhao J, Zhang Y, Ithychanda S, Tu Y, Chen K, Qin J, et al. Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling. J Biol Chem. 2009;284:34308-20 pubmed publisher
  • western blot; human
Kolosionek E, Savai R, Ghofrani H, Weissmann N, Guenther A, Grimminger F, et al. Expression and activity of phosphodiesterase isoforms during epithelial mesenchymal transition: the role of phosphodiesterase 4. Mol Biol Cell. 2009;20:4751-65 pubmed publisher
  • western blot; human
Fox J, Shin W, Caudill M, Stover P. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem. 2009;284:31097-108 pubmed publisher
  • western blot; human
Fox J, Stover P. Mechanism of the internal ribosome entry site-mediated translation of serine hydroxymethyltransferase 1. J Biol Chem. 2009;284:31085-96 pubmed publisher
  • western blot; human
Jung Y, Kim Y, Kim S, Kim S, Kim J, Cho N, et al. Correlation of human leukocyte antigen-G (HLA-G) expression and disease progression in epithelial ovarian cancer. Reprod Sci. 2009;16:1103-11 pubmed publisher
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Catalgol B, Ziaja I, Breusing N, Jung T, Höhn A, Alpertunga B, et al. The proteasome is an integral part of solar ultraviolet a radiation-induced gene expression. J Biol Chem. 2009;284:30076-86 pubmed publisher
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Kim J, Bugaj L, Oh Y, Bivalacqua T, Ryoo S, Soucy K, et al. Arginase inhibition restores NOS coupling and reverses endothelial dysfunction and vascular stiffness in old rats. J Appl Physiol (1985). 2009;107:1249-57 pubmed publisher
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Matsui Y, Hadaschik B, Fazli L, Andersen R, Gleave M, So A. Intravesical combination treatment with antisense oligonucleotides targeting heat shock protein-27 and HTI-286 as a novel strategy for high-grade bladder cancer. Mol Cancer Ther. 2009;8:2402-11 pubmed publisher
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Guerreiro J, Lameu C, Oliveira E, Klitzke C, Melo R, Linares E, et al. Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production. J Biol Chem. 2009;284:20022-33 pubmed publisher
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Sudarshan S, Sourbier C, Kong H, Block K, Valera Romero V, Yang Y, et al. Fumarate hydratase deficiency in renal cancer induces glycolytic addiction and hypoxia-inducible transcription factor 1alpha stabilization by glucose-dependent generation of reactive oxygen species. Mol Cell Biol. 2009;29:4080-90 pubmed publisher
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Ding L, Paszkowski Rogacz M, Nitzsche A, Slabicki M, Heninger A, de Vries I, et al. A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell. 2009;4:403-15 pubmed publisher
  • immunoprecipitation; human
Kang Q, Wang T, Zhang H, Mohandas N, An X. A Golgi-associated protein 4.1B variant is required for assimilation of proteins in the membrane. J Cell Sci. 2009;122:1091-9 pubmed publisher
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Hynynen R, Suchanek M, Spandl J, Bäck N, Thiele C, Olkkonen V. OSBP-related protein 2 is a sterol receptor on lipid droplets that regulates the metabolism of neutral lipids. J Lipid Res. 2009;50:1305-15 pubmed publisher
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Zhao J, Maskrey B, Balzar S, Chibana K, Mustovich A, Hu H, et al. Interleukin-13-induced MUC5AC is regulated by 15-lipoxygenase 1 pathway in human bronchial epithelial cells. Am J Respir Crit Care Med. 2009;179:782-90 pubmed publisher
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Dong C, Kwas C, Wu L. Transcriptional restriction of human immunodeficiency virus type 1 gene expression in undifferentiated primary monocytes. J Virol. 2009;83:3518-27 pubmed publisher
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Devalaraja Narashimha K, Padanilam B. PARP-1 inhibits glycolysis in ischemic kidneys. J Am Soc Nephrol. 2009;20:95-103 pubmed publisher
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Woodruff T, Costantini K, Crane J, Atkin J, Monk P, Taylor S, et al. The complement factor C5a contributes to pathology in a rat model of amyotrophic lateral sclerosis. J Immunol. 2008;181:8727-34 pubmed
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MacFarlane A, Perry C, Girnary H, Gao D, Allen R, Stabler S, et al. Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism. J Biol Chem. 2009;284:1533-9 pubmed publisher
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Klein M, Schermuly R, Ellinghaus P, Milting H, Riedl B, Nikolova S, et al. Combined tyrosine and serine/threonine kinase inhibition by sorafenib prevents progression of experimental pulmonary hypertension and myocardial remodeling. Circulation. 2008;118:2081-90 pubmed publisher
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Singh A, Boldin Adamsky S, Thimmulappa R, Rath S, Ashush H, Coulter J, et al. RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapy. Cancer Res. 2008;68:7975-84 pubmed publisher
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Schmitt R, Marlier A, Cantley L. Zag expression during aging suppresses proliferation after kidney injury. J Am Soc Nephrol. 2008;19:2375-83 pubmed publisher
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Monteghirfo S, Tosetti F, Ambrosini C, Stigliani S, Pozzi S, Frassoni F, et al. Antileukemia effects of xanthohumol in Bcr/Abl-transformed cells involve nuclear factor-kappaB and p53 modulation. Mol Cancer Ther. 2008;7:2692-702 pubmed publisher
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Vené R, Larghero P, Arena G, Sporn M, Albini A, Tosetti F. Glycogen synthase kinase 3beta regulates cell death induced by synthetic triterpenoids. Cancer Res. 2008;68:6987-96 pubmed publisher
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Wee S, Wiederschain D, Maira S, Loo A, Miller C, DeBeaumont R, et al. PTEN-deficient cancers depend on PIK3CB. Proc Natl Acad Sci U S A. 2008;105:13057-62 pubmed publisher
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Smith H, Marra P, Marshall C. uPAR promotes formation of the p130Cas-Crk complex to activate Rac through DOCK180. J Cell Biol. 2008;182:777-90 pubmed publisher
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Alkam T, Nitta A, Mizoguchi H, Itoh A, Murai R, Nagai T, et al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther. 2008;327:137-47 pubmed publisher
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Macartney Coxson D, Hood K, Shi H, Ward T, Wiles A, O Connor R, et al. Metastatic susceptibility locus, an 8p hot-spot for tumour progression disrupted in colorectal liver metastases: 13 candidate genes examined at the DNA, mRNA and protein level. BMC Cancer. 2008;8:187 pubmed publisher
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Turnquist H, Sumpter T, Tsung A, Zahorchak A, Nakao A, Nau G, et al. IL-1beta-driven ST2L expression promotes maturation resistance in rapamycin-conditioned dendritic cells. J Immunol. 2008;181:62-72 pubmed
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Chetty C, Lakka S, Bhoopathi P, Kunigal S, Geiss R, Rao J. Tissue inhibitor of metalloproteinase 3 suppresses tumor angiogenesis in matrix metalloproteinase 2-down-regulated lung cancer. Cancer Res. 2008;68:4736-45 pubmed publisher
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Bass Zubek A, Hobbs R, Amargo E, Garcia N, Hsieh S, Chen X, et al. Plakophilin 2: a critical scaffold for PKC alpha that regulates intercellular junction assembly. J Cell Biol. 2008;181:605-13 pubmed publisher
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Diaz Encarnacion M, Warner G, Gray C, Cheng J, Keryakos H, Nath K, et al. Signaling pathways modulated by fish oil in salt-sensitive hypertension. Am J Physiol Renal Physiol. 2008;294:F1323-35 pubmed publisher
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Zhang Q, Eaton L, Snyder E, Houghtaling S, Mitchell J, Finegold M, et al. Tempol protects against oxidative damage and delays epithelial tumor onset in Fanconi anemia mice. Cancer Res. 2008;68:1601-8 pubmed publisher
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Chen K, Tu Y, Zhang Y, Blair H, Zhang L, Wu C. PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells. J Biol Chem. 2008;283:2508-17 pubmed
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Tsai W, McCormick K, Brazeau D, Brazeau G. Estrogen effects on skeletal muscle insulin-like growth factor 1 and myostatin in ovariectomized rats. Exp Biol Med (Maywood). 2007;232:1314-25 pubmed
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Limesand S, Rozance P, Smith D, Hay W. Increased insulin sensitivity and maintenance of glucose utilization rates in fetal sheep with placental insufficiency and intrauterine growth restriction. Am J Physiol Endocrinol Metab. 2007;293:E1716-25 pubmed publisher
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Cui W, Cuartas E, Ke J, Zhang Q, Einarsson H, Sedgwick J, et al. CD200 and its receptor, CD200R, modulate bone mass via the differentiation of osteoclasts. Proc Natl Acad Sci U S A. 2007;104:14436-41 pubmed
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Woeller C, Fox J, Perry C, Stover P. A ferritin-responsive internal ribosome entry site regulates folate metabolism. J Biol Chem. 2007;282:29927-35 pubmed
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Porchia L, Guerra M, Wang Y, Zhang Y, Espinosa A, Shinohara M, et al. 2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase. Mol Pharmacol. 2007;72:1124-31 pubmed
  • western blot; human
Zhou M, Xia L, Wang J. Metformin transport by a newly cloned proton-stimulated organic cation transporter (plasma membrane monoamine transporter) expressed in human intestine. Drug Metab Dispos. 2007;35:1956-62 pubmed
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Mao W, Fukuoka S, Iwai C, Liu J, Sharma V, Sheu S, et al. Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine. Am J Physiol Heart Circ Physiol. 2007;293:H1636-45 pubmed
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Blight K. Allelic variation in the hepatitis C virus NS4B protein dramatically influences RNA replication. J Virol. 2007;81:5724-36 pubmed publisher
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Redman P, He K, Hartnett K, Jefferson B, Hu L, Rosenberg P, et al. Apoptotic surge of potassium currents is mediated by p38 phosphorylation of Kv2.1. Proc Natl Acad Sci U S A. 2007;104:3568-73 pubmed
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Block K, Gorin Y, Hoover P, Williams P, Chelmicki T, Clark R, et al. NAD(P)H oxidases regulate HIF-2alpha protein expression. J Biol Chem. 2007;282:8019-26 pubmed
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Fatma S, Yakubov R, Anwar K, Hussain M. Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion. J Lipid Res. 2006;47:2422-32 pubmed
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Ju J, Gitcho M, Casmaer C, Patil P, Han D, Spencer S, et al. Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase. Am J Physiol Cell Physiol. 2007;292:C564-72 pubmed
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Kanadia R, Shin J, Yuan Y, Beattie S, Wheeler T, Thornton C, et al. Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy. Proc Natl Acad Sci U S A. 2006;103:11748-53 pubmed
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Shibata Y, Gabbard J, Yamashita M, Tsuji S, Smith M, Nishiyama A, et al. Heat-killed BCG induces biphasic cyclooxygenase 2+ splenic macrophage formation--role of IL-10 and bone marrow precursors. J Leukoc Biol. 2006;80:590-8 pubmed
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Le Boeuf F, Houle F, Sussman M, Huot J. Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor. Mol Biol Cell. 2006;17:3508-20 pubmed
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Barry J, Davidsen M, Limesand S, Galan H, Friedman J, Regnault T, et al. Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction. Exp Biol Med (Maywood). 2006;231:566-75 pubmed
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Azfer A, Niu J, Rogers L, Adamski F, Kolattukudy P. Activation of endoplasmic reticulum stress response during the development of ischemic heart disease. Am J Physiol Heart Circ Physiol. 2006;291:H1411-20 pubmed
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Arthur D, Georgi S, Akassoglou K, Insel P. Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival. J Neurosci. 2006;26:3798-804 pubmed
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Pisitkun T, Bieniek J, Tchapyjnikov D, Wang G, Wu W, Shen R, et al. High-throughput identification of IMCD proteins using LC-MS/MS. Physiol Genomics. 2006;25:263-76 pubmed
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Jia Y, Takimoto K. Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 2006;98:386-93 pubmed
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Godsel L, Hsieh S, Amargo E, Bass A, Pascoe McGillicuddy L, Huen A, et al. Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 2005;171:1045-59 pubmed
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Dusek R, Getsios S, Chen F, Park J, Amargo E, Cryns V, et al. The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes. J Biol Chem. 2006;281:3614-24 pubmed
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Pendleton L, Goodwin B, Solomonson L, Eichler D. Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame. J Biol Chem. 2005;280:24252-60 pubmed
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Sarkar S, Chawla Sarkar M, Young D, Nishiyama K, Rayborn M, Hollyfield J, et al. Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure. J Biol Chem. 2004;279:52630-42 pubmed
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Gonzalez Cabrera P, Shi T, Yun J, McCune D, Rorabaugh B, Perez D. Differential regulation of the cell cycle by alpha1-adrenergic receptor subtypes. Endocrinology. 2004;145:5157-67 pubmed
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Moshynska O, Sankaran K, Pahwa P, Saxena A. Prognostic significance of a short sequence insertion in the MCL-1 promoter in chronic lymphocytic leukemia. J Natl Cancer Inst. 2004;96:673-82 pubmed
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Akassoglou K, Malester B, Xu J, Tessarollo L, Rosenbluth J, Chao M. Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration. Proc Natl Acad Sci U S A. 2004;101:5075-80 pubmed
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Fortun J, Dunn W, Joy S, Li J, Notterpek L. Emerging role for autophagy in the removal of aggresomes in Schwann cells. J Neurosci. 2003;23:10672-80 pubmed
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product information
master code :
NB300-221
SKU :
NB300-221
product name :
GAPDH Antibody (1D4) - BSA Free
unit size :
0.1 ml (also 0.025 ml)
description :
The GAPDH Antibody (1D4) - BSA Free from Novus is a mouse monoclonal antibody to GAPDH. This antibody reacts with avian,avian - chicken,bovine,canine,chicken,chinese hamster,drosophila,equine,feline,hamster,human,insect - drosophila,mouse,opossum,ovine,porcine,primate,rabbit,rat,sheep,zebrafish,fish - danio rerio (zebrafish),frog - xenopus (african clawed frog),hamster - cricetulus (chinese hamster). The GAPDH Antibody (1D4) - BSA Free has been validated for the following applications: Western Blot,Immunocytochemistry/ Immunofluorescence,Immunohistochemistry,Simple Western,Chromatin Immunoprecipitation,Chemotaxis,IF/IHC,Single Cell Western,PCR,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Knockdown Validated.
target :
GAPDH
category :
Primary Antibodies
buffer :
50% PBS, 50% glycerol
clonality :
Monoclonal
clone :
1D4
concentration :
1.0 mg/ml
conjugate :
Unconjugated
host :
Mouse
immunogen :
This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]
isotype :
IgM
purity :
Immunogen affinity purified
species :
Avian,Avian - Chicken,Bovine,Canine,Chicken,Chinese Hamster,Drosophila,Equine,Feline,Hamster,Human,Insect - Drosophila,Mouse,Opossum,Ovine,Porcine,Primate,Rabbit,Rat,Sheep,Zebrafish,Fish - Danio rerio (Zebrafish),Frog - Xenopus (African Clawed Frog),Hamster - Cricetulus (Chinese Hamster)
theoretical molecular weight :
36 kDa
gene symbol :
GAPDH
accessionNumbers :
P00355
applications :
Immunohistochemistry,Western Blot,Simple Western,Immunocytochemistry/ Immunofluorescence,Chromatin Immunoprecipitation,Chemotaxis,IF/IHC,Single Cell Western,PCR,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Knockdown Validated
USD :
479 USD
alt names :
1D4 GAPDH, aging-associated gene 9 protein, Cytoplasm Marker, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, GAPDPeptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38, OCT1 coactivator in S phase, Peptidyl-cysteine S-nitrosylase GAPDH
storage :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.