| Published Application/Species/Sample/Dilution | Reference |
|---|
- immunocytochemistry; human; 1:100; fig 3f
| Li J, Qiu C, Wei Y, Yuan W, Liu J, Cui W, et al. Human Amniotic Epithelial Stem Cell-Derived Retinal Pigment Epithelium Cells Repair Retinal Degeneration. Front Cell Dev Biol. 2021;9:737242 pubmed publisher |
- immunohistochemistry; human; 1:200; fig 4
| Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher |
- immunocytochemistry; dogs; 1:500; loading ...; fig 1b
- western blot; dogs; 1:4000; loading ...; fig 1a
| Uggenti C, Briant K, Streit A, Thomson S, Koay Y, Baines R, et al. Restoration of mutant bestrophin-1 expression, localisation and function in a polarised epithelial cell model. Dis Model Mech. 2016;9:1317-1328 pubmed |
- immunocytochemistry; human
| Bertolotti E, Neri A, Camparini M, Macaluso C, Marigo V. Stem cells as source for retinal pigment epithelium transplantation. Prog Retin Eye Res. 2014;42:130-44 pubmed publisher |
- immunohistochemistry - paraffin section; human
- immunocytochemistry; human
| Sharon D, Al Hamdani S, Engelsberg K, Mizrahi Meissonnier L, Obolensky A, Banin E, et al. Ocular phenotype analysis of a family with biallelic mutations in the BEST1 gene. Am J Ophthalmol. 2014;157:697-709.e1-2 pubmed publisher |
- immunocytochemistry; human
| Cho M, Kim S, Ku S, Park J, Lee H, Yoo D, et al. Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses. Stem Cell Res. 2012;9:101-9 pubmed publisher |
- immunocytochemistry; human
| Krohne T, Westenskow P, Kurihara T, Friedlander D, Lehmann M, Dorsey A, et al. Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells. Stem Cells Transl Med. 2012;1:96-109 pubmed |
| Guziewicz K, Slavik J, Lindauer S, Aguirre G, Zangerl B. Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies. Invest Ophthalmol Vis Sci. 2011;52:4497-505 pubmed publisher |
- immunocytochemistry; human
- western blot; human
| Harness J, Turovets N, Seiler M, Nistor G, Altun G, Agapova L, et al. Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells. PLoS ONE. 2011;6:e14499 pubmed publisher |
| Zhang Y, Patil R, Marmorstein A. Bestrophin 2 is expressed in human non-pigmented ciliary epithelium but not retinal pigment epithelium. Mol Vis. 2010;16:200-6 pubmed |
- immunocytochemistry; human
| Feng Q, Lu S, Klimanskaya I, Gomes I, Kim D, Chung Y, et al. Hemangioblastic derivatives from human induced pluripotent stem cells exhibit limited expansion and early senescence. Stem Cells. 2010;28:704-12 pubmed publisher |
| Buchholz D, Hikita S, Rowland T, Friedrich A, Hinman C, Johnson L, et al. Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells. Stem Cells. 2009;27:2427-34 pubmed publisher |
| Lu B, Malcuit C, Wang S, Girman S, Francis P, Lemieux L, et al. Long-term safety and function of RPE from human embryonic stem cells in preclinical models of macular degeneration. Stem Cells. 2009;27:2126-35 pubmed publisher |
- immunocytochemistry; dogs
- western blot; dogs
| Guziewicz K, Zangerl B, Lindauer S, Mullins R, Sandmeyer L, Grahn B, et al. Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease. Invest Ophthalmol Vis Sci. 2007;48:1959-67 pubmed |
- immunocytochemistry; human
| Klimanskaya I, Hipp J, Rezai K, West M, Atala A, Lanza R. Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics. Cloning Stem Cells. 2004;6:217-45 pubmed |
| Marmorstein L, McLaughlin P, Stanton J, Yan L, Crabb J, Marmorstein A. Bestrophin interacts physically and functionally with protein phosphatase 2A. J Biol Chem. 2002;277:30591-7 pubmed |
- immunohistochemistry - frozen section; human
- immunoprecipitation; human
- immunocytochemistry; human
- western blot; human
- immunohistochemistry - frozen section; pigs
- immunoprecipitation; pigs
- immunocytochemistry; pigs
- western blot; pigs
- immunohistochemistry - frozen section; primate
- immunoprecipitation; primate
- immunocytochemistry; primate
- western blot; primate
| Marmorstein A, Marmorstein L, Rayborn M, Wang X, Hollyfield J, Petrukhin K. Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2000;97:12758-63 pubmed |
| Sripathi S, Hu M, Turaga R, Mertz J, Liu M, Wan J, et al. Proteome Landscape of Epithelial-to-Mesenchymal Transition (EMT) of Retinal Pigment Epithelium Shares Commonalities With Malignancy-Associated EMT. Mol Cell Proteomics. 2021;20:100131 pubmed publisher |
| Afanasyeva T, Corral Serrano J, Garanto A, Roepman R, Cheetham M, Collin R. A look into retinal organoids: methods, analytical techniques, and applications. Cell Mol Life Sci. 2021;78:6505-6532 pubmed publisher |
| Schustak J, Twarog M, Wu X, Wu H, Huang Q, Bao Y. Mechanism of Nucleic Acid Sensing in Retinal Pigment Epithelium (RPE): RIG-I Mediates Type I Interferon Response in Human RPE. J Immunol Res. 2021;2021:9975628 pubmed publisher |
| Choudhury R, Bayatti N, Scharff R, Szula E, Tilakaratna V, Udsen M, et al. FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress. Sci Rep. 2021;11:14175 pubmed publisher |
| Zhao Q, Kong Y, Kittredge A, Li Y, Shen Y, Zhang Y, et al. Distinct expression requirements and rescue strategies for BEST1 loss- and gain-of-function mutations. elife. 2021;10: pubmed publisher |
| Tsai Y, Li Y, Ryu J, Su P, Cheng C, Wu W, et al. Impaired cholesterol efflux in retinal pigment epithelium of individuals with juvenile macular degeneration. Am J Hum Genet. 2021;108:903-918 pubmed publisher |
| D Antonio Chronowska A, D Antonio M, Frazer K. In vitro Differentiation of Human iPSC-derived Retinal Pigment Epithelium Cells (iPSC-RPE). Bio Protoc. 2019;9:e3469 pubmed publisher |
| Biswas P, Borooah S, Matsui H, Voronchikhina M, Zhou J, Zawaydeh Q, et al. Detection and validation of novel mutations in MERTK in a simplex case of retinal degeneration using WGS and hiPSC-RPEs model. Hum Mutat. 2021;42:189-199 pubmed publisher |
| Bonilha V, Bell B, DeBenedictis M, Hagstrom S, Fishman G, Hollyfield J. Cellular Changes in Retinas From Patients With BEST1 Mutations. Front Cell Dev Biol. 2020;8:573330 pubmed publisher |
| Schäfer N, Wolf H, Enzbrenner A, Schikora J, Reichenthaler M, Enzmann V, et al. Properdin Modulates Complement Component Production in Stressed Human Primary Retinal Pigment Epithelium Cells. Antioxidants (Basel). 2020;9: pubmed publisher |
| Mitrousis N, Hacibekiroglu S, Ho M, Sauve Y, Nagy A, van der Kooy D, et al. Hydrogel-mediated co-transplantation of retinal pigmented epithelium and photoreceptors restores vision in an animal model of advanced retinal degeneration. Biomaterials. 2020;257:120233 pubmed publisher |
| Zhang Z, Yan B, Gao F, Li Q, Meng X, Chen P, et al. PSCs Reveal PUFA-Provoked Mitochondrial Stress as a Central Node Potentiating RPE Degeneration in Bietti's Crystalline Dystrophy. Mol Ther. 2020;28:2642-2661 pubmed publisher |
| Udry F, Decembrini S, Gamm D, D xe9 glon N, Kostic C, Arsenijevic Y. Lentiviral mediated RPE65 gene transfer in healthy hiPSCs-derived retinal pigment epithelial cells markedly increased RPE65 mRNA, but modestly protein level. Sci Rep. 2020;10:8890 pubmed publisher |
| Mladenov N, Petrova S, Mladenova K, Bozhinova D, Moskova Doumanova V, Topouzova Hristova T, et al. Miscibility of hBest1 and sphingomyelin in surface films - A prerequisite for interaction with membrane domains. Colloids Surf B Biointerfaces. 2020;189:110893 pubmed publisher |
| Voisin A, Monville C, Plancheron A, Béré E, Gaillard A, Leveziel N. Cathepsin B pH-Dependent Activity Is Involved in Lysosomal Dysregulation in Atrophic Age-Related Macular Degeneration. Oxid Med Cell Longev. 2019;2019:5637075 pubmed publisher |
| Ji C, Li Y, Kittredge A, Hopiavuori A, Ward N, Yao P, et al. Investigation and Restoration of BEST1 Activity in Patient-derived RPEs with Dominant Mutations. Sci Rep. 2019;9:19026 pubmed publisher |
| Smith E, D Antonio Chronowska A, Greenwald W, Borja V, Aguiar L, Pogue R, et al. Human iPSC-Derived Retinal Pigment Epithelium: A Model System for Prioritizing and Functionally Characterizing Causal Variants at AMD Risk Loci. Stem Cell Reports. 2019;12:1342-1353 pubmed publisher |
| Shahi P, Hermans D, Sinha D, Brar S, Moulton H, Stulo S, et al. Gene Augmentation and Readthrough Rescue Channelopathy in an iPSC-RPE Model of Congenital Blindness. Am J Hum Genet. 2019;104:310-318 pubmed publisher |
| Zhang Y, Kittredge A, Ward N, Ji C, Chen S, Yang T. ATP activates bestrophin ion channels through direct interaction. Nat Commun. 2018;9:3126 pubmed publisher |
| Voisin A, Monville C, Plancheron A, Balbous A, Gaillard A, Leveziel N. hRPE cells derived from induced pluripotent stem cells are more sensitive to oxidative stress than ARPE-19 cells. Exp Eye Res. 2018;177:76-86 pubmed publisher |
| Ramachandra Rao S, Pfeffer B, Más Gómez N, Skelton L, Keiko U, Sparrow J, et al. Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome. Autophagy. 2018;14:1796-1817 pubmed publisher |
| Li Y, Zhang Y, Xu Y, Kittredge A, Ward N, Chen S, et al. Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl- currents in human RPE. elife. 2017;6: pubmed publisher |
| Zhang Y, Zhang D, Wei W, Shen B, Wang Y, Zhang Y, et al. Effects of RPE-conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration. Exp Ther Med. 2017;14:3699-3707 pubmed publisher |
| Klingeborn M, Dismuke W, Skiba N, Kelly U, Stamer W, Bowes Rickman C. Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers. Sci Rep. 2017;7:4901 pubmed publisher |
| Shim S, Kim G, Lee D, Lee J, Kwon H, Song W. Survival of Transplanted Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in a Human Recipient for 22 Months. JAMA Ophthalmol. 2017;135:287-289 pubmed publisher |
| Kaneko Y, Pappas C, Tajiri N, Borlongan C. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep. 2016;6:35659 pubmed publisher |
| Parker J, Mitrousis N, Shoichet M. Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro. Biomacromolecules. 2016;17:476-84 pubmed publisher |
| Doumanov J, Zeitz C, Domínguez Giménez P, Audo I, Krishna A, Alfano G, et al. Disease-causing mutations in BEST1 gene are associated with altered sorting of bestrophin-1 protein. Int J Mol Sci. 2013;14:15121-40 pubmed publisher |
| Maruotti J, Wahlin K, Gorrell D, Bhutto I, Lutty G, Zack D. A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells. Stem Cells Transl Med. 2013;2:341-54 pubmed publisher |
| Blenkinsop T, Salero E, Stern J, Temple S. The culture and maintenance of functional retinal pigment epithelial monolayers from adult human eye. Methods Mol Biol. 2013;945:45-65 pubmed publisher |
| Davidson A, Millar I, Burgess Mullan R, Maher G, Urquhart J, Brown P, et al. Functional characterization of bestrophin-1 missense mutations associated with autosomal recessive bestrophinopathy. Invest Ophthalmol Vis Sci. 2011;52:3730-6 pubmed publisher |
| Davidson A, Millar I, Urquhart J, Burgess Mullan R, Shweikh Y, Parry N, et al. Missense mutations in a retinal pigment epithelium protein, bestrophin-1, cause retinitis pigmentosa. Am J Hum Genet. 2009;85:581-92 pubmed publisher |
| Mullins R, Oh K, Heffron E, Hageman G, Stone E. Late development of vitelliform lesions and flecks in a patient with best disease: clinicopathologic correlation. Arch Ophthalmol. 2005;123:1588-94 pubmed |
