Histone H3 [Dimethyl Lys37] Antibody | Novus Biologicals NB21-1272 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

Histone H3 [Dimethyl Lys37] Antibody

catalog :

NB21-1272

quantity :

0.05 mg

price :

349 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, chromatin immunoprecipitation

product information

Catalog Number :

NB21-1272

Product Name :

Histone H3 [Dimethyl Lys37] Antibody

List Price :

349 USD

product type :

Primary Antibodies

Package Size :

0.05 mg

AltNames :

H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783

Buffer :

PBS and 30% Glycerol

Clonality :

Polyclonal

Concentration :

0.39

Application Summary :

Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry/Immunofluorescence, Western Blot

Host :

Rabbit

Immunogen :

Synthetic dimethylated peptide surrounding Lysine 37 of human Histone H3 [Swiss Prot Q71DI3].

Localization :

Nucleus. Chromosome.

Modification :

Dimethyl Lys37

Notes :

0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane. Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.

Preservative :

0.05% Sodium Azide

Purity :

Immunogen affinity purified

CrossReactivity :

C. elegans, Human, Mouse

Storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Swissprot Human :

Q71DI3

Uses :

This Histone H3 K37me2 antibody is useful for is useful for ChIP*, Immunocytochemistry/Immunofluorescence, Dot Blot and Western Blot. In ICC/IF, nuclear staining was observed in HeLa cells, whereas in Western Blot a band is seen ~15kDa in HeLa histone prep, NIH 3T3 histone prep, and C. elegans embryo lysate.