product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal Histone H3 Antibody
catalog :
NB21-1251H
quantity :
0.05 mg
price :
219.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
HRP
reactivity :
human, mouse
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB21-1251H
Product Name :
Rabbit Polyclonal Histone H3 Antibody
List Price :
219.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 mg
AltNames :
H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783
Background :
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Conversion from trimethyl to di- and singly methylated forms also occurs via the transcriptional repressor JHDM3A. H3K36Me1 has been associated with the timing of replication factor Cdc45 association with replicating origins (Pryde et al, PMID 19521516). H3K36Me1 could have important influence over the conserved multiprotein complex and minichromosome maintenance proteins.
Buffer :
PBS
Clonality :
Polyclonal
Conjugate :
HRP
Application Summary :
Western Blot, Dot Blot
Host :
Rabbit
Immunogen :
Synthetic monomethylated peptide surrounding Lysine 36 of human Histone H3.2 [Swiss Prot Q71DI3].
Localization :
Nucleus. Chromosome.
Marker :
Nuclear Marker
Modification :
Monomethyl Lys36
Notes :
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q71DI3
Uses :
This Histone H3 K36me1 antibody is useful for Dot Blot, ChIP*, ICC/IF and Western Blot where a band is seen ~15kDa in HeLa histone prep and C. elegans embryo lysate. In ICC/IF, nuclear staining was observed in HeLa cells.
Research Area :
Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.