product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal Histone H3 Antibody
catalog :
NB21-1132F
quantity :
0.05 mg
price :
209.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
FITC
reactivity :
human, mouse
application :
western blot, chromatin immunoprecipitation
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB21-1132F
Product Name :
Rabbit Polyclonal Histone H3 Antibody
List Price :
209.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 mg
AltNames :
H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783
Background :
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation (PMID 19544422).
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Conjugate :
FITC
Application Summary :
Western Blot, Chromatin Immunoprecipitation
Host :
Rabbit
Immunogen :
Synthetic asymmetric dimethylated peptide surrounding Arginine 17 of human Histone H3.2 [Swiss Prot Q71DI3].
Localization :
Nucleus.
Marker :
Nuclear Marker
Modification :
Asym-dimethyl Arg17
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q71DI3
Uses :
This Histone H3 R17me2a antibody is useful for ChIP, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15 kDa NIH 3T3 histone prep lysate.
Research Area :
Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.