product summary
request information :
company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
Rabbit Polyclonal Histone H3 Antibody
catalog :
NB21-1052F
quantity :
0.05 mg
price :
209.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
FITC
reactivity :
human, mouse
application :
chromatin immunoprecipitation
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB21-1052F
Product Name :
Rabbit Polyclonal Histone H3 Antibody
List Price :
209.00 USD
Product Type :
Primary Antibodies
Package Size :
0.05 mg
AltNames :
H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783
Background :
Methylation of Histone H3 at Lys9 (K9) is an epigenetic silencer of transcription. Gene silencing from histone post translational modifications, as well as DNA methylation, play a key role in the development of normal tissues. If this silencing is disturbed through the artificial silencing of RIZ1, and thereby H3 K9Me1, it has been shown that normal apoptotic processes in precancerous cells can be reduced. Interestingly, data indicates that the conversion of the monomethyl to the trimethyl form requires mediation by SUVR4 in transposons and pseudogenes. Research also indicates that the presence of the G9a/GLP heterodimeric complex is required for this modification to exist. The additional phosphorylation at Thr6 (pT6) affects the ability of other proteins to bind to the H3 tail, along with amplifing the effects of other histone PTMs that are present. Because T6 phosphorylation is constitutive, its dephosphorylation may play a key role in DNA transcription, repair and replication.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Conjugate :
FITC
Application Summary :
Chromatin Immunoprecipitation, Dot Blot
Host :
Rabbit
Immunogen :
Synthetic monomethylated/phosphorylated peptide surrounding Lysine 9 / Threonine 6 of human Histone H3.2 [Swiss Prot Q71DI3].
Localization :
Nucleus. Chromosome
Marker :
Nuclear Marker
Modification :
p Thr6, Monomethyl Lys9
Preservative :
No Preservative
Purity :
Immunogen affinity purified
CrossReactivity :
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Storage :
Store at 4C in the dark.
Swissprot Human :
Q71DI3
Uses :
This Histone H3K9me1/pT6 antibody is useful for ChIP, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15 kDa in HeLa histone prep, NIH 3T3 histone prep, and C. elegans embryo lysate.
Research Area :
Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones, Phospho Specific
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: USA
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.