RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free | Novus Biologicals NB200-598 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

catalog :

NB200-598

quantity :

0.1 ml (also 0.025 ml)

price :

409 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4H8

reactivity :

human, mouse, brewer's yeast

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation

more info or order :

Novus Biologicals product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig 11	Luo W, Chen I, Chen Y, Alkam D, Wang Y, Semenza G. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia. Oncotarget. 2016;7:6379-97 pubmed publisher
immunocytochemistry; mouse	Chan E, Teng G, Corbett E, Choudhury K, Bassing C, Schatz D, et al. Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2. Proc Natl Acad Sci U S A. 2013;110:E4628-37 pubmed publisher
chromatin immunoprecipitation; brewer's yeast	Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T. Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3. Sci Rep. 2013;3:2186 pubmed publisher
immunocytochemistry; human western blot; human	Cheng J, Torkamani A, Peng Y, Jones T, Lerner R. Plasma membrane associated transcription of cytoplasmic DNA. Proc Natl Acad Sci U S A. 2012;109:10827-31 pubmed publisher
chromatin immunoprecipitation; human	Walsh H, Shupnik M. Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoter. Mol Endocrinol. 2009;23:237-50 pubmed publisher
immunoprecipitation; brewer's yeast western blot; brewer's yeast	Reid J, Svejstrup J. DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro. J Biol Chem. 2004;279:29875-8 pubmed
	Kim Y, Qi M, Dong X, Seok S, Sun H, Kemper B, et al. Transgenic mice lacking FGF15/19-SHP phosphorylation display altered bile acids and gut bacteria, promoting nonalcoholic fatty liver disease. J Biol Chem. 2023;299:104946 pubmed publisher
	Li F, Negi V, Yang P, Lee J, Ma K, Moulik M, et al. TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanism. Nucleic Acids Res. 2022;50:12723-12738 pubmed publisher
	Hensel A, Stahl P, Moews L, K xf6 nig L, Patwardhan R, H xf6 ing A, et al. The Taspase1/Myosin1f-axis regulates filopodia dynamics. iScience. 2022;25:104355 pubmed publisher
	Zhang L, Qu J, Qi Y, Duan Y, Huang Y, Zhou Z, et al. EZH2 engages TGFβ signaling to promote breast cancer bone metastasis via integrin β1-FAK activation. Nat Commun. 2022;13:2543 pubmed publisher
	Appanah R, Lones E, Aiello U, Libri D, De Piccoli G. Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability. Cell Rep. 2020;30:2094-2105.e9 pubmed publisher
	Taïb B, Aboussalah A, Moniruzzaman M, Chen S, Haughey N, Kim S, et al. Lipid accumulation and oxidation in glioblastoma multiforme. Sci Rep. 2019;9:19593 pubmed publisher
	Helsley R, Varadharajan V, Brown A, Gromovsky A, Schugar R, Ramachandiran I, et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease. elife. 2019;8: pubmed publisher
	Eng J, Thibault G, Luoh S, Gray J, Chang Y, Chin K. Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis. Methods Mol Biol. 2020;2055:521-562 pubmed publisher
	Asimakopoulou A, Vucur M, Luedde T, Schneiders S, Kalampoka S, Weiss T, et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma. Cancers (Basel). 2019;11: pubmed publisher
	Farmer B, Kluemper J, Johnson L. Apolipoprotein E4 Alters Astrocyte Fatty Acid Metabolism and Lipid Droplet Formation. Cells. 2019;8: pubmed publisher
	Chali F, Milior G, Marty S, Morin Brureau M, Le Duigou C, Savary E, et al. Lipid markers and related transcripts during excitotoxic neurodegeneration in kainate-treated mice. Eur J Neurosci. 2019;50:1759-1778 pubmed publisher
	Zhang P, Csaki L, Ronquillo E, Baufeld L, Lin J, Gutiérrez A, et al. Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid homeostasis to regulate chylomicron synthesis. J Clin Invest. 2019;129:281-295 pubmed publisher
	Larsen S, Vigelsø A, Dandanell S, Prats C, Dela F, Helge J. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. J Diabetes Res. 2018;2018:9257874 pubmed publisher
	Li Z, Rasmussen M, Li J, Henríquez Olguín C, Knudsen J, Madsen A, et al. Periodized low protein-high carbohydrate diet confers potent, but transient, metabolic improvements. Mol Metab. 2018;17:112-121 pubmed publisher
	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
	Madushani Herath K, Cho J, Kim A, Eom T, Kim J, Kim J, et al. Phenolic acid and flavonoid-rich fraction of Sasa quelpaertensis Nakai leaves prevent alcohol induced fatty liver through AMPK activation. J Ethnopharmacol. 2018;224:335-348 pubmed publisher
	Blossom S, Fernandes L, Bai S, Khare S, Gokulan K, Yuan Y, et al. Opposing Actions of Developmental Trichloroethylene and High-Fat Diet Coexposure on Markers of Lipogenesis and Inflammation in Autoimmune-Prone Mice. Toxicol Sci. 2018;164:313-327 pubmed publisher
	Clemente C, Rius C, Alonso Herranz L, Martín Alonso M, Pollán A, Camafeita E, et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun. 2018;9:910 pubmed publisher
	Covington J, Johannsen D, Coen P, Burk D, Obanda D, Ebenezer P, et al. Intramyocellular Lipid Droplet Size Rather Than Total Lipid Content is Related to Insulin Sensitivity After 8 Weeks of Overfeeding. Obesity (Silver Spring). 2017;: pubmed publisher
	Kim S, Lee J, Kim T, Kim Y, Chung K. Fat Graft Survival After Recipient Site Pretreatment With Fractional Carbon Dioxide Laser. Ann Plast Surg. 2017;79:552-557 pubmed publisher
	Li J, Mihalcioiu M, Li L, Zakikhani M, Camirand A, Kremer R. Vitamin D prevents lipid accumulation in murine muscle through regulation of PPARγ and perilipin-2 expression. J Steroid Biochem Mol Biol. 2018;177:116-124 pubmed publisher
	Rendina Ruedy E, Guntur A, Rosen C. Intracellular lipid droplets support osteoblast function. Adipocyte. 2017;6:250-258 pubmed publisher
	VigelsÃ¸ A, Gram M, Wiuff C, Hansen C, Prats C, Dela F, et al. Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. Eur J Appl Physiol. 2016;116:481-94 pubmed publisher
	Nishikawa K, Iwaya K, Kinoshita M, Fujiwara Y, Akao M, Sonoda M, et al. Resveratrol increases CD68âº Kupffer cells colocalized with adipose differentiation-related protein and ameliorates high-fat-diet-induced fatty liver in mice. Mol Nutr Food Res. 2015;59:1155-70 pubmed publisher

product information

master code :

NB200-598

SKU :

NB200-598

product name :

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

unit size :

0.1 ml (also 0.025 ml)

description :

The RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free from Novus is a mouse monoclonal antibody to RNA Polymerase II/POLR2A. This antibody reacts with human,mouse,primate (negative),yeast. The RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free has been validated for the following applications: Immunoprecipitation,Western Blot,Chemotaxis,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry/ Immunofluorescence,Flow Cytometry,ELISA,Flow (Intracellular),CyTOF-ready.

target :

RNA Polymerase II/POLR2A

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

4H8

concentration :

1 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

isotype :

IgG1

purity :

Protein G purified

species :

Human,Mouse,Primate (Negative),Yeast

specificity :

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein

theoretical molecular weight :

217 kDa

gene symbol :

POLR2A

accessionNumbers :

P24928

applications :

Chemotaxis,Immunoprecipitation,Western Blot,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry/ Immunofluorescence,Flow Cytometry,ELISA,Flow (Intracellular),CyTOF-ready

USD :

409 USD

alt names :

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

more info or order :

Novus Biologicals product webpage