p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free | Novus Biologicals NB200-174 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free

catalog :

NB200-174

quantity :

0.1 ml (also 0.025 ml)

price :

399 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

5-C3-1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Novus Biologicals product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:250; loading ...; fig s3	Deland K, Starr B, Mercer J, Byemerwa J, Crabtree D, Williams N, et al. Tumor genotype dictates radiosensitization after Atm deletion in primary brainstem glioma models. J Clin Invest. 2021;131: pubmed publisher
	Jonkhout M, Vanhessche T, Ferreira M, Verbinnen I, Withof F, Van der Hoeven G, et al. Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice. J Invest Dermatol. 2024;144:2162-2175.e12 pubmed publisher
	Weidenhammer L, Liu H, Luo L, Williams N, Deland K, Kirsch D, et al. Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination. STAR Protoc. 2023;4:102094 pubmed publisher
	Roby K, Lieberman A, Kim B, Rodr xed guez N, Posimo J, Tsang T, et al. Loss of p19Arf promotes fibroblast survival during leucine deprivation. Biol Open. 2022;11: pubmed publisher
	Maru Y, Tanaka N, Tatsumi Y, Nakamura Y, Itami M, Hippo Y. Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition. Oncogenesis. 2021;10:46 pubmed publisher
	Suzuki T, Katada E, Mizuoka Y, Takagi S, Kazuki Y, Oshimura M, et al. A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing. Nucleic Acids Res. 2021;: pubmed publisher
	Santelli A, Sun I, Eirin A, Abumoawad A, Woollard J, Lerman A, et al. Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles. J Am Heart Assoc. 2019;8:e012584 pubmed publisher
	Li F, Zeng Z, Xing S, Gullicksrud J, Shan Q, Choi J, et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf. Nat Commun. 2018;9:5452 pubmed publisher
	Baek K, Ryeom S. Detection of Oncogene-Induced Senescence In Vivo. Methods Mol Biol. 2017;1534:185-198 pubmed
	Moding E, Min H, Castle K, Ali M, Woodlief L, Williams N, et al. An extra copy of p53 suppresses development of spontaneous Kras-driven but not radiation-induced cancer. JCI Insight. 2016;1: pubmed
	Li D, Fu J, Du M, Zhang H, Li L, Cen J, et al. Hepatocellular carcinoma repression by TNF?-mediated synergistic lethal effect of mitosis defect-induced senescence and cell death sensitization. Hepatology. 2016;64:1105-20 pubmed publisher
	Lee C, Castle K, Moding E, Blum J, Williams N, Luo L, et al. Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma. Nat Commun. 2015;6:8477 pubmed publisher
	Hamilton G, Abraham A, Morton J, Sampson O, Pefani D, Khoronenkova S, et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget. 2014;5:6142-67 pubmed
	Olive V, SABIO E, Bennett M, De Jong C, Biton A, McGann J, et al. A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis. elife. 2013;2:e00822 pubmed publisher
	Shchors K, Persson A, Rostker F, Tihan T, Lyubynska N, Li N, et al. Using a preclinical mouse model of high-grade astrocytoma to optimize p53 restoration therapy. Proc Natl Acad Sci U S A. 2013;110:E1480-9 pubmed publisher
	Kim H, Kim D, Li S, Lee K, Li X, Bode A, et al. Polycomb (PcG) proteins, BMI1 and SUZ12, regulate arsenic-induced cell transformation. J Biol Chem. 2012;287:31920-8 pubmed publisher
	Hakem A, Bohgaki M, Lemmers B, Tai E, Salmena L, Matysiak Zablocki E, et al. Role of Pirh2 in mediating the regulation of p53 and c-Myc. PLoS Genet. 2011;7:e1002360 pubmed publisher
	Garcia D, Warr M, Martins C, Brown Swigart L, Passegue E, Evan G. Validation of MdmX as a therapeutic target for reactivating p53 in tumors. Genes Dev. 2011;25:1746-57 pubmed publisher
	Murphy D, Junttila M, Pouyet L, Karnezis A, Shchors K, Bui D, et al. Distinct thresholds govern Myc's biological output in vivo. Cancer Cell. 2008;14:447-57 pubmed publisher
	Bertwistle D, Sherr C. Regulation of the Arf tumor suppressor in Emicro-Myc transgenic mice: longitudinal study of Myc-induced lymphomagenesis. Blood. 2007;109:792-4 pubmed
	Williams R, Roussel M, Sherr C. Arf gene loss enhances oncogenicity and limits imatinib response in mouse models of Bcr-Abl-induced acute lymphoblastic leukemia. Proc Natl Acad Sci U S A. 2006;103:6688-93 pubmed
	Klochendler Yeivin A, Picarsky E, Yaniv M. Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex. Mol Cell Biol. 2006;26:2661-74 pubmed
	Korgaonkar C, Hagen J, Tompkins V, Frazier A, Allamargot C, Quelle F, et al. Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function. Mol Cell Biol. 2005;25:1258-71 pubmed
	Bertwistle D, Zindy F, Sherr C, Roussel M. Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein. Hybrid Hybridomics. 2004;23:293-300 pubmed

image

image 1 :

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - Whole cell protein from mouse Neuro2A and MEF cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-p19ARF in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

product information

brand :

Novus Biologicals, a Bio-Techne brand

catalog number base :

NB200-174

SKU :

NB200-174

product name :

p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free

description :

The p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free from Novus Biologicals is a rat monoclonal antibody to p19ARF/CDKN2A. This antibody reacts with golden syrian hamster (negative),human,mouse. The p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free has been validated for the following applications: Immunohistochemistry,Immunoprecipitation,Flow (Intracellular),CyTOF-ready,Knockout Validated,IF/IHC,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence.

target :

p19ARF/CDKN2A

unit size :

0.1 ml (also 0.025 ml)

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

5-C3-1

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Rat

immunogen :

A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]

isotype :

IgG2b

purity :

Protein A purified

species :

Golden Syrian Hamster (Negative),Human,Mouse

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

theoretical molecular weight :

19 kDa

gene symbol :

Cdkn2a

applications :

Immunohistochemistry,Immunoprecipitation,Flow (Intracellular),CyTOF-ready,Knockout Validated,IF/IHC,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence

USD :

399

USD 2025 :

399 USD

alt names :

alternative reading frame, ARF, ARF-INK4a, CDK4I, CDKN2A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoform 3, cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, INK4, INK4a, Ink4a/Arf, INK4a-ARF, mitochondrial smARF', MTS1, p14, p14ARF, P16, p16(INK4a), p16-INK4, p16INK4a, p16-INK4a, P19, p19 ARF , p19ARF, Pctr1

more info or order :

Novus Biologicals product webpage