p53 Antibody (PAb 240) - BSA Free | Novus Biologicals NB200-103 product information

product summary

company name :

Novus Biologicals

other brands :

IMGENEX

product type :

antibody

product name :

p53 Antibody (PAb 240) - BSA Free

catalog :

NB200-103

quantity :

0.1 ml (also 0.025 ml)

price :

409 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAb 240

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Novus Biologicals product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; loading ...; fig s3a immunohistochemistry - paraffin section; human; 1:250; loading ...; fig 1c	Li W, Gu X, Liu C, Shi Y, Wang P, Zhang N, et al. A synergetic effect of BARD1 mutations on tumorigenesis. Nat Commun. 2021;12:1243 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 4a	Khayati K, Bhatt V, Hu Z, Fahumy S, Luo X, Guo J. Autophagy compensates for Lkb1 loss to maintain adult mice homeostasis and survival. elife. 2020;9: pubmed publisher
western blot; mouse; loading ...; fig af6e	Graham N, Minasyan A, Lomova A, Cass A, Balanis N, Friedman M, et al. Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. Mol Syst Biol. 2017;13:914 pubmed publisher
immunohistochemistry - paraffin section; mouse	Biswas A, Mitchell D, Johnson D. E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis. Cancer Res. 2014;74:3369-77 pubmed publisher
immunohistochemistry - paraffin section; mouse	Lange S, Bedford E, Reh S, Wittschieben J, Carbajal S, Kusewitt D, et al. Dual role for mammalian DNA polymerase ? in maintaining genome stability and proliferative responses. Proc Natl Acad Sci U S A. 2013;110:E687-96 pubmed publisher
western blot; mouse	Jensen M, Factor V, Fantozzi A, Helin K, Huh C, Thorgeirsson S. Reduced hepatic tumor incidence in cyclin G1-deficient mice. Hepatology. 2003;37:862-70 pubmed
western blot; mouse	Asher G, Lotem J, Cohen B, Sachs L, Shaul Y. Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1. Proc Natl Acad Sci U S A. 2001;98:1188-93 pubmed
immunoprecipitation; human	Jones N, Turner J, McIlwrath A, Brown R, Dive C. Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53. Mol Pharmacol. 1998;53:819-26 pubmed
immunoprecipitation; human western blot; human	Halazonetis T, Davis L, Kandil A. Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA. EMBO J. 1993;12:1021-8 pubmed
immunohistochemistry - frozen section; human	Rubio M, von Deimling A, Yandell D, Wiestler O, Gusella J, Louis D. Accumulation of wild type p53 protein in human astrocytomas. Cancer Res. 1993;53:3465-7 pubmed
western blot; human	Bargonetti J, Manfredi J, Chen X, Marshak D, Prives C. A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein. Genes Dev. 1993;7:2565-74 pubmed
immunohistochemistry; human	Davidoff A, Humphrey P, Iglehart J, Marks J. Genetic basis for p53 overexpression in human breast cancer. Proc Natl Acad Sci U S A. 1991;88:5006-10 pubmed
immunocytochemistry; rat	Georgoff I, Martinez J, Levine A. Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein. Genes Dev. 1991;5:151-9 pubmed
immunoprecipitation; rat immunohistochemistry; rat ELISA; rat western blot; rat immunoprecipitation; mouse immunohistochemistry; mouse ELISA; mouse western blot; mouse immunoprecipitation; human immunohistochemistry; human ELISA; human western blot; human	Gannon J, Greaves R, Iggo R, Lane D. Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. EMBO J. 1990;9:1595-602 pubmed
immunohistochemistry; human	Cunningham J, Lust J, Schaid D, Bren G, Carpenter H, Rizza E, et al. Expression of p53 and 17p allelic loss in colorectal carcinoma. Cancer Res. 1992;52:1974-80 pubmed
flow cytometry; human ELISA; human	Remvikos Y, Tominaga O, Hammel P, Laurent Puig P, Salmon R, Dutrillaux B, et al. Increased p53 protein content of colorectal tumours correlates with poor survival. Br J Cancer. 1992;66:758-64 pubmed
immunocytochemistry; human	Somers K, Merrick M, Lopez M, Incognito L, Schechter G, Casey G. Frequent p53 mutations in head and neck cancer. Cancer Res. 1992;52:5997-6000 pubmed
immunohistochemistry - frozen section; human	Eccles D, Brett L, Lessells A, Gruber L, Lane D, Steel C, et al. Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. Br J Cancer. 1992;65:40-4 pubmed
	Awaji A, Rizk M, Alsaiari R, Alqahtani N, Al Qadri F, Alkorbi A, et al. Chemotherapeutic Activity of Imidazolium-Supported Pd(II) o-Vanillylidene Diaminocyclohexane Complexes Immobilized in Nanolipid as Inhibitors for HER2/neu and FGFR2/FGF2 Axis Overexpression in Breast Cancer Cells. Pharmaceuticals (Basel). 2023;16: pubmed publisher
	Mansky R, Greguske E, Yu D, Zarate N, Intihar T, Tsai W, et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease. Cell Rep. 2023;42:112198 pubmed publisher
	Caspa Gokulan R, Paulrasu K, Azfar J, El Rifai W, Que J, Boutaud O, et al. Protein adduction causes non-mutational inhibition of p53 tumor suppressor. Cell Rep. 2023;42:112024 pubmed publisher
	Anton P, Rutt L, Capper C, Orlicky D, McCullough R. Profiling the oxylipidome in aged mice after chronic ethanol feeding: Identifying lipid metabolites as drivers of hepatocyte stress. Alcohol. 2023;107:119-135 pubmed publisher
	Reis Barbosa P, Marinho T, Matsuura C, Aguila M, de Carvalho J, Mandarim de Lacerda C. The obesity and nonalcoholic fatty liver disease mouse model revisited: Liver oxidative stress, hepatocyte apoptosis, and proliferation. Acta Histochem. 2022;124:151937 pubmed publisher
	Yue W, Ma J, Xiao Y, Wang P, Gu X, Xie B, et al. The Apoptotic Resistance of BRCA1-Deficient Ovarian Cancer Cells is Mediated by cAMP. Front Cell Dev Biol. 2022;10:889656 pubmed publisher
	Radtke A, Chu C, Yaniv Z, Yao L, MARR J, Beuschel R, et al. IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues. Nat Protoc. 2022;17:378-401 pubmed publisher
	Kong N, Chen X, Feng J, Duan T, Liu S, Sun X, et al. Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1. Acta Pharm Sin B. 2021;11:4045-4054 pubmed publisher
	Fontana M, Nanni J, Ghelli Luserna Di Rorà A, Petracci E, Padella A, Ghetti M, et al. Pharmacological Inhibition of WIP1 Sensitizes Acute Myeloid Leukemia Cells to the MDM2 Inhibitor Nutlin-3a. Biomedicines. 2021;9: pubmed publisher
	Marks D, Heinen N, Bachmann L, Meermeyer S, Werner M, Gallego L, et al. Amyloid precursor protein elevates fusion of promyelocytic leukemia nuclear bodies in human hippocampal areas with high plaque load. Acta Neuropathol Commun. 2021;9:66 pubmed publisher
	Li L, Chen B, An T, Zhang H, Xia B, Li R, et al. BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-κB pathways in aging mice induced by D-galactose and NaNO2. J Ethnopharmacol. 2021;271:113810 pubmed publisher
	Wang C, Yuan W, Hu A, Lin J, Xia Z, Yang C, et al. Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury. Mol Med Rep. 2020;22:175-184 pubmed publisher
	Yang Y, Karsli Uzunbas G, Poillet Perez L, Sawant A, Hu Z, Zhao Y, et al. Autophagy promotes mammalian survival by suppressing oxidative stress and p53. Genes Dev. 2020;34:688-700 pubmed publisher
	Marinho T, Borges C, Aguila M, Mandarim de Lacerda C. Intermittent fasting benefits on alpha- and beta-cell arrangement in diet-induced obese mice pancreatic islet. J Diabetes Complications. 2020;34:107497 pubmed publisher
	Tran T, Guha R, Portugal S, Skinner J, Ongoiba A, Bhardwaj J, et al. A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation. Immunity. 2019;51:750-765.e10 pubmed publisher
	Yang X, Zhao T, Feng L, Shi Y, Jiang J, Liang S, et al. PM2.5-induced ADRB2 hypermethylation contributed to cardiac dysfunction through cardiomyocytes apoptosis via PI3K/Akt pathway. Environ Int. 2019;127:601-614 pubmed publisher
	Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018;15:909-912 pubmed publisher
	Tian H, Sun Y, He L, Yi Y, Gao X, Xu D. Single-Stranded DNA-Binding Protein 1 Abrogates Cardiac Fibroblast Proliferation and Collagen Expression Induced by Angiotensin II. Int Heart J. 2018;59:1398-1408 pubmed publisher
	Kutwin M, Sawosz E, Jaworski S, Hinzmann M, Wierzbicki M, Hotowy A, et al. Investigation of platinum nanoparticle properties against U87 glioblastoma multiforme. Arch Med Sci. 2017;13:1322-1334 pubmed publisher
	Shi M, Ma L, Zhou L, Fu P. Renal Protective Effects of 17β-Estradiol on Mice with Acute Aristolochic Acid Nephropathy. Molecules. 2016;21: pubmed
	Ge X, Pettan Brewer C, Morton J, Carter K, Fatemi S, Rabinovitch P, et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis. 2015;5:28776 pubmed publisher
	JÃ¸rgensen M, BÃ¦k R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed publisher
	Blagosklonny M, Toretsky J, Bohen S, Neckers L. Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90. Proc Natl Acad Sci U S A. 1996;93:8379-83 pubmed
	Farrell P, Allan G, Shanahan F, Vousden K, Crook T. p53 is frequently mutated in Burkitt's lymphoma cell lines. EMBO J. 1991;10:2879-87 pubmed

product information

brand :

Novus Biologicals, a Bio-Techne brand

catalog number base :

NB200-103

SKU :

NB200-103

product name :

p53 Antibody (PAb 240) - BSA Free

description :

The p53 Antibody (PAb 240) - BSA Free from Novus Biologicals is a mouse monoclonal antibody to p53. This antibody reacts with human,mouse,rat,xenopus (negative),yeast. The p53 Antibody (PAb 240) - BSA Free has been validated for the following applications: Flow (Intracellular),CyTOF-ready,Hydrolysis Assay,IF/IHC,Flow Cytometry,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence.

target :

p53

unit size :

0.1 ml (also 0.025 ml)

category :

Primary Antibodies

buffer :

PBS

clonality :

Monoclonal

clone :

PAb 240

concentration :

1.0 mg/ml

conjugate :

Unconjugated

host :

Mouse

immunogen :

Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).

isotype :

IgG1 Kappa

purity :

Protein G purified

species :

Human,Mouse,Rat,Xenopus (Negative),Yeast

specificity :

This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions

storage :

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

gene symbol :

TP53

applications :

Flow (Intracellular),CyTOF-ready,Hydrolysis Assay,IF/IHC,Flow Cytometry,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence

USD :

399

USD 2025 :

409 USD

alt names :

Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53

more info or order :

Novus Biologicals product webpage