product summary
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company name :
Novus Biologicals
other brands :
IMGENEX
product type :
antibody
product name :
HSPA8/HSC71/Hsc70 Antibody (13D3)
catalog :
NB120-2788
quantity :
100 ul (also 25 ul)
price :
459 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13D3
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 5a
Caballero B, Bourdenx M, Luengo E, Díaz A, Sohn P, Chen X, et al. Acetylated tau inhibits chaperone-mediated autophagy and promotes tau pathology propagation in mice. Nat Commun. 2021;12:2238 pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig 6b
Pajares M, Rojo A, Arias E, Díaz Carretero A, Cuervo A, Cuadrado A. Transcription factor NFE2L2/NRF2 modulates chaperone-mediated autophagy through the regulation of LAMP2A. Autophagy. 2018;14:1310-1322 pubmed publisher
  • western blot; mouse; 1:5000; fig 2
Kaushik S, Cuervo A. Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis. Nat Cell Biol. 2015;17:759-70 pubmed publisher
Fern xe1 ndez Comaduran M, Minotti S, Jacob Tomas S, Rizwan J, Larochelle N, Robitaille R, et al. Impact of histone deacetylase inhibition and arimoclomol on heat shock protein expression and disease biomarkers in primary culture models of familial ALS. Cell Stress Chaperones. 2024;29:359-380 pubmed publisher
Li J, Yao H, Zhao F, An J, Wang Q, Mu J, et al. Pycard deficiency inhibits microRNA maturation and prevents neointima formation by promoting chaperone-mediated autophagic degradation of AGO2/argonaute 2 in adipose tissue. Autophagy. 2024;20:629-644 pubmed publisher
Choi Y, Yun S, Yu J, Mun Y, Lee W, Park C, et al. Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1. Mol Metab. 2023;76:101784 pubmed publisher
Idera A, Sharkey L, Kurauchi Y, Kadoyama K, Paulson H, Katsuki H, et al. Wild-type and pathogenic forms of ubiquilin 2 differentially modulate components of the autophagy-lysosome pathways. J Pharmacol Sci. 2023;152:182-192 pubmed publisher
Rovira M, Sereda R, Pladevall Morera D, Ramponi V, Marin I, Maus M, et al. The lysosomal proteome of senescent cells contributes to the senescence secretome. Aging Cell. 2022;21:e13707 pubmed publisher
Ueda E, Ohta T, Konno A, Hirai H, Kurauchi Y, Katsuki H, et al. D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation. Cells. 2022;11: pubmed publisher
Wang D, Tan K, Zeng W, Li S, Wang Y, Xu F, et al. Hepatocellular BChE as a therapeutic target to ameliorate hypercholesterolemia through PRMT5 selective degradation to restore LDL receptor transcription. Life Sci. 2022;293:120336 pubmed publisher
Bourdenx M, Martín Segura A, Scrivo A, Rodriguez Navarro J, Kaushik S, Tasset I, et al. Chaperone-mediated autophagy prevents collapse of the neuronal metastable proteome. Cell. 2021;184:2696-2714.e25 pubmed publisher
Juste Y, Cuervo A. Analysis of Chaperone-Mediated Autophagy. Methods Mol Biol. 2019;1880:703-727 pubmed publisher
Rodriguez Rodriguez J, Lewis C, McKinley K, Sikirzhytski V, Corona J, Maciejowski J, et al. Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion. Curr Biol. 2018;28:3422-3429.e5 pubmed publisher
Seki T, Sato M, Kibe Y, Ohta T, Oshima M, Konno A, et al. Lysosomal dysfunction and early glial activation are involved in the pathogenesis of spinocerebellar ataxia type 21 caused by mutant transmembrane protein 240. Neurobiol Dis. 2018;120:34-50 pubmed publisher
Sikirzhytski V, Renda F, Tikhonenko I, Magidson V, McEwen B, Khodjakov A. Microtubules assemble near most kinetochores during early prometaphase in human cells. J Cell Biol. 2018;217:2647-2659 pubmed publisher
Ciossani G, Overlack K, Petrovic A, Huis In t Veld P, Koerner C, Wohlgemuth S, et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem. 2018;293:10084-10101 pubmed publisher
Chong T, Sarac A, Yao C, Liao L, Lyttle N, Boutros P, et al. Deregulation of the spindle assembly checkpoint is associated with paclitaxel resistance in ovarian cancer. J Ovarian Res. 2018;11:27 pubmed publisher
Gong Z, Tasset I, Díaz A, Anguiano J, Tas E, Cui L, et al. Humanin is an endogenous activator of chaperone-mediated autophagy. J Cell Biol. 2018;217:635-647 pubmed publisher
Ueno M, Suzuki J, Hirose M, Sato S, Imagawa M, Zenimaru Y, et al. Cardiac overexpression of perilipin 2 induces dynamic steatosis: prevention by hormone-sensitive lipase. Am J Physiol Endocrinol Metab. 2017;313:E699-E709 pubmed publisher
Yamauchi M, Shibata A, Suzuki K, Suzuki M, Niimi A, Kondo H, et al. Regulation of pairing between broken DNA-containing chromatin regions by Ku80, DNA-PKcs, ATM, and 53BP1. Sci Rep. 2017;7:41812 pubmed publisher
Zhang S, Eitan E, Mattson M. Early involvement of lysosome dysfunction in the degeneration of cerebral cortical neurons caused by the lipid peroxidation product 4-hydroxynonenal. J Neurochem. 2017;140:941-954 pubmed publisher
Ganesan S, Reynolds C, Hollinger K, Pearce S, Gabler N, Baumgard L, et al. Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2016;310:R1288-96 pubmed publisher
Magidson V, He J, Ault J, O Connell C, Yang N, Tikhonenko I, et al. Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells. J Cell Biol. 2016;212:307-19 pubmed publisher
Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. 2015;290:27053-66 pubmed publisher
Kanfer G, Courtheoux T, Peterka M, Meier S, Soste M, Melnik A, et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. 2015;6:8015 pubmed publisher
Magidson V, Paul R, Yang N, Ault J, O Connell C, Tikhonenko I, et al. Adaptive changes in the kinetochore architecture facilitate proper spindle assembly. Nat Cell Biol. 2015;17:1134-44 pubmed publisher
Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. 2015;26:3379-89 pubmed publisher
Kaida A, Miura M. Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells. Cancer Sci. 2015;106:1370-6 pubmed publisher
Nishikawa R, Goto Y, Kurozumi A, Matsushita R, Enokida H, Kojima S, et al. MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer. Int J Urol. 2015;22:867-77 pubmed publisher
Park C, Suh Y, Cuervo A. Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage. Nat Commun. 2015;6:6823 pubmed publisher
Bee L, Nasca A, Zanolini A, Cendron F, d Adamo P, Costa R, et al. A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy. EMBO Mol Med. 2015;7:918-29 pubmed publisher
Kett L, Stiller B, Bernath M, Tasset I, Blesa J, Jackson Lewis V, et al. α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2. J Neurosci. 2015;35:5724-42 pubmed publisher
Nakajima N, Hagiwara Y, Oike T, Okayasu R, Murakami T, Nakano T, et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS ONE. 2015;10:e0122582 pubmed publisher
Lee I, Lee C, Lee J. CaMKKβ-AMPKα2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells. Cell Cycle. 2015;14:598-611 pubmed publisher
Ferreira J, Fôfo H, Bejarano E, Bento C, Ramalho J, Girao H, et al. STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy. Autophagy. 2013;9:1349-66 pubmed publisher
Hubbi M, Hu H, Kshitiz -, Ahmed I, Levchenko A, Semenza G. Chaperone-mediated autophagy targets hypoxia-inducible factor-1? (HIF-1?) for lysosomal degradation. J Biol Chem. 2013;288:10703-14 pubmed publisher
Orenstein S, Kuo S, Tasset I, Arias E, Koga H, Fernandez Carasa I, et al. Interplay of LRRK2 with chaperone-mediated autophagy. Nat Neurosci. 2013;16:394-406 pubmed publisher
Chang A, Jeong J, Levine R. Iron regulatory protein 2 turnover through a nonproteasomal pathway. J Biol Chem. 2011;286:23698-707 pubmed publisher
Koga H, Kaushik S, Cuervo A. Altered lipid content inhibits autophagic vesicular fusion. FASEB J. 2010;24:3052-65 pubmed publisher
Welsch T, Younsi A, Disanza A, Rodriguez J, Cuervo A, Scita G, et al. Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells. Exp Cell Res. 2010;316:1914-24 pubmed publisher
Guerrero C, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, et al. Heat shock cognate protein 70 is involved in rotavirus cell entry. J Virol. 2002;76:4096-102 pubmed
product information
brand :
Novus Biologicals, a Bio-Techne brand
catalog number base :
NB120-2788
SKU :
NB120-2788
product name :
HSPA8/HSC71/Hsc70 Antibody (13D3)
description :
The HSPA8/HSC71/Hsc70 Antibody (13D3) from Novus Biologicals is a mouse monoclonal antibody to HSPA8/HSC71/Hsc70. This antibody reacts with bovine,feline,fish,hamster,human,mouse,primate,rat. The HSPA8/HSC71/Hsc70 Antibody (13D3) has been validated for the following applications: Immunohistochemistry,Immunohistochemistry-Frozen,Simple Western,Block/Neutralize,IF/IHC,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunoblotting,Immunocytochemistry/ Immunofluorescence.
target :
HSPA8/HSC71/Hsc70
unit size :
100 ul (also 25 ul)
category :
Primary Antibodies
buffer :
Ascites diluted with PBS
clonality :
Monoclonal
clone :
13D3
conjugate :
Unconjugated
host :
Mouse
immunogen :
Mouse spermatogenic cell protein.
isotype :
IgM
purity :
Unpurified
species :
Bovine,Feline,Fish,Hamster,Human,Mouse,Primate,Rat
specificity :
This displays slight cross-reactivity to HSP 70
storage :
Store at -20C. Avoid freeze-thaw cycles.
theoretical molecular weight :
70 kDa
gene symbol :
HSPA8
accessionNumbers :
P08107
applications :
Immunohistochemistry,Immunohistochemistry-Frozen,Simple Western,Block/Neutralize,IF/IHC,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunoblotting,Immunocytochemistry/ Immunofluorescence
USD :
449
USD 2025 :
459 USD
alt names :
Heat shock 70 kDa protein 8, heat shock 70kD protein 8, heat shock 70kDa protein 8, heat shock cognate 71 kDa protein, heat shock cognate protein 54, heat shock cognate protein, 71-kDa, HSC54, HSC70heat shock 70kd protein 10, HSC71, HSP71, HSP73constitutive heat shock protein 70, HSPA10, LAP1, lipopolysaccharide-associated protein 1, LPS-associated protein 1, MGC131511, MGC29929, NIP71, N-myristoyltransferase inhibitor protein 71
more info or order :
company information
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
novus@novusbio.com
https://www.novusbio.com
3037301950
headquarters: USA
Novus Biologicals licenses, manufactures, and markets antibodies to over 20,000 unique targets to support a wide array of research areas. Novus is built on honesty, collaboration and strong relationships and continues to provide quality tools that accelerate research. Every product is backed by our 100% guarantee.